Thermo Scientific™

Pierce™ 海肾荧光素酶辉光检测试剂盒

Name: Pierce™ 海肾荧光素酶辉光检测试剂盒
SKU: 16166
Price: 975.00 CNY

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货号	数量
16166	100 次反应试剂盒
16167	1000 次反应试剂盒

2 选项

货号 16166

价格（CNY)

975.00

飞享价

Ends: 31-Dec-2026

1,335.00

共减 360.00 (27%)

Each

数量:

100 次反应试剂盒

价格（CNY)

975.00

飞享价

Ends: 31-Dec-2026

1,335.00

共减 360.00 (27%)

Each

Thermo Scientific Pierce 海肾荧光素酶辉光检测试剂盒尤其在绿色海肾荧光素酶报告基因存在的情况下提供极其明亮且稳定的生物发光信号（半衰期大约3 小时）。

海肾荧光素酶辉光检测试剂盒的特点：

•灵敏—对绿色海肾荧光素酶活性进行高度灵敏的检测
• 稳定—信号稳定性比闪光测定更高
• 简单—不需要使用配备进样器的光度计
• 兼容—与其他海肾荧光素酶兼容的测定试剂
• 适合自动化—适合用于高通量测定
• 便利—包含一种通用的细胞裂解缓冲液和经过优化的辉光测定试剂
• 安全—允许用户执行非放射性测定

该 Pierce 荧光素酶辉光检测试剂盒包含的试剂用于测定细胞裂解物中海肾荧光素酶的活性。在荧光素酶存在的情况下，辉光试剂提供稳定的生物发光信号，非常适合不带进样器的光度计或用于样品批量处理。荧光素酶反应产生的光输出可与生成的荧光素酶蛋白的量相关，后者又与驱动荧光素酶表达的启动子的活性成正比。该辉光测定试剂经过了优化，可以为 Thermo Scientific 绿色海肾 Luc 质粒提供较好结果；但是，这种辉光试剂盒可用于检测其他使用腔肠素作为底物的海肾荧光素酶衍生物的活性。

包括：
细胞裂解缓冲液、反应缓冲液和底物

需要：
海肾荧光素酶和光度计或其他能够监测发光的仪器（如 Thermo Scientific Luminoskan Ascent 和 Varioskan 闪光酶标仪）。

应用：
• 用于分析顺式调节元素和反式作用因子的启动子研究
• 药物筛选
• siRNA 和 miRNA 筛选
• 研究脱靶效应的多通路测定
• 蛋白定位报告基因测定
• 信号转导通路分析
• RNA 剪接研究

绿色海肾荧光素酶是来自海肾的野生型海肾荧光素酶基因衍生物产生的一种 36kDa 蛋白。与野生型荧光素酶相比，绿色海肾在血清中更稳定，并且具有一种向绿色区域转移的发射光谱。该蛋白提供快速衰变的极其明亮的闪光信号。Pierce 海肾辉光检测试剂盒试剂可以有效稳定这种明亮的生物发光，产生大于 3 小时的信号半衰期，因此无需采用带有进样器的光度计。该试剂盒经过了优化，可以为 Thermo Scientific 绿色海肾 Luc 质粒提供较好结果；但是，这种辉光试剂盒可用于检测其他使用腔肠素作为底物的海肾荧光素酶衍生物的活性。

相关产品
Pierce™ 海肾萤火虫荧光素酶双检测试剂盒

仅供科研使用。不可用于诊断程序。

绿色海肾荧光素酶是海肾的野生型海肾荧光素酶基因衍生物产生的一种 36kDa 蛋白。与野生型荧光素酶相比，绿色海肾在血清中更稳定，并且具有一种向绿色区域转移的发射光谱。该蛋白提供快速衰变的极其明亮的闪光信号。Pierce 海肾辉光检测试剂盒试剂可以有效稳定这种明亮的生物发光，产生大于 3 小时的信号半衰期，因此无需采用带有进样器的光度计。该试剂盒经过了优化，可以为 Thermo Scientific 绿色海肾 Luc 质粒提供较好结果；但是，这种辉光试剂盒可用于检测其他使用腔肠素作为底物的海肾荧光素酶衍生物的活性。

参考文献：

Lorenz, W.W., et al.(1991).Proc.Natl.Acad.Sci.USA, 88:4438-4442.

规格

检测报告基因酶、荧光素酶报告基因检测

适用细胞哺乳动物细胞

检测方法生物发光

适用于（设备）Luminometer

产品规格96-well Plate, 384-well Plate

标签类型酶标记

产品线Pierce

数量100 次反应试剂盒

底物腔肠素

底物属性化学底物

底物类型荧光素酶底物

靶标荧光素酶、绿色海肾荧光素酶

技术增强的化学发光法

类型检测试剂盒

Unit SizeEach

内容与储存

接收后,在 -80°C 下储存试剂盒或按上文所述储存单一组分。
• 海肾辉光测定缓冲液,5 mL,在 4°C 下储存
• 腔肠素 (100X),50 μL,在 -80°C 下储存
• 2X 细胞裂解缓冲液,6 mL,在室温下储存

常见问题解答 (FAQ)

I got a high background signal with a Gaussia/Cypridina/Renilla Luciferase Glow/Luciferase Flash assay. What went wrong?

Here are possible causes and solutions:

- Non-specific oxidation of substrate: Use less serum in the cell culture media; Note: Albumin can increase the auto-oxidation of Vargulin; Avoid repeated freezing and thawing of the sample.
- Control sample is contaminated: Use new sample; Change pipette tips after each well.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I am getting a high saturating signal with a Gaussia/Cypridina/Renilla Luciferase Glow/Luciferase Flash assay. What could have happened?

This could be due to high luciferase expression. Here are some suggestions:

Reduce incubation time before collecting samples.

Decrease the integration time on the instrument.

Dilute the sample: for secreted Gaussia/Cypridina Luciferase, dilute the sample using media from the cell culture; for cell lysate, dilute the sample using lysis buffer

Note: A low sample volume can increase assay variability. Dilute the sample and use the recommended volume of 10-20 µL per assay.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Why am I getting a low signal in lysate using a Gaussia/Cypridina/Renilla Luciferase Glow/Luciferase Flash assay? Can you provide some suggestions?

Here are possible causes and solutions:

- Non-optimized lysis buffer used: Assay luciferase activity in the media to confirm good expression of luciferase; Use only the provided lysis buffer.
- Low luciferase expression: Lyse cells in smaller volume of 1X Cell Lysis Buffer; Use a different promoter or growth conditions to improve expression; Increase the integration time on the instrument; Scale up volume of sample and reagent per well.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I got a low signal in media when I used a Gaussia/Cypridina/Renilla Luciferase Glow/Luciferase Flash assay. What happened?

Here are possible causes and solutions:

- Insufficient luciferase accumulation in media: Incubate cells for a longer time.
- Low luciferase expression: Use less media per well during the experiment;.Use a different promoter or growth conditions to improve expression;.Increase the integration time on the instrument;.Scale-up the volume of sample and reagent per well.
- Treatment interfered with cellular secretory pathway: Transfect cells with a plasmid for constitutive expression of Luciferase (i.e., with pTK or pCMV promoter); Determine if luciferase actively expresses in media without treatment. Add treatment; Determine if there is a corresponding drop in luciferase activity from the constitutively expressed plasmid.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I am getting no signal with my Gaussia/Cypridina/Renilla Luciferase Glow/Luciferase Flash assay. Why is this?

Here are possible causes and solutions:

- Low transfection efficiency: Optimize transfection conditions using a visual transfection control (e.g., a plasmid over-expressing a fluorescent protein); Verify plasmid DNA quality - use only transfection grade DNA; Use actively dividing, low passage cells; Use a different cell type.
- No or low promoter activity: Use conditions known for promoter activation; Incubate cells for a longer time; Change growth conditions to improve expression; Use a different promoter.
- Substrate auto-oxidized: Protect substrate from light and air; Maintain 100X Coelenterazine at -80 degrees C; maintain 100X Vargulin at -80 degrees C; Prepare new Coelenterazine Working Solution if used longer than 8 hours; Prepare new Vargulin Working Solution if used longer than 2 hours

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Pierce™ 海肾荧光素酶辉光检测试剂盒 FAQs