组分: 22 mM Tris-HCl (pH 8.4),55 mM KCl,1.65 mM 氯化镁,220 μM dGTP,220 μM dATP,220 μM dTTP,220 μM dCTP,22U/ml 重组 Taq DNA 聚合酶和稳定剂。
引用和文献 (2)
引用和文献
Abstract
Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358.
Authors: Saitoh Hisato; Pizzi Maryann Delli; Wang Jian;
Journal:J Biol Chem
PubMed ID:11709548
Ubc9, a conjugation enzyme for the ubiquitin-related modifier SUMO, is present predominantly in the nucleus and at the nuclear pore complex. The functional significance of its subcellular compartmentalization, however, remains to be elucidated. Here, we define a Pro-Glu-Asp-Ser-Thr-rich element containing 129 amino acid residues, designated IR1+2, on the human nucleoporin ... More
Lithocholic acid decreases expression of bile salt export pump through farnesoid X receptor antagonist activity.
Authors: Yu Jinghua; Lo Jane-L; Huang Li; Zhao Annie; Metzger Edward; Adams Alan; Meinke Peter T; Wright Samuel D; Cui Jisong;
Journal:J Biol Chem
PubMed ID:12052824
Bile salt export pump (BSEP) is a major bile acid transporter in the liver. Mutations in BSEP result in progressive intrahepatic cholestasis, a severe liver disease that impairs bile flow and causes irreversible liver damage. BSEP is a target for inhibition and down-regulation by drugs and abnormal bile salt metabolites, ... More