Random Primers DNA Labeling System
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Invitrogen™

Random Primers DNA Labeling System

随机引物 DNA 标记系统适用于放射性标记 DNA,特别是片段 ––在一次反应中使用 [α-32 P]-dCTP–25 ng DNA 获得了解更多信息
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货号数量
1818701330 次反应
货号 18187013
价格(CNY)
6,415.00
Each
添加至购物车
数量:
30 次反应
价格(CNY)
6,415.00
Each
添加至购物车
随机引物 DNA 标记系统适用于放射性标记 DNA,特别是片段 <1 kb。 随机引物 DNA 标记系统:
––在一次反应中使用 [α-32 P]-dCTP
–25 ng DNA 获得 >109 cpm/μg 对照 DNA


性能和质量检测:放射性标记核苷酸的掺入可在随机引物标记反应中使用对照 DNA 进行验证。
仅供科研使用。不可用于诊断程序。
规格
检测方法放射性
最终产品类型探针(标记 DNA)
包括标签或染料
标记方法直接标记
标记目标DNA(一般)
标签或染料32P(磷-32)
产品类型DNA 标记系统
数量30 次反应
运输条件经批准可置于湿冰或干冰上运输
Unit SizeEach
内容与储存
随机引物 DNA 标记系统的组分列于右侧表格中。 保存于 -20°C。

常见问题解答 (FAQ)

How can I label COT-1 DNA?

Probes can be labeled with 32P by random primer or nick translation procedures using the Random Primers DNA Labeling System (Cat. No.18187-013) or Nick Translation System (Cat. No. 18160-010). Biotinylated COT-1 DNA can be prepared by nick translation with the BioNick Labeling System (Cat. No. 18247-015) or by the BioPrime DNA Labeling System (Cat. No. 18094-011). Improved results can be obtained when the COT-1 DNA is first ligated to itself to provide an optimum template.

引用和文献 (12)

引用和文献
Abstract
Human biliverdin reductase is a leucine zipper-like DNA-binding protein and functions in transcriptional activation of heme oxygenase-1 by oxidative stress.
Authors:Ahmad Zulfiqar; Salim Mohammad; Maines Mahin D;
Journal:J Biol Chem
PubMed ID:11773068
Human biliverdin reductase (hBVR) is a serine/threonine kinase that catalyzes reduction of the heme oxygenase (HO) activity product, biliverdin, to bilirubin. A domain of biliverdin reductase (BVR) has primary structural features that resemble leucine zipper proteins. A heptad repeat of five leucines (L(1)--L(5)), a basic domain, and a conserved alanine ... More
Activation of the androgen receptor N-terminal domain by interleukin-6 via MAPK and STAT3 signal transduction pathways.
Authors: Ueda Takeshi; Bruchovsky Nicholas; Sadar Marianne D;
Journal:J Biol Chem
PubMed ID:11751884
'The androgen receptor (AR) is a ligand-activated transcription factor that mediates the biological responses of androgens. However, non-androgenic pathways have also been shown to activate the AR. The mechanism of cross-talk between the interleukin-6 (IL-6) and AR signal transduction pathways was investigated in LNCaP human prostate cancer cells. IL-6 induced ... More
PPARgamma knockdown by engineered transcription factors: exogenous PPARgamma2 but not PPARgamma1 reactivates adipogenesis.
Authors: Ren Delin; Collingwood Trevor N; Rebar Edward J; Wolffe Alan P; Camp Heidi S;
Journal:Genes Dev
PubMed ID:11782439
'To determine functional differences between the two splice variants of PPARgamma (gamma1 and gamma2), we sought to selectively repress gamma2 expression by targeting engineered zinc finger repressor proteins (ZFPs) to the gamma2-specific promoter, P2. In 3T3-L1 cells, expression of ZFP55 resulted in >50% reduction in gamma2 expression but had no ... More
Interaction of the Poliovirus Receptor CD155 with the Dynein Light Chain Tctex-1 and Its Implication for Poliovirus Pathogenesis.
Authors: Mueller Steffen; Cao Xuemei; Welker Reinhold; Wimmer Eckard;
Journal:J Biol Chem
PubMed ID:11751937
'The cellular receptor for poliovirus CD155 (or PVR) is the founding member of a new class of membrane-associated immunoglobulin-like proteins, which include the mouse tumor-associated antigen E4 (Tage4) and three proteins termed ' ... More
A trypanosome mitochondrial RNA polymerase is required for transcription and replication.
Authors: Grams Jayleen; Morris James C; Drew Mark E; Wang Zefeng; Englund Paul T; Hajduk Stephen L;
Journal:J Biol Chem
PubMed ID:11859084
'Understanding mitochondrial transcription is a requisite first step toward understanding the regulation of mitochondrial gene expression in kinetoplastids. Here we report the identification and functional characterization of a mitochondrial RNA polymerase (mtRNAP) from Trypanosoma brucei, the first trans-acting factor involved in kinetoplast mitochondrial transcription to be identified. Using sequences conserved ... More