StepOne™ 实时荧光定量 PCR 系统光谱校准试剂盒(48 孔)
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Applied Biosystems™

StepOne™ 实时荧光定量 PCR 系统光谱校准试剂盒(48 孔)

这种易于使用的试剂盒可在 StepOne™ 实时荧光定量 PCR 系统上建立适用于 FAM™/SYBR™ Green I、VIC™/JOE™了解更多信息
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货号数量
43714331 kit
货号 4371433
价格(CNY)
8,699.00
Each
添加至购物车
数量:
1 kit
价格(CNY)
8,699.00
Each
添加至购物车
这种易于使用的试剂盒可在 StepOne™ 实时荧光定量 PCR 系统上建立适用于 FAM™/SYBR™ Green I、VIC™/JOE™ 和 ROX™ 染料的纯染料光谱和多组分值。

StepOne™ 实时荧光定量 PCR 系统光谱校准试剂盒的特点包括:

• 预装染料(FAM™/SYBR™ Green I、VIC™/JOE™ 和 ROX™)的 48 孔光学反应板,使您可以快速轻松地校准 StepOne™ 实时荧光定量 PCR 系统
• 易于使用,可准确辨别反应混合物中单个染料的荧光信号
• 包括惰性参比染料 I 标准品,用于所有实时和终点反应中的信号归一化

Applied Biosystems™ StepOne™ 实时荧光定量 PCR 系统光谱校准试剂盒含有一个背景板以及三个预装 48 孔快速光学反应板(配有五种染料标准品)。
仅供科研使用。不可用于诊断程序。
规格
已校准染料FAM、JOE、ROX、VIC、SYBR™ Green
校准类型光谱校准
检测方法荧光
适用于(应用)实时 PCR (qPCR)
适用于(设备)StepOne™,快速模式,StepOne™,标准模式
产品线StepOne
数量1 kit
运输条件干冰
产品类型光谱校准试剂盒
Unit SizeEach
内容与储存
随附三个 48 孔反应板—一个背景板和两个含染料标准品的板(板 I 和 II)。板 I 含 FAM、JOE 和 ROX;板 II 含 SYBR™ Green I 和 VIC。

于 -15 至 25°C 下储存。避光储存。

有效期:自交付日起 12 个月

常见问题解答 (FAQ)

What does "Error - Cannot calculate Pure Dye Matrix" mean?

This issue can occur if the run is interrupted. To prevent this from happening in the future, avoid ending the PCR with an infinite hold stage or interrupting the run. Please send the file to techsupport@thermofisher.com so that our software group can attempt to recover the data.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What does "Error - Fatal Error Number 0x8158" mean?

This is generally caused by incorrect placement of tube strips, resulting in a sample block that can't properly be lowered. We recommend contacting Instrument Services to have an engineer walk you through a manual drawer-lowering process during instrument power-up. To prevent this from happening in the future, place tubes toward the middle of the block or use a placeholder strip on the opposing side to counterbalance block pressure.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What does "Error - Java.lang.IllegalArgumentException: One of the raw spectra is null" mean?

This issue can occur if the “Start Run” button is clicked more than once or if illegal characters are used in the experiment/file name. You can recover the data yourself by overriding the calibration file with one that represents the calibration status of the instrument when you ran that particular file. Use the toolbar at the top to navigate: Analysis then Override Calibration then Use Calibration From Another File.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What does "Error - Cannot open the file because file contents are not recognized" mean?

This issue can occur if duplicate samples are added to the experiment, if illegal characters are used in the experiment/file name, or if the file extension was changed to .eds from a different extension.
Please send the file to techsupport@thermofisher.com to attempt recovery.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What does "Error - Analysis failed due to GExSession doesn't exist in context" mean?

This error means that the data collection was not turned on as appropriate. If this is inadvertently turned off, no data will be collected. Thus, the data are not retrievable in such a file.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.