SuperScript™ VILO™ cDNA 合成试剂盒
SuperScript™ VILO™ cDNA 合成试剂盒
引用和文献 (5)
Invitrogen™

SuperScript™ VILO™ cDNA 合成试剂盒

SuperScript VILO cDNA 合成试剂盒用于生成两步法 RT-qPCR 应用所需的第一链 cDNA。该试剂盒以 2 管的形式提供，包括含有了解更多信息
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货号反应次数
1175405050 次反应
11754250250 次反应
货号 11754050
价格（CNY)
9,576.00
Each
添加至购物车
反应次数:
50 次反应
请求批量或定制报价
价格（CNY)
9,576.00
Each
添加至购物车
SuperScript VILO cDNA 合成试剂盒用于生成两步法 RT-qPCR 应用所需的第一链 cDNA。该试剂盒以 2 管的形式提供，包括含有 VILO 反应混合物的单管和含有酶混合物的单管。该酶混合物之中含有一种 SuperScript III 反转录酶 (RT)，这是一种经过基因工程改造的 MMLV RT，具有更低的 RNase H 活性、更高的热稳定性，可实现高效的 cDNA 合成。该试剂盒可用于从不同起始量的 RNA 合成 cDNA。

注：为在两步法 RT-qPCR 中实现更出色的第一链 cDNA 合成性能，我们推荐使用 SuperScript IV VILO 预混液或含 ezDNase 酶的 SuperScript IV VILO 预混液。该预混液为便利的单管预混液规格，其中包含了 cDNA 合成反应所需的所有组分。凭借高持续合成能力、热稳定的 SuperScript IV RT 及进一步优化的缓冲液，其将可靠的 VILO 技术提升至全新维度。该预混液支持在更短的时间内以更高的温度高效地进行 cDNA 合成，即便纯度欠佳或模板有限的情况下，也能够获得出色的 cDNA 得率和灵敏度。
仅供科研使用。不可用于诊断程序。
规格
最终产品类型第一链 cDNA
产品规格试剂盒
反应次数50 次反应
最佳反应温度42°C
数量50 次反应
反应形式预混液
试剂类型反转录
逆转录酶SuperScript III
核糖核酸酶 H 活性减少
运输条件干冰
尺寸（最终产品）长达 10 kb
原始材料RNA
技术反转录
检测方法qPCR
适用于（应用）实时 PCR (qPCR)
高 GC PCR 扩增效果高
反应速度60 min。
Unit SizeEach
内容与储存

• 5X VILO 反应混合物
• 10X SuperScript III 酶混合物

在 –30°C 至 –5°C 条件下储存。

常见问题解答 (FAQ)

SuperScript III反转录酶和SuperScript VILO试剂盒之中的反转录酶有何区别？

SuperScript VILO cDNA合成试剂盒含有SuperScript III反转录酶和辅助蛋白的混合物，其中后者可以提高反转录反应的效率，进而提高得率。由于存在辅助蛋白，SuperScript VILO中的反转录在42°C条件下活化。

PCR反应要加入多少第一链cDNA反应产物？

尽管实际体积取决于用于第一链合成反应的RNA起始量和靶标基因的丰度，但我们建议使用10%的第一链cDNA反应产物进行PCR反应。

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

What is the difference between SuperScript III RT and the RT in the SuperScript VILO kit?

The SuperScript VILO cDNA Synthesis Kit contains a mix of SuperScript III RT and helper proteins which help to increase the efficiency of the reverse transcription reaction and thus improve yield. The RT in the SuperScript

The SuperScript VILO cDNA Synthesis Kit (Cat. No. 11754050) contains a mix of SuperScript III RT and helper proteins which help to increase the efficiency of the reverse transcription reaction and thus improve yield. The RT in the SuperScript VILO kit is active at 42 degrees C due to the helper proteins.

How much of the first-strand cDNA reaction should I load for PCR?

While the volume is dependent on the starting amount of RNA used for the first-strand synthesis and the abundance of the target gene, we'd recommend starting with 10% of the first-strand reaction for your PCR reaction.

View all SuperScript™ VILO™ cDNA 合成试剂盒 FAQs

引用和文献 (5)

引用和文献
Abstract
A useful approach to total analysis of RISC-associated RNA.
Authors:Hayashida Y, Nishibu T, Inoue K, Kurokawa T,
Journal:BMC Res Notes
PubMed ID:19706194
'ABSTRACT: BACKGROUND: Identifying the endogenous RNA induced silencing complex(RISC)-associated RNAs is essential for understanding the cellular regulatory networks by miRNAs. Recently, isolation of RISC-associated mRNAs using antibody was reported, but their method needs a large amount of initial materials. We tried to improve the protocol and constructed an efficient and ... More
Transcriptome analysis of proliferating Arabidopsis endosperm reveals biological implications for the control of syncytial division, cytokinin signaling, and gene expression regulation.
Authors:Day RC, Herridge RP, Ambrose BA, Macknight RC,
Journal:Plant Physiol
PubMed ID:18923020
During the early stages of seed development, Arabidopsis (Arabidopsis thaliana) endosperm is syncytial and proliferates rapidly through repeated rounds of mitosis without cytokinesis. This stage of endosperm development is important in determining final seed size and is a model for studying aspects of cellular and molecular biology, such as the ... More
T cells modulate Epstein-Barr virus latency phenotypes during infection of humanized mice.
Authors:Heuts F, Rottenberg ME, Salamon D, Rasul E, Adori M, Klein G, Klein E, Nagy N,
Journal:
PubMed ID:24390326
Human B cells, the main target of Epstein-Barr virus (EBV), can display several types of latent viral protein expression, denoted 0, I, IIa, IIb, or III. Of these, only type III expression induces proliferation of cells in vitro. These latency types are present at specific stages of infection and are ... More
Decrease of miR-146b-5p in monocytes during obesity is associated with loss of the anti-inflammatory but not insulin signaling action of adiponectin.
Authors:Hulsmans M, Van Dooren E, Mathieu C, Holvoet P,
Journal:PLoS One
PubMed ID:22393448
Low adiponectin, a well-recognized antidiabetic adipokine, has been associated with obesity-related inflammation, oxidative stress and insulin resistance. Globular adiponectin is an important regulator of the interleukin-1 receptor-associated kinase (IRAK)/NF?B pathway in monocytes of obese subjects. It protects against inflammation and oxidative stress by inducing IRAK3. microRNA (miR)-146b-5p inhibits NF?B-mediated inflammation ... More
Cyclin D1 splice variants: polymorphism, risk, and isoform-specific regulation in prostate cancer.
Authors:Comstock CE, Augello MA, Benito RP, Karch J, Tran TH, Utama FE, Tindall EA, Wang Y, Burd CJ, Groh EM, Hoang HN, Giles GG, Severi G, Hayes VM, Henderson BE, Le Marchand L, Kolonel LN, Haiman CA, Baffa R, Gomella LG, Knudsen ES, Rui H, Henshall SM, Sutherland RL, Knudsen KE,
Journal:Clin Cancer Res
PubMed ID:19706803
PURPOSE: Alternative CCND1 splicing results in cyclin D1b, which has specialized, protumorigenic functions in prostate not shared by the cyclin D1a (full length) isoform. Here, the frequency, tumor relevance, and mechanisms controlling cyclin D1b were challenged. EXPERIMENTAL DESIGN: First, relative expression of both cyclin D1 isoforms was determined in prostate ... More