100 bp DNA 分子量标准
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100 bp DNA 分子量标准
Invitrogen™

100 bp DNA 分子量标准

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Invitrogen 100 bp DNA 分子量标准设计用于 100 bp 至 2,000 bp了解更多信息
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货号数量
15628050500 μL DNA 分子量标准,1 mL 10X 上样缓冲液
15628019100 μL DNA 分子量标准,1 mL 10X 上样缓冲液,100 次应用
货号 15628050
价格(CNY)
7,939.00
Each
添加至购物车
数量:
500 μL DNA 分子量标准,1 mL 10X 上样缓冲液
请求批量或定制报价
价格(CNY)
7,939.00
Each
添加至购物车
Invitrogen 100 bp DNA 分子量标准设计用于 100 bp 至 2,000 bp 内双链 DNA 片段的大小鉴定和近似定量。100 bp DNA 分子量标准由 13 个层析纯化的个体 DNA 片段组成,参比条带分别为 2000、1500 和 600 bp,便于轻松定向。

100 bp DNA 分子量标准非常适合利用 1–2% 琼脂糖凝胶进行 DNA 片段分离。

100 bp DNA 分子量标准的特点:
明显、清晰的条带—层析纯化片段可获得一致和可靠的结果
便利—已经预混了 10X BlueJuice 凝胶上样缓冲液,便于追踪样品 DNA 迁移
精确—可获得每条带中的 DNA 精确量

产品用途
溴化乙锭或 SYBR Safe 染色后,可在 1–2% 琼脂糖凝胶上观测到双链 DNA 分子量标准。该分子量标准设计有强度均匀的 DNA 条带,便于清楚查看每一条带。每个条带中确切量的 DNA 可实现样品 DNA 近似定量。

该分子量标准可使用 T4 多聚核苷酸激酶或 T4 DNA 聚合酶进行放射性标记。

仅供科研使用。不可用于诊断程序。
规格
最大浓度0.5 μg/μL
凝胶兼容性琼脂糖凝胶
环保功能绿色可持续包装
套装内容500 μL DNA Ladder, 1 mL 10X Loading Buffer
反应次数500应用
产品类型DNA 分子量标准品
数量500 μL DNA 分子量标准,1 mL 10X 上样缓冲液
即用型
上样容量1 mL
运输条件经批准可在室温或干冰上运输
技术层析纯化的个体 DNA 片段
容积(公制)500 µL
凝胶类型琼脂糖
大小范围100 bp-2,000 bp
Unit SizeEach
内容与储存
• 500 µL 100 bp DNA 分子量标准
• 1 mL 10X BlueJuice 凝胶上样缓冲液

储存于 -20°C。

常见问题解答 (FAQ)

为什么我的分子量ladder 的DNA条带出现了弥散现象?

这里是一些可能导致条带弥散的原因:

•DNA被降解。请避免DNA标准品被核酸酶污染。
•凝胶内上样了过多的DNA。减少凝胶内的DNA量。
•DNA被蛋白污染。电泳前使用苯酚抽提除去蛋白。
•对于小分子量DNA,条带可能会在染色时扩散。在电泳之前加入溴化乙锭(EB)。
•对于放射性标记的DNA,标记是使用切口平移进行的。使用T4 DNA聚合酶对DNA进行置换合成来标记,或者使用T4多核苷酸激酶进行5’末端标记。
•使用了不合适的电泳条件。电泳电压不能超过~20 V/cm,电泳时保持温度<30°C,检查电泳缓冲液是否具有足够的缓冲能力。
•DNA含盐太多。电泳前使用乙醇沉淀去除过多的盐。

我的DNA ladder出现异常迁移。这是为什么?

如果marker被加热了,会出现这种情况。请确保DNA ladder在使用前没有被加热过。

TrackIt DNA梯度标准品与其他DNA梯度标准品有何区别?

TrackIt DNA梯度标准品的样品缓冲液中含有2种示踪染料,可作为观察凝胶电泳进程的视觉指示分子,并能指示何时达到最大分辨率。示踪染料不会遮盖梯度标准品中的DNA条带,因为染料会跑出梯度标准品中大部分DNA条带的范围。
TrackIt蓝绿色/橙黄色上样缓冲液的配方含有独特的示踪染料,二甲苯蓝FF和橙黄G。我们推荐将TrackIt蓝绿色/橙黄色上样缓冲液用于10bp至1kb间的DNA片段。
TrackIt蓝绿色/黄色上样缓冲液含有独特的示踪染料,二甲苯蓝FF和柠檬黄。我们推荐将TrackIt蓝绿色/黄色上样缓冲液用于100bp至10kb间的DNA片段。二甲苯蓝FF的分子量为638.6,橙黄G为452.4,柠檬黄为534.4。
备注:不推荐将TrackIt DNA 梯度标准品用于聚丙烯酰氨凝胶,并且不推荐用于定量分析。

Can I know the sequences of Invitrogen DNA ladders?

Sequences of Invitrogen DNA and RNA ladders are proprietary.

Are Invitrogen DNA ladders composed of linear or circular/supercoiled DNA?

Invitrogen DNA ladders contain linear dsDNA fragments.

引用和文献 (6)

引用和文献
Abstract
A duplex PCR-based assay for measuring the amount of bacterial contamination in a nucleic acid extract from a culture of free-living protists.
Authors:Marron AO, Akam M, Walker G
Journal:PLoS One
PubMed ID:23593495
'Cultures of heterotrophic protists often require co-culturing with bacteria to act as a source of nutrition. Such cultures will contain varying levels of intrinsic bacterial contamination that can interfere with molecular research and cause problems with the collection of sufficient material for sequencing. Measuring the levels of bacterial contamination for ... More
Identification and characterization of non-saccharomyces spoilage yeasts isolated from Brazilian wines.
Authors:Echeverrigaray S, Randon M, da Silva K, Zacaria J, Delamare AP
Journal:World J Microbiol Biotechnol
PubMed ID:23355138
'The industry of fine wines and also locally consumed table wines is emerging in Brazil with an increasing volume and economic impact. Enologists in this region currently lack information about the prevalence and characteristics of spoilage yeasts, which may contaminate and potentially undervalue Brazilian wines. Herein, we analyzed 50 local ... More
Systemically administered gp100 encoding DNA vaccine for melanoma using water-in-oil-in-water multiple emulsion delivery systems.
Authors:Kalariya M, Amiji MM
Journal:
PubMed ID:23702000
'The purpose of this study was to develop a water-in-oil-in-water (W/O/W) multiple emulsions-based vaccine delivery system for plasmid DNA encoding the gp100 peptide antigen for melanoma immunotherapy. The gp100 encoding plasmid DNA was encapsulated in the inner-most aqueous phase of squalane oil containing W/O/W multiple emulsions using a two-step emulsification ... More
Multiplex PCR assay targeting a diguanylate cyclase-encoding gene, cgcA, to differentiate species within the genus Cronobacter.
Authors:Carter L, Lindsey LA, Grim CJ, Sathyamoorthy V, Jarvis KG, Gopinath G, Lee C, Sadowski JA, Trach L, Pava-Ripoll M, McCardell BA, Tall BD, Hu L
Journal:Appl Environ Microbiol
PubMed ID:23144142
In a comparison to the widely used Cronobacter rpoB PCR assay, a highly specific multiplexed PCR assay based on cgcA, a diguanylate cyclase gene, that identified all of the targeted six species among 305 Cronobacter isolates was designed. This assay will be a valuable tool for identifying suspected Cronobacter isolates ... More
Mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) genotyping of mycobacterium intracellulare for strain comparison with establishment of a PCR-based database.
Authors:Iakhiaeva E, McNulty S, Brown Elliott BA, Falkinham JO, Williams MD, Vasireddy R, Wilson RW, Turenne C, Wallace RJ
Journal:J Clin Microbiol
PubMed ID:23175249
Strain comparison is important to population genetics and to evaluate relapses in patients with Mycobacterium avium complex (MAC) lung disease, but the "gold standard" of pulsed-field gel electrophoresis (PFGE) is time-consuming and complex. We used variable-number tandem repeats (VNTR) for fingerprinting of respiratory isolates of M. intracellulare from patients with ... More