RNA UltraSense™ 一步法定量 RT-PCR 系统
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Applied Biosystems™

RNA UltraSense™ 一步法定量 RT-PCR 系统

RNA UltraSense™ 一步法定量 RT-PCR 系统专为超低丰度转录物和 RNA 病毒的扩增和实时定量而设计。经过优化的超浓缩系统将 SuperScript™ III了解更多信息
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货号数量
11732927100 rxns
货号 11732927
价格(CNY)
-
数量:
100 rxns
RNA UltraSense™ 一步法定量 RT-PCR 系统专为超低丰度转录物和 RNA 病毒的扩增和实时定量而设计。经过优化的超浓缩系统将 SuperScript™ III RT 和 Platinum™ Taq DNA 聚合酶相结合,可提供更高的引物特异性、更高的产物产量以及更宽动态范围的检测。高浓度配方在使用低浓度/高容量 RNA 样品时提供了极大的灵活性,并且提高了在二级结构内富含靶点时的检测性能。可检出浓度低至 25 pg/µl 的总 RNA,PCR 效率高达 97%(图1)。RNA UltraSense™ 系统特点:

•SuperScript™ III RT 实现较高温度的 cDNA 合成(高达 60°C),从而在处理 RNA 二级结构时获得更大成功
• 采用热启动技术的 Platinum™ Taq DNA 聚合酶提高了特异性
• 使用 LUX™ 荧光引物或双标记荧光探针优化性能
• 专有的超浓缩缓冲液用于加入较大样品量(高达 70%)和成功处理 RNA 二级结构
• 性能已在多个实时仪器平台上得到验证
• 一步法形式,快速、便利且反应差异较小

应用:
RNA 的实时定量扩增,包括:
•RNA 病毒
• 低丰度 RNA 转录物
• 难以扩增的 RNA 靶标
• 高价值 RNA 检测和研究
仅供科研使用。不可用于诊断程序。
规格
反应次数100 次反应
聚合酶DNA 聚合酶
产品线Platinum™、SuperScript™、UltraSense™
产品类型一步法定量 RT-PCR 系统
数量100 rxns
样品类型RNA
运输条件干冰
足够用于100 次反应
检测方法引物-探针
高 GC PCR 扩增效果
PCR 方法一步法 RT-qPCR
反应速度标准
Unit SizeEach
内容与储存
RNA UltraSense™ 一步法定量 RT-PCR 系统随附以下组成部分:250 µL RNA UltraSense™ 酶混合物(包括 SuperScript™ III RT、Platinum™Taq DNA 聚合酶和 RNaseOUT™ 核糖核酸酶抑制剂);2 x 1 mL RNA UltraSense™ 5X 反应混合物;300 µL 20X 牛血清白蛋白 (BSA),超纯分子生物学级 (5 mg/mL);1 mL 50 mM 硫酸镁 (MgSO4);100 µL ROX 参比染料。提供足以进行 100 次反应的试剂。组成部分在 -20°C 下储存。可通过在 -80°C 下储存延长稳定性。ROX 参比染料必须于暗处储存。

常见问题解答 (FAQ)

What can I do to improve the sensitivity of my qPCR assay?

If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:

- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)

How do I set the baseline for my qPCR experiment?

Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).

How do I set the threshold for my qPCR experiment?

In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).

I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?

There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.

I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?

There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.