CHO-S-SFM II（含次黄嘌呤和胸苷）
CHO-S-SFM II（含次黄嘌呤和胸苷）
引用和文献 (3)
Gibco™

CHO-S-SFM II（含次黄嘌呤和胸苷）

CHO-S-SFM II 是完整的无血清低蛋白（少于 100 μg/ml）培养基，适合用于中国仓鼠卵巢 (CHO) 细胞悬浮培养物的生长和维持以及使用 CHO了解更多信息
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货号数量
12052114500 mL
120520981 L
货号 12052114
价格（CNY)
1,215.00
Each
添加至购物车
数量:
500 mL
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价格（CNY)
1,215.00
Each
添加至购物车
CHO-S-SFM II 是完整的无血清低蛋白（少于 100 μg/ml）培养基，适合用于中国仓鼠卵巢 (CHO) 细胞悬浮培养物的生长和维持以及使用 CHO 细胞表达重组蛋白（1、2）。 这种即用型培养基适合用于在分批、连续和灌注培养系统中悬浮培养 CHO 和其它细胞。

CHO 培养基 (CHO Medium) 主文档已提交给 FDA。 联系技术服务部或者当地销售代表可获得对主文档进行交叉引用的许可。

含有 L-谷氨酰胺。
仅供科研使用。不可用于诊断程序。
规格
细胞系CHO
最大浓度1 X
内毒素水平低
产品规格Bottle(s)
所含抗生素不含抗生素
产品线Gibco
产品类型无血清培养基 (SFM)
数量500 mL
运输条件Room Temperature
分类Low Protein, 无血清
培养类型悬浮液细胞培养
形式液体
血清水平无血清
加有添加剂Glutamine, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate, Thymidine, Hypoxanthine
Unit SizeEach
内容与储存
Store in refrigerator (2–8°C) and protect from light.

常见问题解答 (FAQ)

Which serum-free medium should I select for my CHO cells?

Thermo Fisher Scientific offers multiple serum free media (SFM) and chemically defined (CD) media options for CHO cultures. For adherent cultures, CD CHO (10743-011) and CD OptiCHO medium (12681-011) are robust media; both are protein free and animal origin free (AOF). For adherent cultures, CD CHO-A (097-0182DJ) is a widely used protein free and animal origin free medium. There are other formulations available to suit other research needs; contact Technical Support for further information.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用和文献 (3)

引用和文献
Abstract
Steroid ligands bind human sex hormone-binding globulin in specific orientations and produce distinct changes in protein conformation.
Authors: Grishkovskaya Irina; Avvakumov George V; Hammond Geoffrey L; Catalano Maria G; Muller Yves A;
Journal:J Biol Chem
PubMed ID:12065592
'The amino-terminal laminin G-like domain of human sex hormone-binding globulin (SHBG) contains a single high affinity steroid-binding site. Crystal structures of this domain in complex with several different steroid ligands have revealed that estradiol occupies the SHBG steroid-binding site in an opposite orientation when compared with 5 alpha-dihydrotestosterone or C19 ... More
Stable production of a human growth hormone antagonist from CHO cells adapted to serum-free suspension culture.
Authors: Haldankar R; Kopchick J J; Ridgway D;
Journal:Biotechnol Prog
PubMed ID:10356250
Human growth hormone (hGH) is a polypeptide with 191 amino acids and a molecular mass of 22 kDa. An hGH analogue was created with a single amino acid substitution (glycine[G] 120 to arginine[R]) in the third alpha-helix of the hGH molecule. This hGH analogue, named hGHG120R, was found to be ... More
Expression of human thyrotropin in cell lines with different glycosylation patterns combined with mutagenesis of specific glycosylation sites. Characterization of a novel role for the oligosaccharides in the in vitro and in vivo bioactivity.
Authors: Grossmann M; Szkudlinski M W; Tropea J E; Bishop L A; Thotakura N R; Schofield P R; Weintraub B D;
Journal:J Biol Chem
PubMed ID:7493973
We used a novel approach to study the role of the Asn-linked oligosaccharides for human thyrotropin (hTSH) activity. Mutagenesis of Asn (N) within individual glycosylation recognition sequences to Gln (Q) was combined with expression of wild type and mutant hTSH in cell lines with different glycosylation patterns. The in vitro ... More