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引用和文献 (6)
Invitrogen™
RadPrime DNA 标记系统
The RadPrime DNA Labeling System is ideal for rapid preparation (under 10 min) of high-specific activity 32 P-labeled probes for了解更多信息
| 货号 | 数量 |
|---|---|
| 18428011 | 30 次反应 |
货号 18428011
价格（CNY)
6,354.00
Each
数量:
30 次反应
价格（CNY)
6,354.00
Each
The RadPrime DNA Labeling System is ideal for rapid preparation (under 10 min) of high-specific activity 32 P-labeled probes for the detection of DNA and RNA (1). The RadPrime DNA Labeling System:
• Produces probes that can detect nucleic acids on northern or Southern blots, plaque lifts, colony hybridizations, and in situ hybridization
• Yields >109 cpm/μg control DNA using [32P]-dCTP
• Labels 25 ng of DNA in one reaction
Performance and Quality Testing: Incorporation of a radioactively labeled nucleotide is verified using control DNA in a RadPrime labeling reaction.
• Produces probes that can detect nucleic acids on northern or Southern blots, plaque lifts, colony hybridizations, and in situ hybridization
• Yields >109 cpm/μg control DNA using [32P]-dCTP
• Labels 25 ng of DNA in one reaction
Performance and Quality Testing: Incorporation of a radioactively labeled nucleotide is verified using control DNA in a RadPrime labeling reaction.
仅供科研使用。不可用于诊断程序。
规格
检测方法放射性
最终产品类型探针（标记 DNA）
包括标签或染料否
标记方法直接标记
标记目标DNA（一般）
标签或染料32P（磷-32）
产品类型DNA 标记系统
数量30 次反应
运输条件干冰
Unit SizeEach
内容与储存
RadPrime DNA 标记系统的组分列于右侧表格中。储存在 -20°C 下。
常见问题解答 (FAQ)
How long does the labeling take when using the RadPrime DNA Labeling system?
引用和文献 (6)
引用和文献
Abstract
Molecular characterization of the homo-phytochelatin synthase of soybean Glycine max: relation to phytochelatin synthase.
Journal:J Biol Chem
PubMed ID:11706029
'The phytochelatin homologs homo-phytochelatins are heavy metal-binding peptides present in many legumes. To study the biosynthesis of these compounds, we have isolated and functionally expressed a cDNA GmhPCS1 encoding homo-phytochelatin synthase from Glycine max, a plant known to accumulate homo-phytochelatins rather than phytochelatins upon the exposure to heavy metals. The
Two Proteins Essential for Apolipoprotein B mRNA Editing Are Expressed from a Single Gene through Alternative Splicing.
Journal:J Biol Chem
PubMed ID:11815617
'Apolipoprotein B (apoB) mRNA editing involves site-specific deamination of cytidine to form uridine, resulting in the production of an in-frame stop codon. Protein translated from edited mRNA is associated with a reduced risk of atherosclerosis, and hence the protein factors that regulate hepatic apoB mRNA editing are of interest. A
Activation of the phosphatidylinositol 3-kinase/Akt signaling pathway by retinoic acid is required for neural differentiation of SH-SY5Y human neuroblastoma cells.
Journal:J Biol Chem
PubMed ID:12000752
'Retinoic acid (RA) induces neural differentiation of SH-SY5Y neuroblastoma cells. We show that the mRNA levels of the differentiation-inhibiting basic helix-loop-helix transcription factors ID1, ID2, and ID3 are down-regulated during RA-induced differentiation of SH-SY5Y cells. The levels of ID proteins decreased in parallel to the observed transcriptional repression. The expression
Novel Alternative Splicings of BPAG1 (Bullous Pemphigoid Antigen 1) Including the Domain Structure Closely Related to MACF (Microtubule Actin Cross-linking Factor).
Journal:J Biol Chem
PubMed ID:11751855
'BPAG1 (bullous pemphigoid antigen 1) was originally identified as a 230-kDa hemidesmosomal protein and belongs to the plakin family, because it consists of a plakin domain, a coiled-coil rod domain and a COOH-terminal intermediate filament binding domain. To date, alternatively spliced products of BPAG1, BPAG1e, and BPAG1n are known. BPAG1e
A DEAD-Box Protein Functions as an ATP-Dependent RNA Chaperone in Group I Intron Splicing.
Journal:Cell
PubMed ID:12086675
The Neurospora crassa CYT-18 protein, the mitochondrial tyrosyl-tRNA synthetase, functions in splicing group I introns by inducing formation of the catalytically active RNA structure. Here, we identified a DEAD-box protein (CYT-19) that functions in concert with CYT-18 to promote group I intron splicing in vivo and vitro. CYT-19 does not