AIM V™ 培养基,液体(研究级)
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AIM V™ 培养基,液体(研究级)
Gibco™

AIM V™ 培养基,液体(研究级)

含 L-谷氨酰胺、50 µg/mL 硫酸链霉素和 10 µg/mL 硫酸庆大霉素了解更多信息
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货号数量
120550831000 mL
12055091500 mL
货号 12055083
价格(CNY)
2,831.00
Each
添加至购物车
数量:
1000 mL
Customize this product
价格(CNY)
2,831.00
Each
添加至购物车
含 L-谷氨酰胺、50 µg/mL 硫酸链霉素和 10 µg/mL 硫酸庆大霉素。
仅供科研使用。不可用于诊断程序。
规格
细胞类型单核细胞、树突状细胞、T 细胞、杂交瘤细胞、PBMC、成纤维细胞、巨噬细胞、骨髓瘤细胞、淋巴细胞
所含抗生素硫酸链霉素、庆大霉素
产品线AIM V
产品类型无血清培养基 (SFM)
纯度或质量等级研究级
数量1000 mL
运输条件室温
培养类型哺乳动物细胞培养
形式液体
加有添加剂谷氨酰胺, 酚红, HSA
Unit SizeEach
内容与储存
避光储存在冷藏冰箱 (2-8°C) 中。

常见问题解答 (FAQ)

What is the difference between AIM V Medium (Cat. Nos. 12055083, 12055091) and CTS AIM V SFM (Cat. Nos. 0870112DK, 0870112BK)?

The difference is in the intended usage of the two media. AIM V Medium (Cat. Nos. 12055083, 12055091) is for research use only whereas CTS AIM V SFM (Cat. Nos. 0870112DK, 0870112BK) is for research use or manufacturing of cell, gene, or tissue-based products.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free medium would you recommend for culture and expansion of mouse T cells?

You can try using AIM V Medium (Cat. No. 12055083 or 12055091) for growth of mouse T cells. For mouse cells, we recommend adding 50 µM beta-mercaptoethanol (Cat. No. 21985023).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

引用和文献 (26)

引用和文献
Abstract
Low oxygen tension and autologous plasma enhance T-cell proliferation and CD49d expression density in serum-free media.
Authors: Haddad H; Papoutsakis E T;
Journal:Cytotherapy
PubMed ID:11953028
'Background As cellular immunotherapy with ex vivo expanded cells becomes more widely used to treat a variety of illnesses, optimization of culture parameters, to maximize cell production and function, is essential for continued success. The effects of reduced oxygen tension and autologous plasma on T-cell expansion, receptor expression, apoptosis, and ... More
Effects of interleukin 4 upon human tumoricidal cells obtained from patients bearing solid tumors.
Authors: Jadus M R; Good R W; Crumpacker D B; Yannelli J R;
Journal:J Leukoc Biol
PubMed ID:1991997
'The use of human interleukin 4 (IL4) in the generation of tumoricidal effector cells derived from cancer patients undergoing either interleukin 2/lymphokine activated killer cells (IL2/LAK) therapy or tumor derived activated cell (TDAC) therapy was examined in the present study. Human IL4 alone did not generate LAK cells from patients ... More
T(84) epithelial cells respond to 5-hydroxytryptamine when grown in serum-free media.
Authors: Burleigh D; Fernandes K; Perrett D;
Journal:Eur J Pharmacol
PubMed ID:10708712
The aim of this study was to establish the cause of insensitivity of T(84) human colonic epithelial cells to 5-hydroxytryptamine (5-HT). Monolayers of T(84) cells were placed in modified Ussing chambers for measurement of short-circuit current, an index of secretion. When grown in serum-supplemented media, T(84) cells gave secretory responses ... More
Generation of human autologous melanoma-specific cytotoxic T cells from tumor-involved lymph nodes.
Authors: Maeda K; Lafreniere R; McKinnon J G; Jerry L M;
Journal:Mol Biother
PubMed ID:1910625
Cytotoxic T lymphocytes (CTL) specific for autologous human melanoma have been successfully generated in vitro from tumor bearing lymph nodes without any stimulation by the autologous tumor. Tumor-involved lymph node cells (LNC) were cultured in serum free medium (AIM-V) containing 1,000 U/ml of recombinant interleukin-2. The best expansion and specific ... More
Extracellular mRNA induces dendritic cell activation by stimulating tumor necrosis factor-alpha secretion and signaling through a nucleotide receptor.
Authors: Ni Houping; Capodici John; Cannon Georgetta; Communi Didier; Boeynaems Jean-Marie; Karikó Katalin; Weissman Drew;
Journal:J Biol Chem
PubMed ID:11821398
We previously demonstrated that dendritic cell (DC) pulsing with antigen-encoded mRNA resulted in the loading of both major histocompatibility complex class I and II antigen presentation pathways and the delivery of an activation signal. Coculture of mRNA-pulsed DC with T cells led to the induction of a potent primary immune ... More