MultiScribe™ 反转录酶

引用和文献 (1)

Invitrogen™

MultiScribe™ 反转录酶

MultiScribe™ 反转录酶是一种重组型莫洛尼鼠白血病病毒 (rMoMuLV) 反转录酶，经优化可用于 TaqMan 分析。MultiScribe™ 反转录酶与 MuLV 反转录酶相似，但推荐用途不同了解更多信息

货号	数量
4311235	100 μL

货号 4311235

价格（CNY)

1,652.00

添加至购物车

100 μL

价格（CNY)

1,652.00

添加至购物车

MultiScribe™ 反转录酶是一种重组型莫洛尼鼠白血病病毒 (rMoMuLV) 反转录酶，经优化可用于 TaqMan 分析。MultiScribe™ 反转录酶与 MuLV 反转录酶相似，但推荐用途不同。MultiScribe™ 反转录酶是一种重组型 RNA–依赖性 DNA 聚合酶，可在存在引物的情况下使用单链 RNA 为模板合成互补 DNA (cDNA) 链。以每瓶5,000 U 的100μL 瓶装形式提供 (50U/μL)。MultiScribe™ 反转录酶的优势：

•兼容一步法和两步法 RT-PCR 反应，为反应构建带来了灵活选择
•单独的组分设计为反应构建带来了多样选择

仅供科研使用。不可用于诊断程序。

最终产品类型第一链 cDNA

产品规格管装

最佳反应温度37°C

数量100 μL

反应形式单独组分

试剂类型反转录

逆转录酶MultiScribe™

核糖核酸酶 H 活性减少

运输条件干冰

尺寸（最终产品）长达 7 kb

原始材料RNA

技术反转录

最大浓度50 U/μL

适用于（应用）RT-PCR

Unit SizeEach

内容与储存

• MultiScribe™ 逆转录酶，100 μL (50 U/μL)

储存在 –20°C 下。

常见问题解答 (FAQ)

PCR反应要加入多少第一链cDNA反应产物？

尽管实际体积取决于用于第一链合成反应的RNA起始量和靶标基因的丰度，但我们建议使用10%的第一链cDNA反应产物进行PCR反应。

How much of the first-strand cDNA reaction should I load for PCR?

While the volume is dependent on the starting amount of RNA used for the first-strand synthesis and the abundance of the target gene, we'd recommend starting with 10% of the first-strand reaction for your PCR reaction.

Can I purchase the MultiScribe Reverse Transcriptase from the TaqMan® MicroRNA Reverse Transcription Kit (Cat. No 4366596) separately?

Yes, the MultiScribe Reverse Transcriptase (Cat. No. 4311235) can be purchased as a standalone product. Please see the following link.

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Does Applied Biosystems MultiScribe Reverse Transcriptase have RNase H activity?

Yes, MultiScribe Reverse Transcriptase (Cat. No. 4311235) does have RNase H activity.

What is the difference between MultiScribe Reverse Transcriptase and MuLV Reverse Transcriptase?

MultiScribe Reverse Transcriptase (Cat. No. 4311235) and MuLV Reverse Transcriptase (Cat. No. N808-0018) are the same enzyme, a recombinant Moloney Murine Leukemia Virus (MuLV) Reverse Transcriptase. The only difference is in the recommended usage for each enzyme. MultiScribe Reverse Transcriptase is recommended for use with quantitative nuclease assays, and MuLV is recommended for use in traditional RT-PCR assays.

引用和文献 (1)

引用和文献

Abstract

PAMP induced expression of immune relevant genes in head kidney leukocytes of rainbow trout (Oncorhynchus mykiss).

Authors:Chettri JK, Raida MK, Holten-Andersen L, Kania PW, Buchmann K

Journal:Dev Comp Immunol

PubMed ID:21147161

'Host immune responses elicited by invading pathogens depend on recognition of the pathogen by specific receptors present on phagocytic cells. However, the reactions to viral, bacterial, parasitic and fungal pathogens vary according to the pathogen-associated molecular patterns (PAMPs) on the surface of the invader. Phagocytic cells are known to initiate ... More