尿嘧啶 DNA 糖基化酶

引用和文献 (3)

Invitrogen™

尿嘧啶 DNA 糖基化酶

尿嘧啶 DNA 糖基化酶（尿嘧啶-N-糖基化酶）可从单链和双链 DNA 的糖基部分除去尿嘧啶残基，同时又不破坏磷酸二酯主链，从而防止其成为杂交靶标或 DNA 聚合酶的模板。UDG了解更多信息

货号	数量
18054015	100 units

货号 18054015

价格（CNY)

2,341.00

添加至购物车

100 units

价格（CNY)

2,341.00

添加至购物车

尿嘧啶 DNA 糖基化酶（尿嘧啶-N-糖基化酶）可从单链和双链 DNA 的糖基部分除去尿嘧啶残基，同时又不破坏磷酸二酯主链，从而防止其成为杂交靶标或 DNA 聚合酶的模板。UDG 不会从 RNA 中去除尿嘧啶。

应用：有助于消除 PCR 中的残留污染，从而减少克隆 PCR 片段 (3) 的假阳性结果 (1,2)。

来源：纯化自质粒上表达大肠杆菌 UNG 基因的大肠杆菌 (4,5)。

性能和质量测试：核酸外切酶、核酸内切酶和磷酸酶分析。

单位定义：在 37°C 条件下，一单位可在 1 小时内，催化 ³H-poly (dU) 释放1 nmol 的游离尿嘧啶。

单位反应条件：30 mM Tris-HCl (pH 8.3)、50 mM KCl、5 mM MgCl₂、4 μg/mL ³H-poly（dU）n (7 x 10 5 cpm/μg)、以及1单位
酶 (50 μl) 在37°C 下反应10分钟 (4)。

仅供科研使用。不可用于诊断程序。

聚合酶DNA 聚合酶

数量100 units

运输条件经批准可置于湿冰或干冰上运输

适用于（应用）PCR、实时荧光定量 PCR (qPCR), Real Time PCR (qPCR)

PCR 方法qPCR

Unit SizeEach

内容与储存

在冰箱中储存（ -5° 至 -30°C ）。

常见问题解答 (FAQ)

What is UDG?

UDG (uracil-DNA-glycosylase) refers to a superfamily of enzymes comprising six sub-families. Family I UDG enzymes are called UNG, after the uracil-N-glycosylase gene. The terms UDG and UNG are commonly used interchangeably because they perform the same function in qPCR, namely, to remove Uracil from dU-containing DNA to prevent carryover contamination from previous PCRs. See the following link: https://www.thermofisher.com/uk/en/home/life-science/pcr/real-time-pcr/real-time-pcr-learning-center/real-time-pcr-basics/what-is-ung-udg.html

引用和文献 (3)

引用和文献

Abstract

A novel MHC class I-like gene is mutated in patients with hereditary haemochromatosis.

Authors:Feder JN, Gnirke A, Thomas W, Tsuchihashi Z, Ruddy DA, Basava A, Dormishian F, Domingo R Jr, Ellis MC, Fullan A, Hinton LM, Jones NL, Kimmel BE, Kronmal GS, Lauer P, Lee VK, Loeb DB, Mapa FA, McClelland E, Meyer NC, Mintier GA, Moeller N, Moore T, Morikang E, Wolff RK, et al.

Journal:Nat Genet

PubMed ID:10545944

Hereditary haemochromatosis (HH), which affects some 1 in 400 and has an estimated carrier frequency of 1 in 10 individuals of Northern European descent, results in multi-organ dysfunction caused by increased iron deposition, and is treatable if detected early. Using linkage-disequilibrium and full haplotype analysis, we have identified a 250-kilobase ... More

Structural determinants for HIV-1 integrase inhibition by beta-diketo acids.

Authors: Marchand Christophe; Zhang Xuechun; Pais Godwin C G; Cowansage Kiriana; Neamati Nouri; Burke Terrence R Jr; Pommier Yves;

Journal:J Biol Chem

PubMed ID:11805103

Among all the HIV-1 integrase inhibitors, the beta-diketo acids (DKAs) represent a major lead in anti-HIV-1 integrase drug design. These derivatives inhibit the integration reaction in vitro with a strong specificity for the 3'-end joining step. They are also antiviral and inhibit integration in vivo. The aim of the present ... More

Escherichia coli apurinic-apyrimidinic endonucleases enhance the turnover of the adenine glycosylase MutY with G:A substrates.

Authors: Pope Mary Ann; Porello Silvia L; David Sheila S;

Journal:J Biol Chem

PubMed ID:11960995

The DNA repair enzyme MutY plays an important role in the prevention of DNA mutations resulting from the presence of the oxidatively damaged lesion 7,8-dihydro-8-oxo-2'-deoxyguanosine (OG). MutY is a base excision repair (BER) glycosylase that removes misincorporated adenine residues from OG:A mispairs, as well as G:A and C:A mispairs. We ... More