Search
引用和文献 (21)
Invitrogen™
Gateway™ pDEST™15 载体
为满足您所有的表达需求，Invitrogen 提供了先进的 Gateway™ 目的载体，用于在大肠杆菌、昆虫、酵母或哺乳动物细胞中进行表达，以及生成天然蛋白或 N 或 C 端融合蛋白了解更多信息
| 货号 | 数量 |
|---|---|
| 11802014 | 6 μg |
货号 11802014
价格（CNY)
5,715.21
Online Exclusive
Ends: 31-Dec-2025
6,824.00共减 1,108.79 (16%)
Each
数量:
6 μg
价格（CNY)
5,715.21
Online Exclusive
Ends: 31-Dec-2025
6,824.00共减 1,108.79 (16%)
Each
为满足您所有的表达需求，Invitrogen 提供了先进的 Gateway™ 目的载体，用于在大肠杆菌、昆虫、酵母或哺乳动物细胞中进行表达，以及生成天然蛋白或 N 或 C 端融合蛋白。无论是入门克隆还是 Ultimate™ RF 克隆，所有 Gateway™ 目的载体具有使用任何 attL-侧区片段进行重组的 attR 位点。下表列出了多种可用的目的载体。
如果需要额外材料，可单独提供：Gateway™ 入门克隆、Gateway™ LR Clonase™ 酶混合物和反应缓冲液。
如果需要额外材料，可单独提供：Gateway™ 入门克隆、Gateway™ LR Clonase™ 酶混合物和反应缓冲液。
仅供科研使用。不可用于诊断程序。
规格
耐抗生素细菌氨苄青霉素 (AmpR)
构成或诱导系统诱导
产品类型表达载体
数量6 μg
选择试剂（真核生物）无
载体pDEST、Gateway T7 载体
克隆方法Gateway™
产品线Gateway
促进剂T7
蛋白标记GST标签
Unit SizeEach
内容与储存
所有目的载体均以超螺旋化冻干形式提供。
常见问题解答 (FAQ)
可以使用BP Clonase酶和LR Clonase酶替代BP Clonase II 酶LR Clonase II酶进行BP/LR Clonase反应的一步法实验方案吗？
有推荐的一步式BP/LR重组实验方案吗？
如果丢失了入门克隆，如何将目的基因从一个Gateway兼容的表达克隆转移到一个新的目的载体？
我可以单独购买5X LR Clonase缓冲液或5X BP Clonase缓冲液吗？
是否提供用于在植物内表达的Gateway载体吗？
引用和文献 (21)
引用和文献
Abstract
A novel type of deubiquitinating enzyme.
Journal:J Biol Chem
PubMed ID:12682062
A previous report from this laboratory described two novel proteins that have sequence similarity to A20, a negative regulator of NF-kappaB (Evans, P. C., Taylor, E. R., Coadwell, J., Heyninck, K., Beyaert, R., and Kilshaw, P. J. (2001) Biochem. J. 357, 617-623). One of these molecules, cellular zinc finger anti-NF-kappaB
The ISG15 isopeptidase UBP43 is regulated by proteolysis via the SCFSkp2 ubiquitin ligase.
Journal:J Biol Chem
PubMed ID:15342634
'The Skp2 oncoprotein belongs to the family of F-box proteins that function as substrate recognition factors for SCF (Skp1, cullin, F-box protein) E3 ubiquitin-ligase complexes. Binding of the substrate to the SCFSkp2 complex catalyzes the conjugation of ubiquitin molecules to the bound substrate, resulting in multi-ubiquitination and rapid degradation by
The oncoprotein Tax binds the SRC-1-interacting domain of CBP/p300 to mediate transcriptional activation.
Journal:Mol Cell Biol
PubMed ID:11463834
'Oncogenesis associated with human T-cell leukemia virus (HTLV) infection is directly linked to the virally encoded transcription factor Tax. To activate HTLV-1 transcription Tax interacts with the cellular protein CREB and the pleiotropic coactivators CBP and p300. While extensively studied, the molecular mechanisms of Tax transcription function and coactivator utilization
Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehyde.
Journal:Proc Natl Acad Sci U S A
PubMed ID:16698923
'An important phenylalanine-derived volatile compound produced by plants is 2-phenylethanol. It is a major contributor to flavor in many foods, including fresh fruits, such as tomato, and an insect-attracting scent in roses and many other flowers. Despite the centrality of 2-phenylethanol to flavor and fragrance, the plant genes responsible for
The genes encoding Arabidopsis ORC subunits are E2F targets and the two ORC1 genes are differently expressed in proliferating and endoreplicating cells.
Journal:Nucleic Acids Res
PubMed ID:16179646
'Initiation of eukaryotic DNA replication depends on the function of pre-replication complexes (pre-RC), one of its key component being the six subunits origin recognition complex (ORC). In spite of a significant degree of conservation among ORC proteins from different eukaryotic sources, the regulation of their availability varies considerably in different