II 型胶原酶，粉末
II 型胶原酶，粉末
引用和文献 (2)
Gibco™

II 型胶原酶，粉末

胶原酶是一种蛋白酶，其可以切割 Pro-X-Glyc-Pro 序列（常见于胶原蛋白）中的中性氨基酸 (X) 与甘氨酸之间的键。在蛋白酶中，只有胶原酶能够降解常见于皮肤、肌腱、血管和骨骼等结缔组织的三螺旋天然胶原纤维了解更多信息
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货号数量
171010151 g
货号 17101015
价格（CNY)
2,403.55
飞享价
Ends: 31-Dec-2026
5,253.00
共减 2,849.45 (54%)
Each
添加至购物车
数量:
1 g
价格（CNY)
2,403.55
飞享价
Ends: 31-Dec-2026
5,253.00
共减 2,849.45 (54%)
Each
添加至购物车
胶原酶是一种蛋白酶，其可以切割 Pro-X-Glyc-Pro 序列（常见于胶原蛋白）中的中性氨基酸 (X) 与甘氨酸之间的键。在蛋白酶中，只有胶原酶能够降解常见于皮肤、肌腱、血管和骨骼等结缔组织的三螺旋天然胶原纤维。胶原酶解聚适用于人类肿瘤、小鼠肾脏、成人和胎儿大脑以及许多其他组织（包括上皮细胞）的培养。胶原酶相对温和，在生理温度和 pH 值条件下具有良好的解离能力，无需机械搅拌或特殊设备。

Gibco™ II 型胶原酶分离自溶组织梭菌，包装形式为冻干非无菌粉末，供细胞或组织解离以及器官灌注等研究使用。我们保证 Gibco™ II 型胶原酶的活性大于 125 单位/mg。与其他胶原酶制剂相比，Gibco™ II 型胶原酶的梭菌蛋白酶活性较高，非常适合消化心脏、骨、甲状腺、软骨和肝脏组织。
仅供科研使用。不可用于诊断程序。
规格
数量1 g
有效期24 个月
运输条件室温
形式冻干
产品类型胶原酶
无菌未灭菌
Unit SizeEach
内容与储存
储存条件：2°C 至 8°C。避光储存。
运输条件：室温
有效期：自生产之日起 24 个月

常见问题解答 (FAQ)

你们提供哪些用于解离细胞的试剂？这些产品之间的主要区别是什么？

请通过此选择列表(https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html)来比较我们的细胞解离试剂。

Can I reconstitute Collagenase, Type II, powder (Cat. No. 17101015) into DMEM or MEM alpha instead of HBSS with Ca and Mg?

Yes, Collagenase, Type II, powder can be reconstituted in DMEM or MEM alpha as long as the Mg and Ca concentrations in the medium are similar.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I make the 1000X stock (100 U/µL) solution from Collagenase powder?

1. Add 1 mL Hanks' Balanced Salt Solution (HBSS) with calcium and magnesium directly to 1 g vial of Collagenase. Vortex gently to ensure complete dissolution. Transfer to a clean tube.
2. Determine volume of HBSS (with calcium and magnesium) required to bring collagenase solution to 100 U/µL (1000X stock solution). The activity is lot- specific. Rinse vial with this volume of HBSS (with calcium and magnesium), and combine. Filter sterilize 1000X stock solution with a low protein binding filtration unit.
Example: Assuming the lot you have purchased has an activity of 265 U/mg, this lot will have 265000 Units per mL when you reconstitute collagenase into HBSS (with calcium and magnesium) at 1 g/mL. In order to dilute 265000 U/L to 100000 U/mL (= 100 U/µL), you need to dilute the 1 g/mL enzyme solution 2.65 fold.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

引用和文献 (2)

引用和文献
Abstract
Isolation and in vitro culture of primary cardiomyocytes from adult zebrafish hearts.
Authors:Sander V, Suñe G, Jopling C, Morera C, Izpisua Belmonte JC,
Journal:Nat Protoc
PubMed ID:23538883
'This protocol describes how to isolate primary cardiomyocytes from adult zebrafish hearts and culture them for up to 4 weeks, thereby using them as an alternative to in vivo experiments. After collagenase digestion of the ventricle, cells are exposed to increasing calcium concentrations in order to obtain high-purity cardiomyocytes. The ... More
The PDZ binding motif of the Beta 1 adrenergic receptor modulates receptor trafficking and signaling in cardiac myocytes.
Authors: Xiang Yang; Devic Eric; Kobilka Brian;
Journal:J Biol Chem
PubMed ID:12097326
'beta(1) and beta(2) adrenergic receptors (AR) regulate the intrinsic contraction rate in neonatal mouse cardiac myocytes through distinct signaling pathways. It has been shown that stimulation of beta(1)ARs leads to a protein kinase A-dependent increase in contraction rate. In contrast, stimulation of beta(2)ARs has a biphasic effect on contraction rate, ... More