Serum-free media for the growth of primary bovine myoblasts.
Authors:Kolkmann AM,Post MJ,Rutjens MAM,van Essen ALM,Moutsatsou P
Journal:Cytotechnology
PubMed ID:31884572
The demand for meat is expected to exceed production capacity by livestock in the coming decennia. Therefore, cultured beef might be a viable alternative to traditional livestock-derived beef. One of the problems however is the sustainability of cultured beef through the use of fetal bovine serum. We aimed to identify ... More
Viral complementation allows HIV-1 replication without integration.
BACKGROUND: The integration of HIV-1 DNA into cellular chromatin is required for high levels of viral gene expression and for the production of new virions. However, the majority of HIV-1 DNA remains unintegrated and is generally considered a replicative dead-end. A limited amount of early gene expression from unintegrated DNA ... More
miR-452-5p suppressed the metastasis of Non-small cell lung cancer through regulating Moesin.
Authors:Zhuang J,Fan J,Zhu L,Zhao L,Huang Y,Pan X,Guo T
Journal:Journal of Cancer
PubMed ID:37497401
Background: Non-small cell lung cancer (NSCLC) is a common malignant tumor, and it is characterized by high mortality. MicroRNA-452-5p (miR-452-5p) and Moesin (MSN) have been proved to be related with regulation of tumors. If miR-452-5p could regulate NSCLC through targeting MSN remain unclear. Methods: TargetScan data and GEPIA databases were ... More
Down-regulation of miR-424 inhibited the metastasis of endometrial carcinoma via targeting PTEN/PI3K/AKT signaling pathway.
Authors:Lu Y,Lin Q,Lin C,Chen J,Jiang X,He H
Journal:Journal of Cancer
PubMed ID:37781075
Background: The incidence of endometrial carcinoma (EC) has been increasing annually, and treatment of advanced cases remains challenging. MicroRNA-424 (miR-424) was reported to affect several types of tumors, but its role in EC has not been studied. Methods: We generated transient knockdown models of miR-424 and PTEN in EC cells. ... More
An in vivo model of human small intestine using pluripotent stem cells.
Differentiation of human pluripotent stem cells (hPSCs) into organ-specific subtypes offers an exciting avenue for the study of embryonic development and disease processes, for pharmacologic studies and as a potential resource for therapeutic transplant(1,2). To date, limited in vivo models exist for human intestine, all of which are dependent upon ... More