7900HT TaqMan™ 低密度阵列仪器验证 RNase P 试剂盒
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Applied Biosystems™

7900HT TaqMan™ 低密度阵列仪器验证 RNase P 试剂盒

7900HT TaqMan® 低密度阵列仪器验证 RNase P 试剂盒用于通过 TaqMan® 低密度阵列模块升级包验证 Applied Biosystems®了解更多信息
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货号数量
43514681 kit
货号 4351468
价格(CNY)
31,556.00
Each
添加至购物车
数量:
1 kit
价格(CNY)
31,556.00
Each
添加至购物车
7900HT TaqMan® 低密度阵列仪器验证 RNase P 试剂盒用于通过 TaqMan® 低密度阵列模块升级包验证 Applied Biosystems® 7900HT Fast 实时荧光定量 PCR 系统的性能。7900HT TaqMan® 低密度阵列仪器验证 RNase P 试剂盒包含一个空 TaqMan® 低密度阵列和 8 瓶 RNase P 检测试剂,以检测和定量测定人 RNase P 基因(编码 RNase P 酶 RNA 部分的单拷贝基因)的基因组拷贝数。每瓶都包含预混合试剂(1 X TaqMan® 通用 PCR 预混液、RNase P 引物和 FAM™ 染料标记探针)和模板。必须证明 7900HT TaqMan® 低密度阵列仪器验证 RNase P 试剂盒能够区分 5,000 和 10,000 个基因组当量且在运行后续单个孔样品时具有 99.7% 的置信水平。为确保获得准确的结果,Applied Biosystems® 建议您每六个月验证一次仪器的性能。
仅供科研使用。不可用于诊断程序。
规格
已校准染料FAM
校准类型RNase P 校准,仪器验证板
检测方法荧光
适用于(应用)实时 PCR (qPCR)
适用于(设备)7900 HT LDA 系统
产品线TaqMan
数量1 kit
产品类型仪器验证试剂盒
Unit SizeEach
内容与储存
包含一个空 TaqMan™ 低密度阵列和 8 瓶 RNase P 检测试剂。在 -15 到 25°C 下储存。避光储存

常见问题解答 (FAQ)

When do I need to calibrate the 7900HT Fast Real-Time PCR System?

Recommended Maintenance Schedule

Power on/off the computer controlling the instrument: Weekly
Check computer disk space. If necessary, archive or back up your experiment files and instrument settings: Weekly
Run disk cleanup and disk defragmentation: Every month
Background calibration: Every month
Perform an instrument self test: Every month
Pure dye calibrations: Every 6 months
RNaseP instrument verification: After the instrument has been moved, or as needed to verify instrument performance

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Can I use my 7900HT System RNase P instrument verification plates with both the 7900HT System and the QuantStudio 6 and 7 Flex Real-Time PCR Systems?

Only the TaqMan RNase P Instrument Verification Plate, Fast 96-well (for 0.1 mL block) (Cat. No. 4351979) is compatible with both the 7900HT System and the QuantStudio 6 and 7 Flex Real-Time PCR Systems. The following RNase P instrument verification plates can be used on the 7900HT System, but not on the QuantStudio 6 and 7 Flex Real-Time PCR Systems:
• TaqMan RNase P Instrument Verification Plate, 96-well (Cat. No. 4310982) (for 96-well standard block)
• TaqMan RNase P Instrument Verification Plate for 7900HT System, 384-well (Cat. No. 4323306) (for 384-well block)
• 7900HT TaqMan Low Density Array Instrument Verification RNase P Kit (Cat. No. 4351468) (for TaqMan Array Card block)

The following RNase P instrument verification plates can be used on the QuantStudio 6 and 7 Flex Real-Time PCR Systems, but not on the 7900HT System:
• TaqMan RNase P Instrument Verification Plate for ViiA7 System, 96-well (Cat. No. 4432382) (for 96-well standard block)
• TaqMan RNase P Instrument Verification Plate for ViiA7 System, 384-well (Cat. No. 4455280) (for 384-well block)
• ViiA7 Array Card RNase P Verification Kit (Cat. No. 4432265) (for TaqMan Array Card block)

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What is the difference between a Fast 96-well and standard 96-well block?

There are two main differences between a Fast 96-well and standard 96-well block for the Applied Biosystems real-time PCR systems:
• Reaction well volume: The Fast 96-well blocks have 0.1 mL reaction wells and are compatible with 0.1 mL plates and tubes. The standard 96-well blocks have 0.2 mL reaction wells and are compatible with 0.2 mL plates and tubes.
• Ramp rate: The fast 96-well blocks have a higher maximum ramp rate than standard blocks for the same real-time PCR system. However, both the fast 96-well and standard 96-well blocks are capable of running standard and fast chemistry.

Note: For newer instruments (QuantStudio 3 and 5 Real-Time PCR Systems and later) "0.1 mL" and "0.2 mL" are being used in place of "fast" and "standard" as designations for the two 96-well block formats.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.