A duplex PCR-based assay for measuring the amount of bacterial contamination in a nucleic acid extract from a culture of free-living protists.
Authors:Marron AO, Akam M, Walker G
Journal:PLoS One
PubMed ID:23593495
'Cultures of heterotrophic protists often require co-culturing with bacteria to act as a source of nutrition. Such cultures will contain varying levels of intrinsic bacterial contamination that can interfere with molecular research and cause problems with the collection of sufficient material for sequencing. Measuring the levels of bacterial contamination for ... More
Identification and characterization of non-saccharomyces spoilage yeasts isolated from Brazilian wines.
Authors:Echeverrigaray S, Randon M, da Silva K, Zacaria J, Delamare AP
Journal:World J Microbiol Biotechnol
PubMed ID:23355138
'The industry of fine wines and also locally consumed table wines is emerging in Brazil with an increasing volume and economic impact. Enologists in this region currently lack information about the prevalence and characteristics of spoilage yeasts, which may contaminate and potentially undervalue Brazilian wines. Herein, we analyzed 50 local ... More
Systemically administered gp100 encoding DNA vaccine for melanoma using water-in-oil-in-water multiple emulsion delivery systems.
Authors:Kalariya M, Amiji MM
Journal:
PubMed ID:23702000
'The purpose of this study was to develop a water-in-oil-in-water (W/O/W) multiple emulsions-based vaccine delivery system for plasmid DNA encoding the gp100 peptide antigen for melanoma immunotherapy. The gp100 encoding plasmid DNA was encapsulated in the inner-most aqueous phase of squalane oil containing W/O/W multiple emulsions using a two-step emulsification ... More
Multiplex PCR assay targeting a diguanylate cyclase-encoding gene, cgcA, to differentiate species within the genus Cronobacter.
Authors:Carter L, Lindsey LA, Grim CJ, Sathyamoorthy V, Jarvis KG, Gopinath G, Lee C, Sadowski JA, Trach L, Pava-Ripoll M, McCardell BA, Tall BD, Hu L
Journal:Appl Environ Microbiol
PubMed ID:23144142
In a comparison to the widely used Cronobacter rpoB PCR assay, a highly specific multiplexed PCR assay based on cgcA, a diguanylate cyclase gene, that identified all of the targeted six species among 305 Cronobacter isolates was designed. This assay will be a valuable tool for identifying suspected Cronobacter isolates ... More
Mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) genotyping of mycobacterium intracellulare for strain comparison with establishment of a PCR-based database.
Authors:Iakhiaeva E, McNulty S, Brown Elliott BA, Falkinham JO, Williams MD, Vasireddy R, Wilson RW, Turenne C, Wallace RJ
Journal:J Clin Microbiol
PubMed ID:23175249
Strain comparison is important to population genetics and to evaluate relapses in patients with Mycobacterium avium complex (MAC) lung disease, but the "gold standard" of pulsed-field gel electrophoresis (PFGE) is time-consuming and complex. We used variable-number tandem repeats (VNTR) for fingerprinting of respiratory isolates of M. intracellulare from patients with ... More