用于荧光素酶检测的 pCMV-Gaussia-Dura Luc 载体

Thermo Scientific pCMV-Gaussia-Dura Luc 载体是 pMCS-Gaussia-Dura Luc 的衍生物。它含有一种突变形式的 Gaussia了解更多信息

货号	数量
16191	10 µg

货号 16191

价格（CNY)

1,232.00

添加至购物车

10 µg

价格（CNY)

1,232.00

添加至购物车

Thermo Scientific pCMV-Gaussia-Dura Luc 载体是 pMCS-Gaussia-Dura Luc 的衍生物。它含有一种突变形式的 Gaussia 荧光素酶基因（具有比天然荧光素酶更好的生物发光信号稳定性），该基因受强巨细胞病毒 (CMV) 启动子控制。此组成型表达载体可用作归一化对照品，以结合其他报告基因解释实验变化。

pCMV-Gaussia-Dura Luc 载体的特点：

•用于共转染和归一化的强 (CMV) 组成型启动子
•天然分泌 Gaussia-Dura 荧光素酶基因，经优化可实现哺乳动物系统中的高表达和辉光稳定性
• 多克隆位点 (MCS) 为调控元件从一个质粒转移至另一个质粒提供了多功能性
•用于通过降低转录的通读来最小化背景的转录终止位点 (Ter)、Lac 操纵基因 (Lac O1) 和转录中断位点 (TPS)
• 分别用于哺乳动物和细菌细胞中的药物选择的嘌呤霉素 (Pur) 和氨苄青霉素 (Amp) 标志物
• 高拷贝 pUC 细菌 DNA 复制起点

Gaussia-Dura 荧光素酶（约 20 kDa ）是一种分泌性蛋白，可测量培养基（实时检测）和细胞裂解物中的报告基因活性。亲代 pMCS 载体含有多克隆位点，用于克隆启动子以研究其调控潜力。

这些载体需获得有限使用标记许可。

仅供科研使用。不可用于诊断程序。

克隆方法限制性内切酶/MCS

输送类型转染

描述pCMV-Gaussia-Dura Luc 载体

适用于（应用）报告基因测定

数量10 µg

报告基因Gaussia Dura 荧光素酶

选择试剂（真核生物）嘌呤霉素

促进剂CMV

类型Luc 载体

Unit SizeEach

内容与储存

储存在原容器中，置于干燥、阴凉且通风良好的区域内，避免阳光直照，在以下温度之间：-70 至 -20°C。

常见问题解答 (FAQ)

What options do you offer for luciferase vectors?

There are three vectors available for each expressed luciferase; pMCS, pCMV, and pTK. We recommend using pMCS to measure the activity of the reporter, and pCMV, and pTK as positive control vectors. For high level constitutive expression of Luciferase, we recommend using the CMV (Cytomegalovirus) promoter vector and for low level constitutive expression of luciferase, we recommend using the pTK (thymidine kinase) promoter vector.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the stability of the secreted luciferase?

Gaussia, Gaussia-Dura, and Cypridina Luciferase are stable in the culture media for greater than 24 hours. Generally, after transfection of CMV driven constructs, we see ever increasing secreted signal between 24 and 72 hours.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How quickly is luciferase secreted?

The earliest detectable signal will depend on the strength of the promoter. In general, with a strong promoter (such as CMV), significant signal over background can be seen in as little as 20 minutes. However, with a weaker inducible promoter, significant signal over background may take 1-2 hours after induction.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do you still offer quantitative IP (qIP) Luciferase assay kits?

Our qIP Luciferase assay kits have been discontinued but we do carry epitope-tagged (HA or c-Myc) and Tluc (TurboLuc luciferase)-tagged mammalian expression vectors, and qIP protein interaction assay reagents for these assay kits.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.