DNase I，扩增级
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DNase I，扩增级
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DNase I，扩增级

DNase I（扩增级）将单链和双链 DNA 酶切成含 5' 磷酸的寡脱氧核糖核苷酸。DNase I了解更多信息
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货号数量
18068015100 U
货号 18068015
价格（CNY)
1,007.00
飞享价
Ends: 31-Dec-2025
2,275.00
共减 1,268.00 (56%)
Each
添加至购物车
数量:
100 U
请求批量或定制报价
价格（CNY)
1,007.00
飞享价
Ends: 31-Dec-2025
2,275.00
共减 1,268.00 (56%)
Each
添加至购物车
DNase I（扩增级）将单链和双链 DNA 酶切成含 5' 磷酸的寡脱氧核糖核苷酸。DNase I（扩增级）适用于在关键 RNA 净化程序（如 RNA-PCR 扩增前的程序）中消除 DNA。经纯化和检测，RNase 污染水平无法检出。通过使用 RNA 分子量标准品进行核糖核酸酶检测确定无 RNase。

应用
从 RNA 和蛋白制备物中去除 DNA。

比活度
比活度 > 10,000 单位/mg。

来源
从牛胰腺中纯化而来

性能和质量检测
确定使用 RNA 分子量标准品进行核糖核酸酶检测以及将单链和双链 DNA 酶切成寡核苷酸的能力。

单位定义
一单位酶量可在 25°C 下以 0.001 A260 单位/min./mL 反应混合物的速率增加高分子量 DNA 溶液的吸光度。

单位反应条件
0.1 M 醋酸钠（pH 值 5.0）、5 mM MgCl2、50 µg/mL 小牛胸腺 DNA 和 1 mL 酶，25°C 下 10 min。
仅供科研使用。不可用于诊断程序。
规格
兼容缓冲液反应缓冲液
数量100 U
运输条件经批准可置于湿冰或干冰上运输
酶DNase
Unit SizeEach
内容与储存
内容物：
1 样品瓶 DNase I，扩增级 (100 U)
1 样品瓶 10X DNase I 反应缓冲液 (1000 µL)
1 样品瓶 25 mM EDTA（pH 值 8.0）(200 µL)

储存在 -20°C 非自动除霜冷冻冰箱中。

常见问题解答 (FAQ)

如何处理RNA才能去除残留的DNA？

采用不含RNase的DNase处理RNA可以将DNA的含量控制在检测不到的水平。我们建议使用我们的扩增级产品DNase 1（货号18068015）。

How can RNA be treated to remove residual DNA?

RNAse-free DNase treatment of the RNA can reduce DNA to undetectable levels. We recommend using our DNase 1, Amplification Grade (Cat. No. 18068015).

Does Thermo Fisher Scientific offer a protease-free DNase?

We do not test for protease activity as part of our QC but there is PMSF (a protease inhibitor) in the storage buffer. Furthermore, in the preparation of DNase I, we use a soybean trypsin inhibitor column to remove proteases.

How should I treat my RNA sample prior to RT-PCR to ensure that I have no DNA contamination?

DNase I treatment is optional, and one has to consider individual experimental design.

Potential disadvantage of omitting the DNase I step: you may get amplification from genomic DNA. If you omit this step, you will need to include a no RT control and design primers that will not amplify genomic DNA, like those spanning two different exons or exon-exon junctions.

Potential benefit of omitting the DNAse I Step:
saves time; consumes less reagent, saves pipetting steps, and reduces RNA loss (important for precious samples).

Protocol for DNAse I treatment:
Combine 1 µg total RNA, 1 µL 10X DNAse I buffer (200 mM Tris-HCl (pH 8.4), 500 mM KCl, 20 mM MgCl2), 1 µL Amplification Grade DNAse I (1 unit/µL), and DEPC-treated water to 10 µL. Incubate for 15 min at room temperature. Inactivate by adding 1 µL of 25 mM EDTA and heat for 10 min at 65 degrees C.
Note: 1 U of DNAse I should be enough to treat up to ~10 µg of RNA.

To locate the manual for Amplification Grade DNAse I, search www.thermofisher.com with the Cat. No.18068-015. The manual will be one of the links on the product page.

What is the difference between DNase I and Amplification Grade DNase I?

The Amplification Grade DNase I (Cat. No. 18068-015) is subjected to an extra final HPLC purification step to remove traces of RNases. The Amplification Grade DNase I is supplied as 1 unit/µL and comes with 10X buffer (200 mM Tris-HCL pH 8.4, 20 mM MgCl2, 500 mM KCl) and a vial of 25mM EDTA.

In RT-PCR, a large excess of Amplification Grade DNase I could be used to digest an RNA template without degradation of the RNA (in-house data). Use Amplification Grade DNase I to remove genomic DNA carryover in RNA samples prior to RT-PCR.

The regular DNase I is supplied at 5-15 mg/mL (50-375 U/µL) and does not come with its own buffer.

View all DNase I，扩增级 FAQs