引用和文献 (6)

Invitrogen™

Gateway™ pDEST™10 载体

为满足您所有的表达需求，Invitrogen 提供了先进的 Gateway™ 目的载体，用于在大肠杆菌、昆虫、酵母或哺乳动物细胞中进行表达，以及生成天然蛋白或 N 或 C 端融合蛋白了解更多信息

货号	数量
11806015	6 μg

货号 11806015

价格（CNY)

8,630.00

价格（CNY)

8,630.00

为满足您所有的表达需求，Invitrogen 提供了先进的 Gateway™ 目的载体，用于在大肠杆菌、昆虫、酵母或哺乳动物细胞中进行表达，以及生成天然蛋白或 N 或 C 端融合蛋白。无论是入门克隆还是 Ultimate™ RF 克隆，所有 Gateway™ 目的载体具有使用任何 attL-侧区片段进行重组的 attR 位点。下表列出了多种可用的目的载体。

如果需要额外材料，可单独提供：Gateway™ 入门克隆、Gateway™ LR Clonase™ 酶混合物和反应缓冲液。

仅供科研使用。不可用于诊断程序。

切割TEV 蛋白酶识别位点

产品类型Gateway 系统目的表达载体

数量6 μg

载体pDEST、Gateway 杆状病毒载体

克隆方法Gateway™

产品线Gateway

促进剂多角体蛋白

蛋白标记His 标签 (6x)

Unit SizeEach

内容与储存

所有目的载体均以超螺旋化冻干形式提供。

常见问题解答 (FAQ)

可以使用BP Clonase酶和LR Clonase酶替代BP Clonase II 酶LR Clonase II酶进行BP/LR Clonase反应的一步法实验方案吗？

在BP/LR Clonase反应的一步法实验方案中，不建议用BP Clonase酶和LR Clonase酶替代BP Clonase II 酶/LR Clonase II酶，因为这样的重组效率非常低。

有推荐的一步式BP/LR重组实验方案吗？

有的，我们能提供针对BP/LR Clonase反应的一步式实验方案DNA可以在一步反应后被克隆到目的载体中，从而节省了您的时间和金钱。

如果丢失了入门克隆，如何将目的基因从一个Gateway兼容的表达克隆转移到一个新的目的载体？

建议使用一个供体载体进行一次BP反应以获得一个入门克隆。然后将这一入门克隆和目的载体进行一次LR反应以获得新的表达克隆。

我可以单独购买5X LR Clonase缓冲液或5X BP Clonase缓冲液吗？

5X LR Clonase缓冲液或5X BP Clonase缓冲液不作为单独产品出售。它们作为酶试剂盒的一部分进行销售。

是否提供用于在植物内表达的Gateway载体吗？

我们不提供任何用于在植物内表达的Gateway载体。

View all Gateway™ pDEST™10 载体 FAQs

引用和文献 (6)

引用和文献

Abstract

Munc13-4 is a GTP-Rab27-binding protein regulating dense core granule secretion in platelets.

Authors:Shirakawa R, Higashi T, Tabuchi A, Yoshioka A, Nishioka H, Fukuda M, Kita T, Horiuchi H,

Journal:J Biol Chem

PubMed ID:14699162

'Platelets store self-agonists such as ADP and serotonin in dense core granules. Although exocytosis of these granules is crucial for hemostasis and thrombosis, the underlying mechanism is not fully understood. Here, we show that incubation of permeabilized platelets with unprenylated active mutant Rab27A-Q78L, wild type Rab27A, and Rab27B inhibited the ... More

SCAP ligands are potent new lipid-lowering drugs.

Authors: Grand-Perret T; Bouillot A; Perrot A; Commans S; Walker M; Issandou M;

Journal:Nat Med

PubMed ID:11726962

'Upregulation of low-density lipoprotein receptor (LDLr) is a key mechanism to control elevated plasma LDL-cholesterol levels. Here we identify a new class of compounds that directly binds to the sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP). We show that a 14C-labeled, photo-activatable analog specifically labeled both SCAP and a ... More

Enhanced production of green fluorescent fusion proteins in a baculovirus expression system by addition of secretion signal.

Authors:Katagiri Y, Ingham KC.

Journal:Biotechniques

PubMed ID:12139250

DNA cloning using in vitro site-specific recombination.

Authors: Hartley J L; Temple G F; Brasch M A;

Journal:Genome Res

PubMed ID:11076863

As a result of numerous genome sequencing projects, large numbers of candidate open reading frames are being identified, many of which have no known function. Analysis of these genes typically involves the transfer of DNA segments into a variety of vector backgrounds for protein expression and functional analysis. We describe ... More

Role of the G protein gamma subunit in beta gamma complex modulation of phospholipase Cbeta function.

Authors: Akgoz Muslum; Azpiazu Inaki; Kalyanaraman Vani; Gautam N;

Journal:J Biol Chem

PubMed ID:11914377

The G protein betagamma complex regulates a wide range of effectors, including the phospholipase C isozymes (PLCbetas). Different domains on the beta subunit are known to contact phospholipase Cbeta and affect its regulation. In contrast, the role of the gamma subunit in Gbetagamma modulation of PLCbeta function is not known. ... More

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