两地 cGMP 生产和质量体系 Gibco™ Grace 补充昆虫培养基在位于纽约格兰德岛的符合 cGMP 要求的工厂内生产。该工厂是在FDA登记的医疗器械生产商,且通过ISO 13485标准认证。为确保供应链的稳定,我们同时提供由我们的苏格兰工厂生产的相同产品 (11605-045)。该工厂是在 FDA 注册的医疗器械生产商,且通过 ISO 13485 标准认证。
Grace 补充昆虫培养基含有乳清蛋白水解物和酵母粉,且在使用前通常补充 10% 胎牛血清 (FBS)。Grace 补充昆虫培养基需要环境 CO2 以维持生理 pH 值。
仅供科研使用。不可用于诊断程序。
规格
细胞系Sf21, Sf9
细胞类型昆虫细胞
无机盐钙、镁
产品线Gibco™
产品类型Grace 昆虫培养基
数量10 x 500 mL
运输条件室温
形式液体
加有添加剂谷氨酰胺, 酵母粉, 乳清蛋白水解物
不加添加剂无昆虫血淋巴
Unit SizeEach
内容与储存
储存条件:2°C 至 8°C。避光储存 运输条件:环境 有效期:自生产之日起 12 个月
常见问题解答 (FAQ)
Grace’s昆虫培养基中包含了哪些辅剂?为什么它们以单管形式提供?
补充培养基简称为TNM-FH。它包含以下成份(每500 mL):
TC酵母粉:1.66 g,乳清蛋白水解物1.66 g,L-谷氨酰胺:0.3 g
这些添加剂能够帮助增加培养基的稳定性和延长保质期。请注意这些添加剂未经消毒,须经过滤灭菌(通过0.2 µm滤器)后方可加入。
TNM-FH未加入热灭活的10% FBS之前,不视为完全培养基。血清会提供额外的养分,同时也能够保护悬浮培养条件(即旋转器和振荡培养瓶)下的细胞免受流体压力的影响。
For how long can I store Grace's Insect Medium, unsupplemented at room temperature?
We ship this product under ambient conditions throughout the year. We have performed shipping studies that shown components to be stable for at least 7 days at ambient temperatures. The important factor is to ensure that the media is not exposed to light at the warmer temperatures as there are vitamins and amino acids that will breakdown with light exposure.
What additional supplements are required in Grace's Insect Cell Culture Medium, Unsupplemented to make a "complete" formulation that will support insect cell growth?
Grace's Insect Cell Culture Medium, Unsupplemented requires addition of 3.33 g/L of lactalbumin hydrolysate and 3.33 g/L of yeastolate for the base growth medium. For the complete growth medium that will support insect cell growth, 10% fetal bovine serum must also be added. This complete growth medium is also referred to as TNM-FH.