FreeStyle™ MAX 试剂
FreeStyle™ MAX 试剂
Actual product may vary
引用和文献 (1)
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FreeStyle™ MAX 试剂

FreeStyle™ MAX 试剂是一种专有的非动物源性制剂，用于将质粒 DNA 转染到真核细胞中，其可轻松扩大以生产大量重组蛋白。FreeStyle™ MAX 试剂可在生物生产应用中获得极高表达水平和转染率，细胞毒性较低，并经过特殊配制可与以下产品配合使用：•无血清 FreeStyle™ 293了解更多信息
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货号数量
1644775010 x 15 mL
164471001 mL
1644750015 mL
货号 16447750
价格（CNY)
1,446,330.00
Each
添加至购物车
数量:
10 x 15 mL
价格（CNY)
1,446,330.00
Each
添加至购物车
FreeStyle™ MAX 试剂是一种专有的非动物源性制剂，用于将质粒 DNA 转染到真核细胞中，其可轻松扩大以生产大量重组蛋白。FreeStyle™ MAX 试剂可在生物生产应用中获得极高表达水平和转染率，细胞毒性较低，并经过特殊配制可与以下产品配合使用：

•无血清 FreeStyle™ 293 表达培养基（货号 12338-018）中的 FreeStyle™ 293-F 细胞（悬浮人胚肾细胞，货号 R790-07）
•无血清 FreeStyle™ CHO 表达培养基（货号 12651-014）中的 FreeStyle™ CHO-S 细胞（悬浮中国仓鼠卵巢细胞，货号 R800-07）
•含 8 mM L-谷氨酰胺和 18 mL/L 10% Pluronic™ F-68 的 CD DG44 培养基（货号 A11000-01）中的 DG44 细胞（DHFR– 悬浮 CHO 细胞）

产品用途
FreeStyle™ MAX 试剂预期用于与 FreeStyle™ MAX 293 表达系统（货号 K9000-10）和 FreeStyle™ MAX CHO 表达系统（货号 K9000-20）配合使用。
仅供科研使用。不可用于诊断程序。
规格
分类无动物来源
适用于（应用）蛋白表达
高通量能力不兼容高通量应用（手动）
产品线Freestyle
产品类型转染试剂
数量10 x 15 mL
血清兼容性是
运输条件湿冰
细胞类型已建立的细胞系
产品规格管装
样品类型质粒 DNA
转染技术脂质体介导转染
Unit SizeEach
内容与储存
每个试剂盒包含 10 个单元。每单元包含一小瓶试剂。储存在 +4°C 下。妥善储存时，保证 FreeStyle™ MAX 稳定储存 6 个月。

常见问题解答 (FAQ)

FreeStyle Max试剂相对于293fectin转染试剂的优势是什么？

FreeStyle Max试剂与293fectin转染试剂能够提供水平相近的高转染效率；不过，FreeStyle Max试剂具有更低的细胞毒性，进而能够实现更高的蛋白得率。此外，FreeStyle Max试剂为非动物源性的。  

培养于FreeStyle 293表达培养基中的FreeStyle 293-F细胞能使用ExpiFectamine293试剂和增强剂进行转染吗？如果可以，转染效率会比使用FreeStyle Max试剂更高吗？

您可使用ExpiFectamine293试剂来转染FreeStyle 293表达培养基中培养的FreeStyle 293-F细胞；不过，增强剂只会对蛋白表达提供少许的促进作用，因为这一产品是针对更高密度培养设计的，而FreeStyle 293表达培养基并不适用。 

What is the advantage of FreeStyle Max Reagent over 293fectin Transfection Reagent?

Both FreeStyle Max Reagent and 293fectin Transfection Reagent provide similar levels of high transfection efficiency; however, FreeStyle Max Reagent has lower cytotoxicity and hence results in higher protein yields. Additionally, FreeStyle Max Reagent is animal-origin free.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can FreeStyle 293-F cells grown in FreeStyle 293 expression medium be transfected with ExpiFectamine293 Reagent along with enhancers? Is so, will the transfection efficiency be higher than that with FreeStyle Max Reagent?

You should be able to use the ExpiFectamine293 Reagent for transfection of Freestyle 293-F cells grown in FreeStyle 293 Expression Medium; however, the enhancers will provide only a little boost in expression as they are designed to work with higher density cultures that FreeStyle 293 Expression Medium cannot support.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What is the recommended ratio of DNA and transfection reagent when transfecting Freestyle cells?

The optimal amount will vary, but we recommend starting with a 1:1 ratio of DNA and lipid. For example, 1.25 ug DNA and 1.25 ul of the reagent for one ml of suspension cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

View all FreeStyle™ MAX试剂 FAQs

引用和文献 (1)

引用和文献
Abstract
Transient transfection factors for high-level recombinant protein production in suspension cultured mammalian cells.
Authors:Liu C, Dalby B, Chen W, Kilzer JM, Chiou HC,
Journal:Mol Biotechnol
PubMed ID:18327552
'The efficient transfection of cloned genes into mammalian cells system plays a critical role in the production of large quantities of recombinant proteins (r-proteins). In order to establish a simple and scaleable transient protein production system, we have used a cationic lipid-based transfection reagent-FreeStyle MAX to study transient transfection in ... More