Gateway™ pDEST™8 载体

引用和文献 (5)

Invitrogen™

Gateway™ pDEST™8 载体

为满足您所有的表达需求，Invitrogen 提供了先进的 Gateway™ 目的载体，用于在大肠杆菌、昆虫、酵母或哺乳动物细胞中进行表达，以及生成天然蛋白或 N 或 C 端融合蛋白了解更多信息

货号	数量
11804010 又称 11804-010	6 μg

货号 11804010

又称 11804-010

价格（CNY)

8,763.00

添加至购物车

价格（CNY)

8,763.00

添加至购物车

为满足您所有的表达需求，Invitrogen 提供了先进的 Gateway™ 目的载体，用于在大肠杆菌、昆虫、酵母或哺乳动物细胞中进行表达，以及生成天然蛋白或 N 或 C 端融合蛋白。无论是入门克隆还是 Ultimate™ RF 克隆，所有 Gateway™ 目的载体具有使用任何 attL-侧区片段进行重组的 attR 位点。下表列出了多种可用的目的载体。

如果需要额外材料，可单独提供：Gateway™ 入门克隆、Gateway™ LR Clonase™ 酶混合物和反应缓冲液。

仅供科研使用。不可用于诊断程序。

产品类型表达载体

数量6 μg

载体pDEST、Gateway 杆状病毒载体

克隆方法Gateway™

产品线Gateway

促进剂多角体蛋白

蛋白标记未标记

Unit SizeEach

内容与储存

所有目的载体均以超螺旋化冻干形式提供。

常见问题解答 (FAQ)

可以使用BP Clonase酶和LR Clonase酶替代BP Clonase II 酶LR Clonase II酶进行BP/LR Clonase反应的一步法实验方案吗？

在BP/LR Clonase反应的一步法实验方案中，不建议用BP Clonase酶和LR Clonase酶替代BP Clonase II 酶/LR Clonase II酶，因为这样的重组效率非常低。

有推荐的一步式BP/LR重组实验方案吗？

有的，我们能提供针对BP/LR Clonase反应的一步式实验方案DNA可以在一步反应后被克隆到目的载体中，从而节省了您的时间和金钱。

如果丢失了入门克隆，如何将目的基因从一个Gateway兼容的表达克隆转移到一个新的目的载体？

建议使用一个供体载体进行一次BP反应以获得一个入门克隆。然后将这一入门克隆和目的载体进行一次LR反应以获得新的表达克隆。

我可以单独购买5X LR Clonase缓冲液或5X BP Clonase缓冲液吗？

5X LR Clonase缓冲液或5X BP Clonase缓冲液不作为单独产品出售。它们作为酶试剂盒的一部分进行销售。

是否提供用于在植物内表达的Gateway载体吗？

我们不提供任何用于在植物内表达的Gateway载体。

View all Gateway™ pDEST™8 载体 FAQs

引用和文献 (5)

引用和文献

Abstract

Target-induced formation of neuraminidase inhibitors from in vitro virtual combinatorial libraries.

Authors: HochgÃ¼rtel Matthias; Kroth Heiko; Piecha Dorothea; Hofmann Michael W; Nicolau Claude; Krause Sonja; Schaaf Otmar; Sonnenmoser Gabriele; Eliseev Alexey V;

Journal:Proc Natl Acad Sci U S A

PubMed ID:11891312

'Neuraminidase, a key enzyme responsible for influenza virus propagation, has been used as a template for selective synthesis of small subsets of its own inhibitors from theoretically highly diverse dynamic combinatorial libraries. We show that the library building blocks, aldehydes and amines, form significant amounts of the library components resulting ... More

Enhanced production of green fluorescent fusion proteins in a baculovirus expression system by addition of secretion signal.

Authors:Katagiri Y, Ingham KC.

Journal:Biotechniques

PubMed ID:12139250

All Six Modules of the Gelatin-binding Domain of Fibronectin Are Required for Full Affinity.

Authors:Katagiri Y, Brew SA, Ingham KC,

Journal:J Biol Chem

PubMed ID:12538576

The gelatin-binding sites of fibronectin are confined to a 42-kDa region having four type I and two type II modules in the following order: I(6)-II(1)-II(2)-I(7)-I(8)-I(9). To determine the relative importance of each module for recognition of gelatin, recombinant green fluorescent fusion proteins were prepared in which individual modules or groups ... More

Cleavage of von Willebrand Factor Requires the Spacer Domain of the Metalloprotease ADAMTS13.

Authors:Zheng X, Nishio K, Majerus EM, Sadler JE,

Journal:J Biol Chem

PubMed ID:12791682

ADAMTS13 consists of a reprolysin-type metalloprotease domain followed by a disintegrin domain, a thrombospondin type 1 motif (TSP1), Cys-rich and spacer domains, seven more TSP1 motifs, and two CUB domains. ADAMTS13 limits platelet accumulation in microvascular thrombi by cleaving the Tyr1605-Met1606 bond in von Willebrand factor, and ADAMTS13 deficiency causes ... More

DNA cloning using in vitro site-specific recombination.

Authors: Hartley J L; Temple G F; Brasch M A;

Journal:Genome Res

PubMed ID:11076863

As a result of numerous genome sequencing projects, large numbers of candidate open reading frames are being identified, many of which have no known function. Analysis of these genes typically involves the transfer of DNA segments into a variety of vector backgrounds for protein expression and functional analysis. We describe ... More