带有 384 孔区块模块和自动化配件的 7900HT Fast 实时荧光定量 PCR 系统
带有 384 孔区块模块和自动化配件的 7900HT Fast 实时荧光定量 PCR 系统
Applied Biosystems™

带有 384 孔区块模块和自动化配件的 7900HT Fast 实时荧光定量 PCR 系统

7900HT Fast 实时荧光定量 PCR 系统是一种实时定量 PCR 系统,它将 96 和 384了解更多信息
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货号数量
43290021 instrument
货号 4329002
价格(CNY)
-
申请报价
数量:
1 instrument
7900HT Fast 实时荧光定量 PCR 系统是一种实时定量 PCR 系统,它将 96 和 384 孔板的兼容性和 TaqMan™ 低密度阵列与全自动机械臂上样功能相结合,现在还提供选配的 Fast 实时荧光定量 PCR 功能。选配的集成自动化配件可提供较大通量。一次可加载多达 84 个 384 孔板,用于无人照看操作;条形码读码器允许您随时将平板插入或替换到样品队列中。

•Fast PCR 选项可将标准 96 孔规格中的运行时间缩短至约 35 分钟,或将 384 孔板中的运行时间缩短至约 55 分钟。
• 500-660 nm 的连续波长检测允许在单次反应中使用多个荧光基团
• 测量基因表达水平,检测和定量测定病原体,进行等位基因分型(SNP 基因分型)分析,以及对基因序列的存在进行评分
• 96 或 384 孔板兼容性(包括 TaqMan™ 低密度阵列)
• 选配的企业版软件提供支持 21 CFR 第 11 部分指南中数据完整性和安全性相关规定的数据分析工具
• 免手动操作板加载和卸载可提供真正无人值守的自动化,使您可以提高实验室的工作效率
• 经证实的检测开发指南可节省时间和资金

高通量
7900HT 系统是一种检测和定量测定核酸序列的高通量实时荧光定量 PCR 系统。选配的自动化配件与 384 孔板功能相结合,使 7900HT 系统非常适合满足当今药物发明过程的高通量要求。主要应用包括基因表达定量测定和使用荧光 5' 核酸酶试验检测单核苷酸多态性 (SNP)。

灵活性
7900HT 系统是一个通用研究工具,可以满足任何实时荧光定量 PCR 需求。用户可互换的热循环模块规格可使您选择适合您项目的规格,使用行业标准 96 和 384 孔规格以及新型 384 孔 TaqMan™ 低密度阵列和新 Fast 96 孔模块,将运行时间从 2 小时缩短至约 30 分钟。简单的自动化升级路径允许您添加功能以满足通量需求。Fast PCR 选项可将运行时间从 2 小时缩短至约 30 分钟。

功能强大的软件
使用两种自动化软件工具(板实用程序和自动化控制器),大多数基因表达和 SNP 基因分型工作流程对于高通量应用程序是完全自动化的,并且仅需很少的用户干预。您现在可以处理 5,000 个 384 孔板(384 个标志),相当于 192 万个基因型。使用相对定量测定 (RQ) 管理器,您还可以处理 200 个 384 孔板(每个多通路内源性对照具有一式四份数据点的 96 个检测器),相当于 153,600 个数据点。SNP Manager 和 RQ Manager 完全集成到一个完整的企业版系统中,通过同时分析更多的平板,突破了高通量 SNP 和基因表达研究中的数据分析瓶颈。这可减少手动操作分析时间并简化数据分析工作流程。

仅供研究使用。不适用于诊断用途。
仅供科研使用。不可用于诊断程序。
规格
已校准染料SYBR™ Green I、ROX™、NED™、JOE™、TAMRA™、VIC™、FAM™、TET™
检测方法SYBR,引物探针检测
尺寸72 cm (W) x 84 cm (D) x 64 cm (H)(抽屉打开时)
动态范围高达9个对数级线性动态范围
适用于(设备)7900HT Fast 系统、AB 7900 HT 384 孔系统
产品规格384 孔板
高通量能力兼容高通量应用,多通路(技术)
光学延长寿命 488 nm 氩离子激光激发源,无滤光片(CCD 用作光谱仪,连续检测 500-650 nm)
参比荧光染料ROX(单独管)、无 ROX、其他惰性参比染料、ROX(预混合)
数量1 instrument
反应速度快速
反应体积范围5-20 µl
运行时间52分钟(384孔,快速模式),<2 小时(384孔,标准模式)
样品变温速率快速模式:± 2.2°C⁄s,标准模式:± 1.6°C⁄s
灵敏度低至10拷贝
温度范围(公制)4 至 100°C
热量准确度显示温度 ± 0.25°C
热循环系统基于 Peltier 的系统
热量均匀性±0.5°C
通量每天 ≥5,000 次实时 PCR 反应(384孔模块和自动化配件),每小时 ≥10,000 次终点 PCR 反应(384孔模块和自动化配件)
重量114 kg(带自动化配件)
模块规格可互换
容量多达84个384孔板
Unit SizeEach
内容与储存
1台带有384孔区块模块和自动化配件的仪器

常见问题解答 (FAQ)

What is the threshold on the Applied Biosystems real-time PCR instruments and how do I set it?

The threshold is the numerical value assigned for each run that reflects the average dRn (change in fluorescence) during the initial cycles of PCR (baseline). The threshold is set by determining a statistically significant point (or value) above the baseline. You can manually adjust the threshold should you desire, to the place in the geometric phase where your replicates are tightest. For more information on how to manually set a threshold, please refer to the tutorial entitled Data Analysis on the ABI PRISM 7700: Setting Baselines and Thresholds (P/N 4370923). While this tutorial is based on the ABI PRISM 7700 system, the concepts are still valid for the Applied Biosystems real-time PCR instruments. You can search the document on our website by using the part number above as the keyword.

If you choose not to manually adjust the baselines and thresholds, the SDS software on the Applied Biosystems real-time PCR instruments has an Auto Ct algorithm that can be used to automatically generate baseline and threshold values for individual detectors. The algorithm calculates baseline and threshold parameters for a detector based on the assumption that the data exhibits the "typical" amplification curve. Experimental error (i.e. contamination, pipetting inaccuracies) can produce amplification curves that deviate significantly from a typical amplification curve. The data from these irregularities can affect the Auto Ct algorithm by causing it to generate incorrect baseline and threshold parameters for the associated detector. Therefore, Applied Biosystems recommends that after analysis of experimental data, you review all baseline and threshold parameters determined by the Auto Ct algorithm.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)?

It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments:
• 7900HT Fast Real-Time PCR System
• 7500/7500 Fast Real-Time PCR System
• StepOne/StepOne Plus Real-Time PCR System
• ViiA 7 Real Time PCR System
• QuantStudio 1 Real-Time PCR System
• QuantStudio 3 & 5 Real-Time PCR System
• QuantStudio 6 & 7 Flex Real-Time PCR System
• QuantStudio 6 & 7 Pro Real-Time PCR Systems
• QuantStudio 12K Flex Real-Time PCR system

Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x):
1. Open the data file in the Design & Analysis Software (v2.x).
2. Click on Actions.
3. Click on "Standard Curve Analysis Setting".
4. Select "External Standard Curves" and click on "Import".
5. Browse to the proper .csv file and click "Open".
6. Click "Apply".

What is the difference between a Fast 96-well and standard 96-well block?

There are two main differences between a Fast 96-well and standard 96-well block for the Applied Biosystems real-time PCR systems:
• Reaction well volume: The Fast 96-well blocks have 0.1 mL reaction wells and are compatible with 0.1 mL plates and tubes. The standard 96-well blocks have 0.2 mL reaction wells and are compatible with 0.2 mL plates and tubes.
• Ramp rate: The fast 96-well blocks have a higher maximum ramp rate than standard blocks for the same real-time PCR system. However, both the fast 96-well and standard 96-well blocks are capable of running standard and fast chemistry.

Note: For newer instruments (QuantStudio 3 and 5 Real-Time PCR Systems and later) "0.1 mL" and "0.2 mL" are being used in place of "fast" and "standard" as designations for the two 96-well block formats.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What qPCR instruments are compatible with Thermo Fisher Cloud?

All our real-time PCR instruments are compatible with Thermo Fisher Cloud Apps except the 7500 Fast instrument where the eds files alone are compatible with Thermo Fisher Cloud Apps.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.