MagnaBind™ 蛋白 A 微珠
MagnaBind™ 蛋白 A 微珠
Thermo Scientific™

MagnaBind™ 蛋白 A 微珠

能够使用这些配体包被的(链霉素亲和素、蛋白 A 和 G、GAM IgG、GAR IgG)磁粒子和磁分离器进行实验台磁亲和纯化。
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货号数量
213485 mL
货号 21348
价格(CNY)
12,617.00
Each
添加至购物车
数量:
5 mL
请求批量或定制报价
价格(CNY)
12,617.00
Each
添加至购物车
Thermo Scientific™ MagnaBind 磁性微球可与链霉素、蛋白A、蛋白G、GAM IgG、GAR IgG和其他配基一起用于各种小规模的蛋白质的磁性亲和分离。
MagnaBind 磁性微球无需使用分离柱和离心步骤,即可进行小规模亲和纯化和细胞分离。微球即硅烷化氧化铁磁性微粒,使用不同的亲和配基进行了活化。在亲和纯化程序中,完成了结合步骤后,将微量离心管或反应管置于适当的稀土磁铁附近,即可简便而快速地收集到磁性微粒。MagnaBind 微球可快速响应 MagnaBind 磁场。由于不带有任何磁性记忆,这些微粒可以被轻松地反复分散和聚集(即颗粒的聚集是可逆的)。

现可提供的MagnaBind 微球包括:预先包被有蛋白A、蛋白G、链亲和素、抗小鼠或抗兔抗体的MagnaBind 微球。还可提供定制服务,将微球使用氨基或羧基活化,这些基团作为个性化共价固定蛋白或配基的靶标。

特点:

  • 可预包被广泛采用的亲和配体或衍生化用于蛋白和其他特异性配体的共价结合
  • 由于微珠无磁性记忆,不会发生不可逆的聚集;如果需要,多次收集和分散微珠
  • 大多数分离需要较短的 5-10 分钟试验台程序

未衍生化 MagnaBind 微珠的特性:

  • 成分:硅烷化氧化铁
  • 磁化:25 至 35 EMU/g
  • 磁化类型:超顺磁性(无磁性记忆)
  • 表面积:大于 100 m2/g
  • 微珠大小:直径 1 至 4 μm
  • 沉降速率:30 分钟内 4%
  • 有效密度:2.5g/mL
  • 微珠数量:每毫升 1 x 108 微珠
  • pH 值稳定性:水溶液,pH 值高于 4.0
  • 浓度:5mg/mL

需要:

使用外部磁场从悬浮液中去除MagnaBind微球;对于磁场的选择,请参阅订货表。

建议用于:

使用阳性或阴性选择进行细胞分选;使用直接或间接方法进行蛋白纯化或免疫分析;免疫沉淀反应

仅供科研使用。不可用于诊断程序。
规格
最大浓度Slurry: 5 mg/mL
描述MagnaBind蛋白A颗粒
配基类型蛋白 A
数量5 mL
足够用于0.2 mg 兔 IgG/mL 微珠
靶标抗体
容量(公制)5 mL
形式液体
粒径1 to 4 μm
产品线MagnaBind™
类型磁珠
Unit SizeEach
内容与储存
接收后,在 4°C 下储存产品。切勿冷冻产品。这些产品在环境温度下运输。

常见问题解答 (FAQ)

What are the differences between Protein A, Protein G, Protein A/G, and Protein L Ig-binding proteins?

Protein A, Protein G, and Protein A/G bind almost exclusively to the IgG class of antibodies, but their binding properties differ among species and subclasses of IgG. Protein L binds in the variable fragment of some kappa light chains and can react with any immunoglobulin, not just IgG, as long as the correct kappa light chains are present. Protein L does not bind lambda light chains and certain kappa chains of different species.

-Protein A is generally preferred for rabbit, pig, dog, and cat IgG.
-Protein G has better binding capacity for a broader range of mouse and human IgG subclasses (e.g., IgG1 vs. IgG2)
-Protein A/G is a recombinant fusion protein that includes the IgG-binding domains of both Protein A and Protein G. Therefore, Protein A/G is ideal for binding the broadest range of IgG subclasses from rabbit, mouse, human, and other mammalian samples.
-Protein L binds to certain immunoglobulin kappa light chains. Because kappa light chains occur in members of all classes of immunoglobulin (i.e., IgG, IgM, IgA, IgE and IgD), Protein L can purify these different classes of antibody. However, only those antibodies within each class that possess the appropriate kappa light chains will bind. Generally, empirical testing is required to determine if Protein L is effective for purifying a particular antibody. It binds only Vk1 in mouse and VkI, VkIII and VkIV in human.
Read more about the general characteristics of Ig-binding proteins (https://www.thermofisher.com/us/en/home/life-science/antibodies/antibody-purification-kits-reagents.html) and (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0034-Ab-binding-proteins.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.