TaqMan™ β-Actin 检测试剂
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Applied Biosystems™

TaqMan™ β-Actin 检测试剂

Applied Biosystems™ TaqMan™ β-actin 提供必要组分，以便使用 β-actin 引物作为采用人核酸的 TaqMan™ 实时荧光定量 PCR了解更多信息
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货号数量
401846100 reactions
货号 401846
价格（CNY)
4,350.00
Each
添加至购物车
数量:
100 reactions
价格（CNY)
4,350.00
Each
添加至购物车
Applied Biosystems™ TaqMan™ β-actin 提供必要组分，以便使用 β-actin 引物作为采用人核酸的 TaqMan™ 实时荧光定量 PCR 应用中的归一化对照。可以使用 DNA 或 RNA 作为目标模板。

•预设计引物和 FAM 标记 TaqMan™ 探针可避免测定试剂设计

•快速测定开发指南可以尽量缩短优化时间

•各组分为测定试剂配制提供灵活性

使用注意事项

TaqMan™ β-肌动蛋白检测试剂盒可与 TaqMan™ 一步法 RT-PCR 预混液试剂、TaqMan™ EZ RT-PCR 核心试剂或 TaqMan™ Gold RT-PCR 试剂配合使用，以进行一步法逆转录聚合酶链反应 (RT-PCR)。

购买者须知
1.许可免责声明：上述产品可用于 Roche Molecular Systems, Inc.以及 F. Hoffmann-La Roche Ltd. 所拥有的的专利保护的聚合酶链反应 (“PCR”) 方法。但是，购买本品并未明示或暗示向购买者转让使用 PCR 流程的专利许可。

TaqMan™ 探针受 PE Corporation (NY) 所拥有的美国专利5,723,591及他国相应专利保护，并受 Applied Biosystems™ 认可的美国专利5,801,155和6,084,102及他国相应专利的保护

注意：有关有限的标签许可和商标信息，请参见用户手册或包装说明书。仅供科研使用。不适用于诊断用途。
仅供科研使用。不可用于诊断程序。
规格
控制模板人类对照 DNA
产品规格管装
基因符号ACTB
内部探针修改TAMRA™ Quencher (3')，FAM (5')
反应次数100 次反应
产品线TaqMan
产品类型实时荧光定量 PCR 试剂
数量100 reactions
运输条件干冰
种属人
足够用于100 次反应
靶标β-肌动蛋白
标签或染料FAM
PCR 方法一步法 RT-qPCR
Unit SizeEach
内容与储存
对照模板

常见问题解答 (FAQ)

Can I use the TaqMan β-actin Detection Reagents (Cat. No. 401846) as an endogenous control for my gene expression studies?

The TaqMan β-actin Detection Reagents were designed to amplify genomic DNA, and thus are not recommended for use as an endogenous control for gene expression studies. However, we do offer other TaqMan assay reagents that were specifically designed for that application. Two examples are: Human ACTB (Beta Actin) Endogenous Control, Cat. No. 4310881E (TAMRA probe) or Cat. No. 4326315E (MGB probe).

There are many factors to consider when validating a specific endogenous control. For additional information on designing gene expression assays and controls, please refer to the following documents: "Protocol: TaqMan Human Endogenous Control Plate", and User Bulletin #2: "Relative Quantitation of Gene Expression". You can find copies of these documents on our website by entering the titles in the main Search field.

Can TaqMan Beta-actin Detection Reagents, Cat. No. 401846, be used for both human and mouse genomic DNA?

This particular kit was designed for human DNA. Our current Beta-actin Detection Reagents are designed for human targets only. Please check our online search tools for other Beta actin reagents for human (Hs), mouse (Mm) or rat (Rn) targets.

Where can I find the TaqMan Actin Detection Reagents (P/N 401846) manual?

Complete instructions for the TaqMan B-actin Detection Reagents can be found in the manual titled "TaqMan PCR Reagent Kit with Controls" on the product page.

引用和文献 (1)

引用和文献
Abstract
In situ detection of starvation-induced autophagy.
Authors:Martinet W, De Meyer GR, Andries L, Herman AG, Kockx MM,
Journal:J Histochem Cytochem
PubMed ID:16148314
Autophagy is a regulated bulk degradation process involved in many different human pathologies. Transmission electron microscopy (TEM) is currently the only reliable method for monitoring autophagy in situ. Because TEM is labor intensive, we questioned whether useful marker proteins can be found for unambiguous detection of autophagy in tissue via ... More