TaqMan™ 阵列,人类 MAPK 通路,快速 96 孔
TaqMan™ 阵列,人类 MAPK 通路,快速 96 孔
Applied Biosystems™

TaqMan™ 阵列,人类 MAPK 通路,快速 96 孔

针对特定的生物过程、通路或疾病状态,这些 96 孔板已预先配置了较合适的 TaqMan® Gene Expression Assay。每个板的孔中都含有预设的试剂和内参(干了解更多信息
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货号数量
44187381 x 96-well plate
货号 4418738
价格(CNY)
2,663.00
Each
添加至购物车
数量:
1 x 96-well plate
价格(CNY)
2,663.00
Each
添加至购物车
针对特定的生物过程、通路或疾病状态,这些 96 孔板已预先配置了较合适的 TaqMan® Gene Expression Assay。每个板的孔中都含有预设的试剂和内参(干),可在一次简单实验中精确评估整个基因标签。TaqMan® 阵列 96 孔人类 MAP 激酶通路板中的检测板可靶向以下四组主要哺乳动物 MAPK 基因:1) ERK(细胞外信号调节激酶);2) p38 MAPK;3) JNK(c-Jun NH2 - 末端激酶);和 4) ERK5(或 BMK 级联)。板上还包含来自 MAP2K (MAPKK)、MAP3K (MAPKKK) 和双重特异性蛋白激酶 MAPKK (DUSP) 组的基因。该板中的其他激酶组包括:周期素依赖性蛋白激酶 (CDK)、MAP 激酶相互作用丝氨酸⁄苏氨酸激酶 (MNK)、p21 活化激酶 (PAK) 和核糖体 S6 激酶 (RSK)。还有包括一些与信号转导、转录调控、细胞周期调控和癌症相关的 MAPK 靶标。
仅供科研使用。不可用于诊断程序。
规格
形式干燥
包括适用于一次 10 uL 反应的 TaqMan 检测
内部探针修改MGB(小沟结合物)(3'),非荧光淬灭基团 (3'),FAM (5')
标签或染料FAM
产品线TaqMan
数量1 x 96-well plate
反应速度快速
研究类别细胞周期&增殖、信号转导、生物标记物相关通路、开发、凋亡、肿瘤学
类型阵列板
产品规格96 阵列板
种属
Unit SizeEach
内容与储存
每个 TaqMan™ 阵列板孔包含进行一次 10 µL 反应所需的一份 TaqMan™ 检测试剂。可于室温或 4°C 下储存这些板。

常见问题解答 (FAQ)

What mastermix can I use for running samples on TaqMan Gene Signature Array Plates?

We recommend that for standard (20 µL) TaqMan Array Plates, you use either the Universal Master Mix (I or II) or the Gene Expression MasterMix. For fast (10 µL) TaqMan Array Plates, use the Fast Universal Master Mix. The Fast Advanced Master Mix can also be used for either plate type, but we recommend following the protocol in this manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0025706_TaqManFastAdvMM_UG.pdf).

What is the minimum amount of TaqMan array plates I need to purchase to submit an order?

The minimum amount of TaqMan Array plates needed to submit an order is based on the plate type:
• Inventoried TaqMan array plates: 1 plate
• TaqMan Flexible Content panel array plates: 6 plates
• Custom TaqMan array plates: 6 plates
• Specialty TaqMan array plates: 10-20 plates (depends on assay format and configuration). Please contact our Specialty Plates team at Specialty_Plates@thermofisher.com for details.

Can our TaqMan Array Plates be run on non-Applied Biosystem real-time PCR instruments?

We recommend using our Plastics Selection Tool to verify which plates are compatible with your instrument model, https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/plastics-selection-guide.html.

Based on our testing, the 96-well TaqMan Array Plates can be used on the following non-Applied Biosystem real-time PCR instruments:
• Standard (0.2mL) 96-well Plates: Bio-Rad IQ5
• Fast (0.1mL) Plates: Bio-Rad CFX Connect and Bio-Rad CFX96 Touch

How do I obtain TaqMan documentation files for my TaqMan array plates?

Beginning in April 2016, TaqMan documentation files for TaqMan Array Plates (assay information files (AIF), experimental setup files, and plate layout files) can be downloaded from the Thermo Fisher Scientific website at https://www.thermofisher.com/taqmanfiles. To obtain your TaqMan documentation files:
• Select the appropriate product type.
• Enter the information being requested for the product type.
• Fixed TaqMan array plates: Enter the Part number and Batch number
• TaqMan Flexible Content panel array plates/Custom TaqMan array plates: Enter the Sales Order number and Batch number
• Click Submit.