裂解试剂(2° 至 8°C):5 mL 各裂解液和 DNA 稳定液;预混液(15 ° 至 25°C):含 AmpliTaq™ Fast DNA 聚合酶 (UP)、dNTP、追踪染料和 ROX™ 染料的 1 mL 2X 预混液。
保证的有效期下限为60天(确切有效期印于产品和检验报告上)。
常见问题解答 (FAQ)
When designing probes to perform Allelic Discrimination (SNP assays), are there any alternatives to using probes longer than 30bp in order to get the appropriate melting temperature?
Applied Biosystems TaqMan MGB (minor groove binder) probes would be appropriate for Allelic Discrimination/SNP Genotyping probe designs. TaqMan MGB probes have a minor groove binder at the end of the probe. This minor groove binder increases the Tm of probes, allowing the use of shorter probes. Consequently, the TaqMan MGB probes exhibit greater differences in Tm values between matched and mismatched probes, which provides more accurate allele discrimination.
For information on the design of TaqMan MGB probes for Allelic Discrimination/SNP assays using Primer Express software, please refer to our guide "Designing TaqMan MGB Probe and Primer Sets for Allelic Discrimination Assays Using Primer Express".