SPB（琥珀酰亚胺基-[4-(补骨脂素-8-氧基)]-丁酸酯）
SPB（琥珀酰亚胺基-[4-(补骨脂素-8-氧基)]-丁酸酯）
Thermo Scientific™

SPB（琥珀酰亚胺基-[4-(补骨脂素-8-氧基)]-丁酸酯）

Thermo Scientific Pierce SPB is an NHS-ester and psoralen crosslinker that effectively conjugates primary amines to DNA via light-activated intercalation了解更多信息
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货号数量
2301350 mg
货号 23013
价格（CNY)
4,965.00
Each
添加至购物车
数量:
50 mg
价格（CNY)
4,965.00
Each
添加至购物车
Thermo Scientific Pierce SPB is an NHS-ester and psoralen crosslinker that effectively conjugates primary amines to DNA via light-activated intercalation next to pyrimidine bases.

Features of SPB:

• Reactive groups: NHS ester and psoralen
• Reactive toward: amino groups and thymine
• DNA intercalating agent and nucleic acid probe1,2
• Reagent for photo-immobilization to microplates3
• Melting point: 177-178°C

Product References:
Crosslinker Application Guide -- search for recent literature references for this product
仅供科研使用。不可用于诊断程序。
规格
细胞渗透性Not determined
描述SPB
形式实心
标记方法化学标记
分子量385.32
聚乙二醇化否
产品线Pierce
数量50 mg
反应一部分补骨脂素、NHS 酯
运输条件环境
溶解度DMF、DMSO
间隔臂长8.6 Å
水溶性否
化学反应性胺-非选择性
可裂解否
交联剂类型异型双功能团试剂
产品规格标准
产品类型交联剂
间隔子短 (<10 Å)
Unit SizeEach
内容与储存
接收后，请在 4°C 下干燥储存。

常见问题解答 (FAQ)

Can you provide the shelf-life for SPB (succinimidyl-[4-(psoralen-8-yloxy)]-butyrate)?

SPB (succinimidyl-[4-(psoralen-8-yloxy)]-butyrate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What are the options for crosslinking proteins to nucleic acids, and what are the differences between them?

We offer the following crosslinkers for protein to nucleic acids: formaldehyde (Cat. No. 28906), SPB (Cat. No. 23013), SDAD (Cat. No. 26169), sulfo-SDAD (Cat. No. 26175), and sulfo-SBED (Cat. No. A39260).
Formaldehyde can be used for DNA-DNA crosslinking as well as DNA-protein crosslinking. While it is good for crosslinking proteins that are in direct contact with DNA, formaldehyde cannot link proteins that may be bound in a complex with other proteins but are not in direct contact with DNA. EGS or DSG can crosslink proteins to other proteins that are in direct contact with DNA and crosslinked to DNA by formaldehyde.
SPB, Sulfo-SDAD, and Sulfo-SBED are all photoreactive crosslinkers. SPB is an NHS-ester and psoralen heterobifunctional crosslinker that conjugates primary amines on proteins to DNA via photo-activated intercalation of psoralen to pyrimidine bases. The psoralen tricyclic planar ring system intercalates into double-stranded, and to a lesser extent, single-stranded DNA and RNA. The photoreactive psoralen group provides more selective covalent binding to nucleic acids than either phenyl azide or diazerine linkers, which makes SPB the best choice. The diazerine on sulfo-SDAD and the phenyl azide on sulfo-SBED are not selective for nucleic acids and can crosslink other biomolecules in the same besides any protein-DNA interactions. Sulfo-SBED involves biotin transfer rather than actual linking.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.