L-谷氨酰胺 (200 mM)
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L-谷氨酰胺 (200 mM)
引用和文献 (10)
Gibco™

L-谷氨酰胺 (200 mM)

L-谷氨酰胺是细胞培养所必需的一种氨基酸。L-谷氨酰胺参与嘌呤和嘧啶核苷酸、氨基糖类、谷胱甘肽、L-谷氨酸和其他氨基酸的形成，并参与蛋白质的合成和葡萄糖的产生。与大多数其他氨基酸不同，L-谷氨酰胺在溶液中不稳定。降解速率是时间、温度和 pH 的函数了解更多信息
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货号数量
25030081100 mL
2503014920 mL
2503016420 x 100 mL
货号 25030081
价格（CNY)
497.00
飞享价
Ends: 27-Dec-2025
688.00
共减 191.00 (28%)
Each
添加至购物车
数量:
100 mL
Customize this product
价格（CNY)
497.00
飞享价
Ends: 27-Dec-2025
688.00
共减 191.00 (28%)
Each
添加至购物车
L-谷氨酰胺是细胞培养所必需的一种氨基酸。L-谷氨酰胺参与嘌呤和嘧啶核苷酸、氨基糖类、谷胱甘肽、L-谷氨酸和其他氨基酸的形成，并参与蛋白质的合成和葡萄糖的产生。与大多数其他氨基酸不同，L-谷氨酰胺在溶液中不稳定。降解速率是时间、温度和 pH 的函数。Gibco™ L-谷氨酰胺是一种即用型 200 mM 储备液，可作为细胞培养补充剂。较佳浓度取决于用于培养细胞的细胞类型和培养基，但通常在 2–6 mM 范围内。

我们还提供 Gibco™ GlutaMAX™ 补充剂作为 L-谷氨酰胺的稳定替代产品。

双生产现场 cGMP 生产和质量体系
Gibco™ L-谷氨酰胺是在位于英国苏格兰佩斯利的一家符合 cGMP 要求的工厂内生产的。该工厂是在 FDA 注册的医疗器械生产商，且通过 ISO 13485 标准认证。为确保供应链连续性，我们同时提供由我们格兰德岛工厂生产的等同 Gibco™ L-谷氨酰胺产品 (25030-081)。本工厂者亦是在 FDA 登记的医疗器械生产商，且符合 ISO 13485 标准。
规格
化学名称或材料添加剂
浓度或成分（按分析物或组分）100 X
环保功能绿色可持续包装
物理形态液体
产品线Gibco
建议的储存条件储存条件：-5°C 至 -20°C。避光。
运输条件：干冰
有效期：自生产之日起 24 个月
有效期24个月
运输条件干冰
数量100 mL
pH6 - 8
Unit SizeEach

常见问题解答 (FAQ)

为何我的200 mM L-谷氨酰胺在化冻后出现沉淀？

浓储存液中的L-谷氨酰胺在低温下易于发生沉淀。在37°C水浴中短暂加热储存液并辅以温和的摇动，就可溶解沉淀。在沉淀完全溶解之前不要使用本品。

Why did my 200 mM L-Glutamine precipitate out of solution when I thawed it?

When L-glutamine is in a concentrated stock solution it easily precipitates when cooled. Warming the solution briefly in a 37C water bath with gentle swirling will dissolve the precipitate. Do not use the product unless the precipitate is fully dissolved.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Why does L-Glutamine (200 mM) (Cat. No. 25050149, 25030081, 25030164) solution have “Product of Brazil” on the label?

L-Glutamine (200 mM) (Cat. No. 25050149, 25030081, 25030164) solution is made in the USA from a raw material glutamine powder manufactured in Brazil. The country of manufacture of a component that has been subjected to manufacturing or processing operations in more than one country is the last country in which the component has been “substantially transformed”. A “substantial transformation” occurs in a country when a product emerges from a manufacturing process with a name, character, or use different from that of the original material(s) subjected to that process.

Diluting, purifying, sorting, testing, packing, repacking, or affixing labels to a product in a country does not constitute a “substantial transformation” in that country, hence the label must bear the country of the original raw material origin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My order of L-Glutamine (Cat. No. 25030081) arrived frozen. Is it stable?

If L-Glutamine is completely or partially frozen, it is still stable. It becomes unstable when stored for extended periods of time completely thawed, especially above 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is your recommended method for thawing L-Glutamine before adding to cell culture media?

We recommend thawing at 2-8 degrees C and then warming at 37 degrees C until the material goes into solution. Mix it as it warms up to spend minimal time at elevated temperatures before you aliquot it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all L-谷氨酰胺 (200 mM) FAQs

引用和文献 (10)

引用和文献
Abstract
Structural and energetic characteristics of the heparin-binding site in antithrombotic protein C.
Authors:Friedrich U, Blom AM, Dahlbäck B, Villoutreix BO,
Journal:J Biol Chem
PubMed ID:11316800
'Human activated protein C (APC) is a key component of a natural anticoagulant system that regulates blood coagulation. In vivo, the catalytic activity of APC is regulated by two serpins, alpha1-antitrypsin and the protein C inhibitor (PCI), the inhibition by the latter being stimulated by heparin. We have identified a ... More
FRET measurements of intracellular cAMP concentrations and cAMP analog permeability in intact cells.
Authors:Börner S, Schwede F, Schlipp A, Berisha F, Calebiro D, Lohse MJ, Nikolaev VO,
Journal:Nat Protoc
PubMed ID:21412271
'Real-time measurements of second messengers in living cells, such as cAMP, are usually performed by ratiometric fluorescence resonance energy transfer (FRET) imaging. However, correct calibration of FRET ratios, accurate calculations of absolute cAMP levels and actual permeabilities of different cAMP analogs have been challenging. Here we present a protocol that ... More
A critical role for choline kinase-alpha in the aggressiveness of bladder carcinomas.
Authors:Hernando E, Sarmentero-Estrada J, Koppie T, Belda-Iniesta C, Ramírez de Molina V, Cejas P, Ozu C, Le C, Sánchez JJ, González-Barón M, Koutcher J, Cordón-Cardó C, Bochner BH, Lacal JC, Ramírez de Molina A,
Journal:Oncogene
PubMed ID:19448670
'Bladder cancer is one of the most common causes of death in industrialized countries. New tumor markers and therapeutic approaches are still needed to improve the management of bladder cancer patients. Choline kinase-alpha (ChoKalpha) is a metabolic enzyme that has a role in cell proliferation and transformation. Inhibitors of ChoKalpha ... More
Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O.
Authors:Walev I, Bhakdi SC, Hofmann F, Djonder N, Valeva A, Aktories K, Bhakdi S,
Journal:Proc Natl Acad Sci U S A
PubMed ID:11248053
'The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10(5)-10(6) molecules were estimated to be entrapped per cell. Repair of toxin lesions depended on Ca(2+)-calmodulin and on ... More
Fabrication of synthetic polymer coatings and their use in feeder-free culture of human embryonic stem cells.
Authors:Nandivada H, Villa-Diaz LG, O'Shea KS, Smith GD, Krebsbach PH, Lahann J,
Journal:Nat Protoc
PubMed ID:21720316
The culture of human embryonic stem (hES) cells in defined and xenogeneic-free conditions will contribute substantially to future biotechnological and medical applications. To achieve this goal, we developed the first fully defined synthetic polymer coating poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH) that sustains long-term growth of hES cells in different culture media. ... More
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