TaqMan™ Sample-to-SNP™ 试剂盒 (20 mL 样品制备,50 mL PCR)
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Applied Biosystems™

TaqMan™ Sample-to-SNP™ 试剂盒 (20 mL 样品制备,50 mL PCR)

The Applied Biosystems TaqMan Sample-to-SNP Kit allows you to go from biological sample to genotyping results in less than one了解更多信息
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货号数量
44030871 kit
货号 4403087
价格(CNY)
63,263.00
Each
添加至购物车
数量:
1 kit
价格(CNY)
63,263.00
Each
添加至购物车

The Applied Biosystems TaqMan Sample-to-SNP Kit allows you to go from biological sample to genotyping results in less than one hour. It combines DNA extraction and TaqMan chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• Provides PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan GTXpress Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2–8°C significantly reduce assay setup time
• Validated with all types of TaqMan Genotyping assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 400 sample preparation reactions at 50 μL/reaction and 20,000 PCR reactions at 5 μL for each reaction.

仅供科研使用。不可用于诊断程序。
规格
适用于(设备)7300 系统、7500 Fast 系统、7500 系统、7900HT Fast 系统、GeneAmp 9700、StepOne™、快速模式、StepOne™、标准模式、Veriti 热循环仪、StepOne™ 标准模式、StepOne™ 快速模式
基因分型靶标SNP(未知或多种)、SNP(已知)
热启动内置热启动
反应次数4000次反应 (5 μL PCR)、4000次反应(每反应50 μL 样品制备)
参比荧光染料ROX(预混)
聚合酶AmpliTaq DNA 聚合酶
产品线样本至 SNP、TaqMan
产品类型PCR 试剂盒
数量1 kit
样品类型口腔样品、细胞培养物、带毛囊的头发、叶片穿孔或针、种子芯片、尾活检(小鼠)、尾活检(大鼠)、组织 (FFPE)、全血
技术SNP 基因分型(基于实时定量 PCR)
容量20 mL(样品制备)、50 mL (PCR)
检测方法引物-探针
PCR 方法qPCR
反应速度快速,标准
Unit SizeEach
内容与储存
内含裂解试剂 (2-8°C):20 mL 各裂解液和 DNA 稳定液;预混液 (15-25°C):50 mL 含 AmpliTaq™ Fast DNA 聚合酶(超纯)、dNTP、追踪染料和 ROX™ 染料的 2X 预混液。

保证的最短有效期为60天(打印于产品和检验报告上的确切有效期)。

常见问题解答 (FAQ)

When designing probes to perform Allelic Discrimination (SNP assays), are there any alternatives to using probes longer than 30bp in order to get the appropriate melting temperature?

Applied Biosystems TaqMan MGB (minor groove binder) probes would be appropriate for Allelic Discrimination/SNP Genotyping probe designs. TaqMan MGB probes have a minor groove binder at the end of the probe. This minor groove binder increases the Tm of probes, allowing the use of shorter probes. Consequently, the TaqMan MGB probes exhibit greater differences in Tm values between matched and mismatched probes, which provides more accurate allele discrimination.

For information on the design of TaqMan MGB probes for Allelic Discrimination/SNP assays using Primer Express software, please refer to our guide "Designing TaqMan MGB Probe and Primer Sets for Allelic Discrimination Assays Using Primer Express".