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引用和文献 (10)
Invitrogen™
Lumio™ 绿色细胞内检测试剂盒
Lumio™ 技术设计用于重组蛋白的简单荧光检测。Lumio™ 标签是结合荧光底物的小（六个氨基酸）序列，允许您在哺乳动物细胞中可视化检测蛋白定位。哺乳动物 Lumio™ Gateway™ 载体了解更多信息
| 货号 | 数量 |
|---|---|
| 12589057 | 1 kit |
货号 12589057
价格（CNY)
19,522.49
飞享价
Ends: 31-Dec-2025
23,313.00共减 3,790.51 (16%)
Each
数量:
1 kit
价格（CNY)
19,522.49
飞享价
Ends: 31-Dec-2025
23,313.00共减 3,790.51 (16%)
Each
Lumio™ 技术设计用于重组蛋白的简单荧光检测。Lumio™ 标签是结合荧光底物的小（六个氨基酸）序列，允许您在哺乳动物细胞中可视化检测蛋白定位。哺乳动物 Lumio™ Gateway™ 载体（图 1）具有以下特点：
•Lumio™ 标签用于准确的体外和体内蛋白检测
• CMV 启动子用于高水平组成型表达
• tR 位点用于与 Gateway™ 入门克隆高效重组
• 杀稻瘟菌素抗性基因用于快速高效选择
使用哺乳动物 Lumio™ Gateway™ 载体进行哺乳动物细胞中的可靠且一致的蛋白表达、检测和定位（图 2）。载体可用于生成带有 N 和 C 端 Lumio™ 融合标签的蛋白。此外，各哺乳动物 Lumio™ Gateway™ 试剂盒包括 Lumio™ 绿色、红色或双色细胞内检测试剂盒。双色细胞内检测试剂盒适用于进行两种颜色“脉冲追踪”实验，以检查随时间推移的蛋白定位。
•Lumio™ 标签用于准确的体外和体内蛋白检测
• CMV 启动子用于高水平组成型表达
• tR 位点用于与 Gateway™ 入门克隆高效重组
• 杀稻瘟菌素抗性基因用于快速高效选择
使用哺乳动物 Lumio™ Gateway™ 载体进行哺乳动物细胞中的可靠且一致的蛋白表达、检测和定位（图 2）。载体可用于生成带有 N 和 C 端 Lumio™ 融合标签的蛋白。此外，各哺乳动物 Lumio™ Gateway™ 试剂盒包括 Lumio™ 绿色、红色或双色细胞内检测试剂盒。双色细胞内检测试剂盒适用于进行两种颜色“脉冲追踪”实验，以检查随时间推移的蛋白定位。
仅供科研使用。不可用于诊断程序。
规格
颜色绿色
检测定位细胞内检测
检测方法荧光
适用于（设备）荧光显微镜、电子显微镜
标签或染料Lumio™ Green
产品类型细胞内检测试剂盒
数量1 kit
产品线Lumio
蛋白标记Lumio
Unit SizeEach
内容与储存
Lumio 细胞内检测试剂盒包含 40 µL Lumio 绿色或红色荧光试剂，以 DMSO 中的 2 mM 储备液形式提供。储存在 -20°C 下。妥善储存时，可保证稳定储存 6 个月。
常见问题解答 (FAQ)
Lumio试剂具有透膜能力么？
在使用Lumio标记哺乳动物细胞的过程中，BSA会有背景干扰吗？
Lumio Red（红）与Green（绿）试剂对细胞有毒性吗？
Lumio染色与GeneBLAzer检测相比效果如何？GFP作为目的基因的检测手段效果如何？
Is the Lumio reagent membrane-permeable?
引用和文献 (10)
引用和文献
Abstract
Dynamic fluorescent imaging of human immunodeficiency virus type 1 gag in live cells by biarsenical labeling.
Journal:J Virol
PubMed ID:15767407
'Human immunodeficiency virus type 1 (HIV-1) Gag is the primary structural protein of the virus and is sufficient for particle formation. We utilized the recently developed biarsenical-labeling method to dynamically observe HIV-1 Gag within live cells by adding a tetracysteine tag (C-C-P-G-C-C) to the C terminus of Gag in both
Stimulation of hepatitis C virus (HCV) nonstructural protein 3 (NS3) helicase activity by the NS3 protease domain and by HCV RNA-dependent RNA polymerase.
Journal:J Virol
PubMed ID:15994762
'Hepatitis C virus (HCV) nonstructural protein 3 (NS3) possesses multiple enzyme activities. The N-terminal one-third of NS3 primarily functions as a serine protease, while the remaining two-thirds of NS3 serve as a helicase and nucleoside triphosphatase. Whether the multiple enzyme activities of NS3 are functionally interdependent and/or modulated by other
Monitoring protein stability and aggregation in vivo by real-time fluorescent labeling.
Journal:Proc Natl Acad Sci U S A
PubMed ID:14701904
'In vivo fluorescent labeling of an expressed protein has enabled the observation of its stability and aggregation directly in bacterial cells. Mammalian cellular retinoic acid-binding protein I (CRABP I) was mutated to incorporate in a surface-exposed omega loop the sequence Cys-Cys-Gly-Pro-Cys-Cys, which binds specifically to a biarsenical fluorescein dye (FlAsH).
Multicolor and electron microscopic imaging of connexin trafficking.
Journal:Science
PubMed ID:11964472
'Recombinant proteins containing tetracysteine tags can be successively labeled in living cells with different colors of biarsenical fluorophores so that older and younger protein molecules can be sharply distinguished by both fluorescence and electron microscopy. Here we used this approach to show that newly synthesized connexin43 was transported predominantly in
Visualization of mRNA translation in living cells.
Journal:J Cell Biol
PubMed ID:17030983
The role of mRNA localization is presumably to effect cell asymmetry by synthesizing proteins in specific cellular compartments. However, protein synthesis has never been directly demonstrated at the sites of mRNA localization. To address this, we developed a live cell method for imaging translation of beta-actin mRNA. Constructs coding for