TaqMan™ 反转录试剂
TaqMan™ 反转录试剂
引用和文献 (2)
Invitrogen™

TaqMan™ 反转录试剂

TaqMan™ 反转录试剂提供用于反转录酶 PCR 的必要试剂。包括随机六聚体、寡核苷酸 (dT)16 以及反向引物以保证 cDNA 合成灵活性了解更多信息
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货号反应次数
43041342,000 次反应
N8080234200 次反应
货号 4304134
价格（CNY)
74,797.00
Each
添加至购物车
反应次数:
2,000 次反应
价格（CNY)
74,797.00
Each
添加至购物车
TaqMan™ 反转录试剂提供用于反转录酶 PCR 的必要试剂。包括随机六聚体、寡核苷酸 (dT)16 以及反向引物以保证 cDNA 合成灵活性。TaqMan™ 反转录试剂的优点：

•包含 MultiScribe™ 反转录酶，一种重组莫洛尼小鼠白血病病毒反转录酶 (MMLV RT)
• 包括随机六聚体和寡核苷酸 (dT)16 以保证灵活性
• 单个组分为测定试剂配制提供多功能性
• 与两步法 RT-PCR 反应相容

使用 TaqMan™ 反转录试剂
TaqMan™ 反转录试剂提供将 RNA 反转录成 cDNA 的所有必要组分。对于两步法 RT-PCR，随机六聚体、寡核苷酸 (dT)16 和序列特异性反向引物全都可以使用。最好在用实验方法评估所有三种引物系统后选择反转录引物。
仅供科研使用。不可用于诊断程序。
规格
最终产品类型第一链 cDNA
产品规格试剂盒
反应次数2,000 次反应
最佳反应温度42°C
数量2000 次反应
反应形式单独组分
试剂类型反转录
逆转录酶MultiScribe™
核糖核酸酶 H 活性是
运输条件干冰
尺寸（最终产品）长达 5 kb
原始材料RNA
技术反转录
最大浓度50 U/μL
适用于（应用）实时 PCR (qPCR)
Unit SizeEach
内容与储存

包括10个单位(货号:N8080234),每个单位包含:

• MultiScribe 逆转录酶,100 μL (50 U/μL)
• RNase 抑制剂,100 μL (20 U/μL)
• dNTP 混合物,1 mL(每种 dNTP 2.5 mM)
• Oligo(dT)16100 μL (50 μM)
• 随机六聚体,100 μL (50 μM)
• 10X RT 缓冲液,1.5 mL
• MgCl2 溶液,1.5 mL

将试剂储存在 –25°C 至 –15°C 下。

常见问题解答 (FAQ)

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

Can the RT buffer for TaqMan Reverse Transcription Reagents kit (Cat. No. N8080234) be purchased separately?

Yes, it is the same as the GeneAmp 10X PCR Buffer II & MgCl2 (Cat. No. N8080010).

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

引用和文献 (2)

引用和文献
Abstract
Combined analysis of levels of serum B-cell activating factor and a proliferation-inducing ligand as predictor of disease progression in patients with chronic lymphocytic leukemia.
Authors:Ferrer G, Hodgson K, Pereira A, Juan M, Elena M, Colomer D, Roué G, Aymerich M, Baumann T, Montserrat E, Moreno C
Journal:Leuk Lymphoma
PubMed ID:21707303
'B-cell activating factor (BAFF) and a proliferation-inducing ligand (APRIL) are regulators of normal B-cell development and survival. We investigated their role in chronic lymphocyticleukemia (CLL) by relating serum protein levels and CLL cell mRNA expression with clinical factors and disease progression. In patients with CLL, BAFF serum levels were significantly ... More
Regulating type 1 IFN effects in CD8 T cells during viral infections: changing STAT4 and STAT1 expression for function.
Authors:Gil MP, Ploquin MJ, Watford WT, Lee SH, Kim K, Wang X, Kanno Y, O'Shea JJ, Biron CA
Journal:Blood
PubMed ID:22968462
Type 1 IFNs can conditionally activate all of the signal transducers and activators of transcription molecules (STATs), including STAT4. The best-characterized signaling pathways use STAT1, however, and type 1 IFN inhibition of cell proliferation is STAT1 dependent. We report that type 1 IFNs can basally stimulate STAT1- and STAT4-dependent effects ... More