Pierce™ 萤火虫荧光素酶闪光检测试剂盒
Pierce™ 萤火虫荧光素酶闪光检测试剂盒
Thermo Scientific™

Pierce™ 萤火虫荧光素酶闪光检测试剂盒

Thermo Scientific Pierce 萤火虫荧光素酶闪光检测试剂盒提供了用于测量全细胞内红色萤火虫荧光素酶活性的试剂,可以产生快速衰变的非常强的闪光信号。萤火虫荧光素酶闪光检测试剂盒的特点:•对萤火虫荧光素酶活性进行极灵敏的检测• 与 Pierce 萤火虫荧光素酶信号增强剂配合使用可获得较好结果(较大信号)•了解更多信息
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货号数量
16174100 次反应试剂盒
161751000 次反应试剂盒
货号 16174
价格(CNY)
1,166.00
Each
添加至购物车
数量:
100 次反应试剂盒
价格(CNY)
1,166.00
Each
添加至购物车
Thermo Scientific Pierce 萤火虫荧光素酶闪光检测试剂盒提供了用于测量全细胞内红色萤火虫荧光素酶活性的试剂,可以产生快速衰变的非常强的闪光信号。

萤火虫荧光素酶闪光检测试剂盒的特点:

•对萤火虫荧光素酶活性进行极灵敏的检测
• 与 Pierce 萤火虫荧光素酶信号增强剂配合使用可获得较好结果(较大信号)
• 工作溶液在 -20°C
• 下可稳定保存长达2个月
• 适合自动化
• 包含一种通用的细胞裂解缓冲液和经过优化的闪光测定试剂
• 安全的非放射性测定试剂

Thermo Scientific Pierce 荧光素酶闪光测定试剂盒包含的试剂用于测定哺乳动物全细胞裂解物中萤火虫荧光素酶的活性。该试剂盒包含便利的裂解缓冲液、检测缓冲液和萤火虫底物,提供了用于对启动子或通路活性进行细胞内检测的完整试剂系统。荧光素酶反应产生的光输出将使用光度计进行捕获,可与生成的萤火虫荧光素酶蛋白的量相关,并可用于确定驱动萤火虫表达的启动子的活性。这些试剂经过优化,可与 Thermo Scientific Firefly Luc 质粒配合使用;但是,闪光分析试剂盒可用于检测采用 D-荧光素作为底物的其他萤火虫或 ATP 依赖性荧光素酶的活性。

包括:
细胞裂解缓冲液、反应缓冲液和底物;需单独购买荧光素酶信号增强剂

需要:
• 萤火虫荧光素酶和光度计或其他能够监测发光的仪器(如 Thermo Scientific Luminoskan Ascent 和 Varioskan 闪光微孔板读数仪)。
•为了获得较好结果(较大信号),请与 Pierce 萤火虫荧光素酶信号增强剂配合使用

应用:
• 用于分析顺式调节元素和反式作用因子的启动子研究
• 药物筛选
• siRNA 和 miRNA 筛选
• 用于研究脱靶效应的多通路测定
• 分泌通路/蛋白定位报告基因测定
• 信号转导通路分析
• RNA 剪接研究

萤火虫荧光素酶是由甲虫萤科家族的多个种属(包括 Photinus 属和萤属)天然产生的一种 60kDa 蛋白。萤火虫荧光素酶的生物发光信号源自 D-荧光素的氧化。使用光度计捕获的光输出可与生成的萤火虫荧光素酶蛋白的量相关,并可用于确定驱动萤火虫表达的启动子的活性。

更多产品数据
使用多通路荧光素酶报告基因监测神经元分化
通过农药化学品激活抗氧化反应途径
多功能荧光素酶:微孔板光度计和闪光荧光素酶测定试剂
仅供科研使用。不可用于诊断程序。
规格
检测报告基因酶、荧光素酶报告基因检测
适用细胞哺乳动物细胞
检测方法生物发光
适用于(设备)化学发光酶标仪(微孔板)
产品规格384 孔板、96 孔板
标签类型酶标记
产品线Pierce™
数量100 次反应试剂盒
底物D-荧光素
底物属性化学底物
底物类型荧光素酶底物
靶标荧光素酶、萤火虫荧光素酶
技术增强的化学发光法
类型检测试剂盒
Unit SizeEach
内容与储存
• 萤火虫闪光测定缓冲液,5 mL,在 4°C 下储存
• D-荧光素,冻干,3 mg,在 -4°C 下储存
• 2X 细胞裂解缓冲液,6 mL,在室温下储存

接收后,在 4°C 下储存试剂盒或按上文所述储存单一组分。

常见问题解答 (FAQ)

I got a high background signal with a Firefly Luciferase Glow/Flash assay. Why is this?

This could be due to contamination of the control sample. Make sure to use new sample and change pipette tips after each well.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I got a high signal with a Firefly Luciferase Glow/Flash assay. What could have happened?

This could be due to high luciferase expression. Here are some suggestions:

  • Reduce incubation time before collecting samples. 
  • Decrease the integration time on the instrument.
  • Dilute the sample 
    Note: A low sample volume can increase assay variability. Dilute the sample and use the recommended volume of 10-20 µL per assay



  • Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

    What could be causing no signal or low signal with the Firefly Luciferase Glow/Luciferase Flash assay? Do you have any suggestions to get around this problem?

    Here are possible causes and solutions:

    - Low transfection efficiency: Optimize transfection conditions using a visual transfection control (e.g., a plasmid over-expressing a fluorescent protein); Verify plasmid DNA quality - use only transfection grade DNA; Use actively dividing, low passage cells; Use a different cell type.
    - No or low promoter activity: Use conditions known for promoter activation; Incubate cells for a longer time; Change growth conditions to improve expression; Use a different promoter.
    - D-luciferin auto-oxidized: Protect substrate from light and maintain 100X D-luciferin at -20 degrees C; Prepare new Working Solution if used longer than 4 hours.
    - Low luciferase expression: Use Pierce Firefly Signal Enhancer (100X) (Cat. No. 16180); Lyse cells in a smaller volume of 1X Cell Lysis Buffer; Use a different promoter or growth conditions to improve expression; Increase the integration time on the instrument; Scale-up the volume of sample and reagent per well.
    - Degraded luciferase protein: Store cell lysates on ice and perform assays immediately following cell lysis.

    Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

    I got a high background signal with a Gaussia/Cypridina/Renilla Luciferase Glow/Luciferase Flash assay. What went wrong?

    Here are possible causes and solutions:

    - Non-specific oxidation of substrate: Use less serum in the cell culture media; Note: Albumin can increase the auto-oxidation of Vargulin; Avoid repeated freezing and thawing of the sample.
    - Control sample is contaminated: Use new sample; Change pipette tips after each well.

    Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

    I am getting a high saturating signal with a Gaussia/Cypridina/Renilla Luciferase Glow/Luciferase Flash assay. What could have happened?

    This could be due to high luciferase expression. Here are some suggestions:

  • Reduce incubation time before collecting samples. 
  • Decrease the integration time on the instrument.
  • Dilute the sample: for secreted Gaussia/Cypridina Luciferase, dilute the sample using media from the cell culture; for cell lysate, dilute the sample using lysis buffer 


  • Note: A low sample volume can increase assay variability. Dilute the sample and use the recommended volume of 10-20 µL per assay.

    Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.