MagnaBind™ 链霉素亲和素微珠
MagnaBind™ 链霉素亲和素微珠
Thermo Scientific™

MagnaBind™ 链霉素亲和素微珠

Thermo Scientific MagnaBind 链霉素亲和素微珠方便用于涉及生物素标记分子的亲和纯化或分离方法。为从悬浮液中取出 MagnaBind 微珠,请使用外部磁场。MagnaBind 链霉素亲和素微珠的特点:•组成:硅烷化氧化铁•磁化:25-35 EMU/g•磁化类型:超顺磁性了解更多信息
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货号数量
213445 mL
货号 21344
价格(CNY)
8,772.00
Each
添加至购物车
数量:
5 mL
请求批量或定制报价
价格(CNY)
8,772.00
Each
添加至购物车
Thermo Scientific MagnaBind 链霉素亲和素微珠方便用于涉及生物素标记分子的亲和纯化或分离方法。为从悬浮液中取出 MagnaBind 微珠,请使用外部磁场。

MagnaBind 链霉素亲和素微珠的特点:

组成:硅烷化氧化铁
磁化:25-35 EMU/g
磁化类型:超顺磁性(无磁性记忆)
表面积:> 100 m2/g
微珠大小:1-4 μm 直径
沉降速率:30 分钟内 4%
有效密度:2.5 g/mL
微珠数量:1 × 108 微珠/mg
pH 值稳定性:水溶液,pH 值高于 4.0
浓度:5 mg/mL

链霉素亲和素是一种来自 Streptomycetes avidinii 的 60 kDa 蛋白。该蛋白质是一种四聚物,含有四个生物素结合位点。与亲和素不同,链霉素亲和素的等电点较低 (pI=5),且无碳水化合物基团,从而产生较低非特异性结合。除非条件非常恶劣,如在 SDS-PAGE 的上样缓冲液或 8 M 盐酸胍(pH 值 1.5)中煮沸,否则链霉素亲和素与生物素之间的高亲和相互作用无法有效分离。因此,通常可能洗脱相互作用复合物中的结合配偶体,而不会洗脱生物素化组分。

应用:
•使用阳性或阴性选择进行的细胞分选
•使用直接或间接方法进行的蛋白纯化或免疫检测
仅供科研使用。不可用于诊断程序。
规格
最大浓度Slurry: 5 mg/mL
描述MagnaBind 链霉素微珠
配基类型链霉亲和素
产品线MagnaBind™
数量5 mL
容积(公制)5 mL
颜色棕色
直径(公制)1 μm 至 4 μm
适用于(应用)结合约 2ug(8nmol)生物素/mL 微珠
产品类型链霉亲和素微珠
Unit SizeEach
内容与储存
接收后,请将产品储存在 4°C 下。切勿冷冻产品。产品在环境温度下运输。

常见问题解答 (FAQ)

What are the differences between Pierce Streptavidin Magnetic Beads and MagnaBind Streptavidin Beads?

Pierce Streptavidin Magnetic Beads are homogeneous, non-aggregating, pre-blocked, iron oxide, superparamagnetic microparticles encapsulated with a proprietary polymer surface (no exposed iron, bead size of approximately 1 (0.5-1.5) µm, concentration 10 mg/mL). Pierce Streptavidin Magnetic Beads can be used in manual or automated applications (using KingFisher 96 and KingFisher Flex instruments, for example).
MagnaBind Streptavidin Beads are also superparamagnetic particles, but are heterogeneous aggregates made of silanized iron oxide (bead size approximately 1-4 µm, concentration 5 mg/mL). MagnaBind Streptavidin Beads can only be used in manual applications.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I centrifuge the MagnaBind Streptavidin Beads and Thermo Scientific Streptavidin Magnetic Beads?

No, centrifuging causes the formation of irreversible aggregates which greatly reduces binding capacity.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I freeze the MagnaBind Streptavidin Beads and Thermo Scientific Streptavidin Magnetic Beads?

No, freezing or drying will cause the beads to aggregate and lose binding activity. Store the beads only at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What are the average sizes of your streptavidin magnetic beads?

Thermo Scientific Streptavidin Magnetic Beads average about 1 micron in daimeter ranging from about 0.5-1.5 microns while MagnaBind Streptavidin Beads are heterogenous averaging 1 - 4 microns.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are the MagnaBind Streptavidin Beads and Thermo Scientific Streptavidin Magnetic Beads sterile?

No, neither the MagnaBind Streptavidin Beads nor the Thermo Scientific Streptavidin Magnetic Beads are sterilized. However, they are both supplied in solutions containing sodium azide to prevent bacterial growth.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.