What is the best way to handle the MEM Vitamin Solution (100X)? It comes frozen, so should I thaw it, then aliquot it and freeze the aliquots for later use?
If you're going to be using small batches of the vitamin solution at a time, it would be best to aliquot it out and store at -20 degrees C. The stability of the MEM Vitamin Solution is one year at -20 degrees C, while it is only 2 weeks at 4 degrees C.
The MEM Vitamin Solution (Cat. No. 11120-052 or 11120-037 depending on your country) is a growth supplement for cell culture medium. This solution is formulated to contain 100X the vitamins found in standard MEM. Find the complete formulation here (http://www.thermofisher.com/us/en/home/technical-resources/media-formulation.163.html).