胎牛血清，透析，新西兰来源

Gibco™ 胎牛血清在基础细胞培养、专业研究和特定检测方面具有较高的应用价值，深受研究人员信赖。其屡获殊荣，可以提供稳定的质量，满足研究需求和预算要求。此透析胎牛血清分类：•用 0.1μm 的过滤器进行三重过滤，透析后• 内毒素水平：≤10 EU/mL• 血红蛋白水平：≤25了解更多信息

货号	数量
30067334	500 mL

货号 30067334

价格（CNY)

22,233.00

添加至购物车

500 mL

价格（CNY)

22,233.00

添加至购物车

Gibco™ 胎牛血清在基础细胞培养、专业研究和特定检测方面具有较高的应用价值，深受研究人员信赖。其屡获殊荣，可以提供稳定的质量，满足研究需求和预算要求。

此透析胎牛血清分类：
•用 0.1μm 的过滤器进行三重过滤，透析后
• 内毒素水平：≤10 EU/mL
• 血红蛋白水平：≤25 mg/dL
• 来源：新西兰
• 标上仅供体外诊断使用

在一些国家将此类试剂归为医疗器械。如果其在特定地区未被列为医疗器械进行监管，则应仅供实验室使用。

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.

处理用 0.1 μm 的过滤器进行三重过滤

运输条件冰冻

原产国新西兰

产品类型胎牛血清

数量500 mL

血清处理已透析

灭菌方法使用 0.1 μm 过滤器进行三重过滤

Unit SizeEach

内容与储存

储存条件：≤-10°C
运输条件：冰冻

常见问题解答 (FAQ)

如果收到的FBS已经部分化冻，我还能继续使用么？

我们的研究结果显示在4°C条件下短时保存化冻的FBS，28天（之内）不会降低其生长或活性方面的效能。如需在4°C条件下将FBS保存更长时间，就需要逐案评估这一储存条件是否为必须。

我有时在血清中见到絮状沉淀。那可能是些什么物质？

多种原因可能导致FBS中出现絮状沉淀。最为常见的原因是血清中脂蛋白的变性。你可能会在血清化冻后观察到血纤蛋白——一种血清中存在的促凝蛋白。这并不会影响本品的使用性能。

如需去除絮状沉淀，请将血清移至无菌管中，以400 x g进行简短离心。之后把所得的上清液和培养基一并过滤。不要试图过滤含有絮状沉淀的血清，可能会堵塞滤器。

存放于冰箱中的FBS中为何会出现沉淀？

Gibco FBS未经预老化处理。当储存于2至8°C的条件下，血清中可能存在的各类蛋白与脂蛋白（如冷凝集素，纤维蛋白原，玻连蛋白等）会发生聚集，并且形成肉眼可见的沉淀或混浊。这并不会影响血清的使用性能。我们推荐您将FBS储存于–20°C，同时避免反复冻融。

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol¹.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

If my FBS arrives partially thawed, can I still use it?

Our studies have shown that short-term storage of thawed FBS at 4 degrees C for up to 28 days causes no decrease in growth or viability performance. FBS stored at 4 degrees C for longer periods of time should be evaluated on a case-by-case basis should this storage condition be necessary.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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