How can I convert a file from AQ to RQ, or from RQ to AQ file with the 7900HT Fast Real-Time PCR System?
SDS v2.4 and above allow you to convert your AQ files to RQ and vice versa.
To do so, simply go to: Tools then AQ RQ Converter
-Choose from the dropdown which direction you want to go (AQ to RQ, or RQ to AQ).
-Next click on ‘Add' and browse to the plate file location in the new window. and click ‘Open'.
-Click on ‘Convert'. A new file will be created in the same location as the original, but with _AQ or _RQ at the end of the filename.
If you do not have v2.4, you can upgrade your software here (http://www.thermofisher.com/us/en/home/technical-resources/software-downloads/applied-biosystems-7900ht-fast-real-timespcr-system.html).
One benefit of this conversion feature is that it allows you to convert 7900 files to the correct type to do analysis for relative quantification study, for example, if a plate is originally run as AQ instead of RQ, or if you would like to generate a standard curve quantification in the AQ if a plate was originally run as RQ.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Why are there no RQ values for all the samples in my study in the 7900HT Fast Real-Time PCR System software?
The 7900HT Fast Real-Time PCR System software requires that the endogenous control be run on every plate within a study. If not, you will only see RQ values for the plate that contains the endogenous control on it. The other plates will have Ct values for all the wells, but the software will not calculate RQ values. If you designed your experiments in this type of layout, you will want to do your analysis in the free DataAssist Software (https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/dataassist-software.html?icid=fr-realtimesoftware-5) instead, or use the Thermo Fisher Cloud (https://www.thermofisher.com/us/en/home/cloud.html). This program does not have the endogenous control requirement for every plate. Simply export the study results file from the 7900HT software as a *.txt or *.csv file, and use that to create a new study in the DataAssist software. You should now be able to see RQ values for all samples, even those without an endogenous control on the same plate.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
Why do I not see any Ct or RQ values from my ddCT experiment in the 7900HT Fast Real-Time PCR System software?
In the 7900HT Fast Real-Time PCR System software, v2.3 or later, CT or RQ values are only provided in the RQ Manager software. RQ Manager is installed on the computer along with SDS v2.x.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
What can I use for data analysis on the 7900HT Fast Real-Time PCR System?
For genotyping data, we recommend TaqMan Genotyper Software (https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/taqman-genotyper-software.html?icid=fr-software-1).
For relative quantitation, we recommend ExpressionSuite Software (https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/expressionsuite-software.html). You may also use the Thermo Fisher Cloud for either application. https://www.thermofisher.com/us/en/home/cloud.html
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
What dyes can I use on the 7900HT Fast Real-Time PCR System?
The Applied Biosystems 7900HT Fast Real-Time PCR Systems use the following dye sets for calibration: FAM, SYBR, VIC, ROX, NED, TAMRA, JOE, TET dyes. Custom dyes can also be used that read between 500 nm and 660 nm, although you will have to calibrate the system first for any new dye.
Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.
