基质标准品试剂盒，BigDye™ Terminator v3.1，适用于 31xx/3500/SeqStudio™/SeqStudio™ Flex
基质标准品试剂盒，BigDye™ Terminator v3.1，适用于 31xx/3500/SeqStudio™/SeqStudio™ Flex
Applied Biosystems™

基质标准品试剂盒，BigDye™ Terminator v3.1，适用于 31xx/3500/SeqStudio™/SeqStudio™ Flex

本试剂盒含有两管标准品，用于生成在 31xx、3500、SeqStudio 和 SeqStudio Flex 系列基因分析仪上分析 BigDye Terminator了解更多信息
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货号数量
43369741 个试剂盒
货号 4336974
价格（CNY)
2,335.00
Each
添加至购物车
数量:
1 个试剂盒
价格（CNY)
2,335.00
Each
添加至购物车
本试剂盒含有两管标准品，用于生成在 31xx、3500、SeqStudio 和 SeqStudio Flex 系列基因分析仪上分析 BigDye Terminator v3.1 标记 DNA 片段时所需的光谱。每管均含有四种不同颜色的荧光染料标记 DNA 片段混合物（混悬于 1X TE 缓冲液中）。
仅供科研使用。不可用于诊断程序。
规格
适用于（应用）测序
适用于（设备）31xx 系列基因分析仪、SeqStudio™ 基因分析仪、3500 系列基因分析仪、SeqStudio Flex 系列基因分析仪
标签或染料ROX，TAMRA™，罗丹明 110，罗丹明 6G
产品线BigDye Terminator, BigDye
产品类型基质标准品
数量1 个试剂盒
运输条件湿冰
产品规格试剂盒
Unit SizeEach
内容与储存
在 2–8°C 下避光储存。切勿冷冻。

常见问题解答 (FAQ)

When I started my run on the Applied Biosystems 3500/3500xL Genetic Analyzer, an error occurred: "Unstable Electrophoresis current detected, check for air bubbles" but I don't see any bubbles. What else can cause this?

Some of the other causes of an Unstable Electrophoresis current detected error message are:

1.Leak on the system
2. Polymer that:
-Has expired
-Has been left on the instrument for more than the recommended time
-Is a mixture of expired polymer and non-expired polymer
3. Running Buffer that was:
-On the instrument longer than 2 weeks
-Not filled to the fill line or evaporated below the fill line
4. An arcing event that was not cleaned afterwards using the water wash wizard
5. Not performing regular maintenance on the instrument
6. Hardware issues

Inspect the system for leaks. If you do not see any leaks on the system, perform the wash the pump chamber and channels wizard using the conditioning pouch and place fresh, non-expired polymer and fresh Anode and Cathode buffer containers. If the problem persists, a service call may be required.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.

Do I need to replace the buffer every 14 days if the Applied Biosystems 3500/3500xL Genetic Analyzer is not in use?

If the instrument is not in use, it is not necessary to replace the buffer every 14 days. However, the buffer level needs to remain at the fill line to prevent the capillary or array from drying out. Top off the buffer volume with water if necessary. Replace the anode and cathode buffer containers prior to starting a new run.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.

How do I make a spectral standard for the Applied Biosystems 3130 series Genetic Analyzer?

The spectral calibration on the Applied Biosystems 3130 series systems can be performed by either using a Matrix Standard or a Sequencing Standard. The Matrix Standards will give 4 peaks, each of a different color whereas the Sequencing Standard is pre-sequenced and cleaned DNA that is dried down and just needs to be resuspended.

To make up the Matrix Standard:

  1. Vortex the Matrix Standard tube for 1 min at full speed and pulse spin in a microcentrifuge.
  2. Dilute the Matrix Standard in Hi-Di Formamide in a 1.5 mL microcentrifuge tube according to the following dilutions:
      For 4 capillary arrays
    • 36 cm and 80 cm arrays: mix 2.5 µL of Matrix Standard with 47.5 µL of Hi-Di Formamide.
    • 50 cm array: mix 2.5 µL of Matrix Standard with 97.5 µL of Hi-Di Formamide.

      For 16 capillary arrays
    • 36 cm and 80 cm arrays: mix 10 µL of Matrix Standard with 190 µL of Hi-Di Formamide.
    • 50 cm array: mix 5 µL of Matrix Standard with 195 µL of Hi-Di Formamide.
  3. Mix thoroughly and pulse spin in a microcentrifuge.
  4. Denature at 95 degrees C for 2 min and dispense 10 µL of reagent into wells A1–H12 (16 capillaries) or A1–D1 for 4 capillaries on a 96-well plate.
  5. Briefly spin to ensure there are no air bubbles at the bottom of the well.
  6. Set up and run the spectral calibration as per the Applied Biosystems 3130/3130xl Genetic Analyzers Getting Started Guide


More information on making up the Matrix Standards can be found in the BigDye Terminator v.3.1 Matrix Standard Kit for 3130 and 3100 Series Systems Product Insert or the BigDye Terminator v.1.1 Matrix Standard (Dye Set E) for 3100, 3130, and 3500 Series Genetic Analyzers Product Insert

To make up the Sequencing Standard:
  1. Resuspend one tube of the Sequencing Standard with 170 µl of Hi-Di Formamide.
  2. Vortex at full speed for 1 min and pulse spin in a microcentrifuge.
  3. Denature at 95 degrees C for 2 min and dispense 10 µL of reagent into wells A1–H12 (16 capillaries) or A1–D1 for 4 capillaries on a 96-well plate.
  4. Briefly spin to ensure there are no air bubbles at the bottom of the well.
  5. Set up and run the spectral calibration as per the Applied Biosystems 3130/3130xl Genetic Analyzers Getting Started Guide


Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Instruments Support Center.