BioNick™ DNA 标记系统

引用和文献 (4)

Invitrogen™

BioNick™ DNA 标记系统

BioNick™ DNA 标记系统是通过针对无放射性原位杂交用途进行优化的切口平移生成生物素化 DNA 探针的理想选择。这些探针也可用于 Southern 或 Northern 印迹了解更多信息

货号	数量
18247015	50 次反应

货号 18247015

价格（CNY)

7,758.00

添加至购物车

50 次反应

价格（CNY)

7,758.00

添加至购物车

BioNick™ DNA 标记系统是通过针对无放射性原位杂交用途进行优化的切口平移生成生物素化 DNA 探针的理想选择。这些探针也可用于 Southern 或 Northern 印迹、斑块升降、菌落杂交和斑点印迹杂交。使用 BioNick™ DNA 标记系统：

•使用生物素-14-dATP 标记 DNA，产生的探针大小为 50 至 500 bp
• 一次反应标记 1 μg 模板 DNA

仅供科研使用。不可用于诊断程序。

包括标签或染料是

标记方法直接标记

产品线BioNick

产品类型DNA 标记系统

数量50 次反应

运输条件经批准可置于湿冰或干冰上运输

检测方法基于生物素

最终产品类型探针(标记 DNA)

标记目标DNA(通用)

标签或染料生物素

Unit SizeEach

内容与储存

BioNick™ DNA 标记系统包括 10X dNTP 混合物(含有生物素-14-dATP、10X 酶混合物(含 DNA 聚合酶 I 和 DNase I)、对照 DNA、终止缓冲液和蒸馏水。储存在 -20°C 下。如果正确储存,可保证稳定保存6个月。

常见问题解答 (FAQ)

What is the difference between the BioNick system and the BioPrime system?

The BioNick Labeling System generates biotinylated DNA probes by nick translation. These probes range from 50 to 500 bases. One reaction labels 1 µg of DNA. The BioPrime Labeling System generates 50-500 ng of biotinylated DNA from 25 ng template DNA by random priming. The probe size ranges from 50 to 700 bases, with a significant portion of the probe being less than 200 bases. See Mackey, J., Rashtchian, A. (1992) FOCUS 14, p.21.

引用和文献 (4)

引用和文献

Abstract

Loss of the SKI proto-oncogene in individuals affected with 1p36 deletion syndrome is predicted by strain-dependent defects in Ski-/- mice.

Authors: Colmenares Clemencia; Heilstedt Heidi A; Shaffer Lisa G; Schwartz Stuart; Berk Michael; Murray Jeffrey C; Stavnezer Ed;

Journal:Nat Genet

PubMed ID:11731796

'Experiments involving overexpression of Ski have suggested that this gene is involved in neural tube development and muscle differentiation. In agreement with these findings, Ski-/- mice display a cranial neural tube defect that results in exencephaly and a marked reduction in skeletal muscle mass. Here we show that the penetrance ... More

Cloning and characterization of a family of proteins associated with Mpl.

Authors: Meunier Caroline; Bordereaux Didier; Porteu Francoise; Gisselbrecht Sylvie; ChrÃ©tien Stany; Courtois GeneviÃ¨ve;

Journal:J Biol Chem

PubMed ID:11784712

'Thrombopoietin (TPO) controls the formation of megakaryocytes and platelets from hematopoietic stem cells via activation of the c-Mpl receptor and multiple downstream signal transduction pathways. We used two-hybrid screening to identify new proteins that interacted with the cytoplasmic domain of Mpl, and we found a new family of proteins designated ... More

Endothelial induction of fgl2 contributes to thrombosis during acute vascular xenograft rejection.

Authors:Ghanekar A, Mendicino M, Liu H, He W, Liu M, Zhong R, Phillips MJ, Levy GA, Grant DR,

Journal:J Immunol

PubMed ID:15100314

Thrombosis is a prominent feature of acute vascular rejection (AVR), the current barrier to survival of pig-to-primate xenografts. Fibrinogen-like protein 2 (fgl2/fibroleukin) is an inducible prothrombinase that plays an important role in the pathogenesis of fibrin deposition during viral hepatitis and cytokine-induced fetal loss. We hypothesized that induction of fgl2 ... More

Structural and functional genomics of the CPT1B gene for muscle-type carnitine palmitoyltransferase I in mammals.

Authors: van der Leij Feike R; Cox Keith B; Jackson Vicky N; Huijkman Nicolette C A; Bartelds Beatrijs; Kuipers Jaap R G; Dijkhuizen Trijnie; Terpstra Peter; Wood Philip A; Zammit Victor A; Price Nigel T;

Journal:J Biol Chem

PubMed ID:12015320

Muscle-type carnitine palmitoyltransferase I (M-CPT I) is a key enzyme in the control of beta-oxidation of long-chain fatty acids in the heart and skeletal muscle. Because knowledge of the mammalian genes encoding M-CPT I may aid in studies of disturbed energy metabolism, we obtained new genomic and cDNA data for ... More