CD 杂交瘤 AGT™ 培养基
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Gibco™

CD 杂交瘤 AGT™ 培养基

Gibco® CD Hybridoma Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth了解更多信息
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货号数量
12372003
又称 12372-003
10 kg
货号 12372003
又称 12372-003
价格(CNY)
277,457.00
Each
添加至购物车
数量:
10 kg
Customize this product
价格(CNY)
277,457.00
Each
添加至购物车
Gibco® CD Hybridoma Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® CD Hybridoma Medium features:
• Ability to support suspension and stationary hybridomas and myelomas
• Formulated without L-glutamine for stability
• Animal origin-free, protein-free and chemically defined formulation
• Convenient, easy-to-use Advanced Granulation Technology (AGT™) format

Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® CD Hybridoma Medium provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250x Cholesterol Lipid Concentrate.

Formulated without L-glutamine for stability
Gibco® CD Hybridoma Medium requires the addition of 8 mM L-glutamine or GlutaMAX™ supplement before use. GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Animal origin-free, protein-free and chemically defined formulation
Gibco® CD Hybridoma Medium is animal origin-free, protein-free and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates or components of unknown composition.

Convenient, easy-to-use Advanced Granulation Technology (AGT™) format
Our Advanced Granulation Technology™ (AGT™) format is an easy-to-use dry powder format. The granules dissolve instantly and pH-adjusts for fast media preparation. Complete reconstitution instructions are available in the product manual below.

Product Use
For research use/further cell culture manufacturing. Not for use in diagnostic procedures. Customers using Gibco® CD Hybridoma Medium in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco® CD Hybridoma Medium is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
仅供科研使用。不可用于诊断程序。
规格
添加剂丙酮酸钠
缓冲液碳酸氢钠,不含 HEPES
细胞类型骨髓瘤细胞、杂交瘤
分类非动物源性、化学成分明确、无蛋白
最大浓度1X
培养环境CO2
内毒素水平
形式粉末状 (高级颗粒技术,AGT)
葡萄糖浓度3.66 g/L
谷氨酰胺无含谷氨酰胺
无机盐钙、镁、磷酸钠
酚红指示剂无酚红
产品线AGT, Gibco
产品类型CD 杂交瘤培养基
纯度或质量等级认证级
数量10 kg
血清水平无血清
运输条件干冰
无菌未灭菌
Unit SizeEach
内容与储存
储存条件:2-8°C,避光干燥
运输条件:凝胶包装
有效期:自生产之日起 24 个月

常见问题解答 (FAQ)

使用AGT的主要优势是什么?

完整的配方

-微量成份的均匀分布
-以干燥形式提供完全而又复杂的配方,包括无血清,无蛋白和化学成分确定的营养培养基
-预先调整至合适的pH值与渗透压
-对于L-谷氨酰胺依赖系统,只需加入标准的L-谷氨酰胺添加剂即可
-可根据目录配方进行改良和按照客户的专有配方进行定制
颗粒形式

-迅速溶解以实现更短的培养基制备时间
-使用无尘颗粒能够减少杂质和环境污染
-与液体培养基相同的使用性能
-改进工作流程
-通过减少组分数量来帮助降低整个工艺周期成本,节约原料计划、采购和测试方面所需的时间
-更快更方便地制备培养基,潜在的错误发生率更低
-从2 kg到6000 kg的批量生产能力,帮助用户更方便地实现从研发向生产的拓展
-多种包装规格可选

何为AGT?

颗粒状细胞培养基是一类以颗粒形式提供,便于使用的干式培养基,我们将流化床造粒技术应用于细胞营养培养基制造领域,从而生产出此款产品。这一专利工艺能够将各类无血清、无蛋白和化学成分确定配方的培养基制成完整的干式颗粒状产品。
AGT能够为用户提供与液体培养基相同的培养效果,同时减少了成本、储存和运输方面的问题。该产品是一种完全培养基,pH与渗透压均经过预先调整。由于颗粒吸水迅速,因此制备培养基的过程比传统干粉培养基更快捷。AGT培养基能够帮助用户减少整个周期的运行成本,减少原料计划、采购、测试以及培养基制备所需的时间和成本。本品不仅保持了与液体培养基同等的细胞生长效果,还成功将以上优势变成了现实。

What are the main benefits of using AGT?

Complete formulations
- Homogeneous distribution of minute components
- Complete complex formulations in a dry format, including serum-free, protein-free, and chemically defined nutrient media
- Pre-adjusted to the appropriate pH and osmolality
- Require only standard supplementation with L-glutamine for L-glutamine dependent systems
- Can be customized for modified catalog and customers' proprietary formulations

Granular format
- Dissolves instantly for faster media preparation times
- Dust-free granules reduce mess and environmental contamination
- Performance equivalent to liquid media
- Workflow improvement
- Helps reduce total cycle costs due to lower number of components, decreasing time involved in raw material planning, procurement, and testing
- Faster and easier media preparation, with less potential for error
- Easy to scale up from research through production, in batch sizes from 2 kg to 6,000 kg
- Available in various packaging options

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is AGT?

Granulated cell culture media is an easy-to-use, granular form of dry media that is produced by applying fluid bed granulation technology to cell culture nutrient media manufacturing. The patented process allows production of complete formulations of a variety of serum-free, protein-free, and chemically defined media in a dry format.

AGT offers the benefits of liquid media without the cost, storage, and transportation issues. It is a complete medium that is pH and osmolality pre-adjusted. The granules dissolve instantly for faster media preparation time than conventional dry powder media. AGT media can therefore help reduce total cycle costs, decreasing time involved in raw material planning, procurement, and testing as well as media preparation. These benefits are all realized while maintaining comparable cell growth to liquid media.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.