IPTG：

IPTG（异丙基硫代-β-半乳糖苷）是细菌中 β-半乳糖苷酶活性的诱导剂，适用于与 X-gal 或 bluo-gal 配合使用以检测克隆程序中的 lac 基因活性了解更多信息

货号	数量
15529019	1 g

货号 15529019

价格（CNY)

700.00

添加至购物车

价格（CNY)

700.00

添加至购物车

IPTG（异丙基硫代-β-半乳糖苷）是细菌中 β-半乳糖苷酶活性的诱导剂，适用于与 X-gal 或 bluo-gal 配合使用以检测克隆程序中的 lac 基因活性。

原理
IPTG 与 lac 抑制子结合并从 lac 操纵基因释放 lac 抑制子，从而使得 lac 操纵子中的基因得以转录，如编码 β-半乳糖苷酶的基因。

仅供科研使用。不可用于诊断程序。

化学名称或材料IPTG （异丙基 β-D-硫代吡喃半乳糖苷）

数量1 g

运输条件经批准可在室温下或置于湿冰上运输

产品规格Bottle

Unit SizeEach

内容与储存

包含 1 g IPTG 粉末。保存于 -20°C。

引用和文献 (3)

引用和文献

Abstract

An Escherichia coli mutant lacking the cold shock-induced palmitoleoyltransferase of lipid A biosynthesis: absence of unsaturated acyl chains and antibiotic hypersensitivity at 12 degrees C.

Authors: Vorachek-Warren Mara K; Carty Sherry M; Lin Shanhua; Cotter Robert J; Raetz Christian R H;

Journal:J Biol Chem

PubMed ID:11830594

'An acyltransferase induced by cold shock in Escherichia coli, designated LpxP, incorporates a palmitoleoyl moiety into nascent lipid A in place of the secondary laurate chain normally added by LpxL(HtrB) (Carty, S. M., Sreekumar, K. R., and Raetz, C. R. H. (1999) J. Biol. Chem. 274, 9677-9685). To determine whether ... More

Identification of endopeptidase genes from the genomic sequence of Lactobacillus helveticus CNRZ32 and the role of these genes in hydrolysis of model bitter peptides.

Authors:Sridhar VR, Hughes JE, Welker DL, Broadbent JR, Steele JL,

Journal:Appl Environ Microbiol

PubMed ID:15932998

Genes encoding three putative endopeptidases were identified from a draft-quality genome sequence of Lactobacillus helveticus CNRZ32 and designated pepO3, pepF, and pepE2. The ability of cell extracts from Escherichia coli DH5alpha derivatives expressing CNRZ32 endopeptidases PepE, PepE2, PepF, PepO, PepO2, and PepO3 to hydrolyze the model bitter peptides, beta-casein (beta-CN) ... More

Genes Encoding Calmodulin-binding Proteins in the Arabidopsis Genome.

Authors: Reddy Vaka S; Ali Gul S; Reddy Anireddy S N;

Journal:J Biol Chem

PubMed ID:11782485

Analysis of the recently completed Arabidopsis genome sequence indicates that approximately 31% of the predicted genes could not be assigned to functional categories, as they do not show any sequence similarity with proteins of known function from other organisms. Calmodulin (CaM), a ubiquitous and multifunctional Ca(2+) sensor, interacts with a ... More