适用于(设备)7000系统,7300系统,7500 Fast 系统,7500系统,7700系统,7900 HT 系统、 AB 7900 HT 384 孔系统,StepOne™,标准模式,StepOnePlus™,标准模式
产品规格管
热启动内置热启动
反应次数500 次反应
参比荧光染料ROX(预混)
聚合酶AmpliTaq Gold DNA 聚合酶
产品线路径-ID
产品类型一步法 RT-PCR 试剂盒
数量500 reactions
逆转录酶ArrayScript™
样品类型总 RNA,病毒 RNA,mRNA
足够用于500 次反应
检测方法引物-探针
适用于(应用)病原检测, 病毒检测
形式液体
PCR 方法一步法 RT-qPCR, 多重 qPCR
反应速度标准
Unit SizeEach
内容与储存
•7 mL 2X 多重 RT-PCR 缓冲液 • 1.4 mL 10X 多重酶混合物,其中包括AmpliTaq Gold 和 ArrayScript • 25 mL 无核酸酶水 • 试剂盒 -20°C 储存;无核酸酶水可在 -20°C、4°C 或室温下储存
保质期至少60天(确切的有效期已印在产品和分析证书上)。
常见问题解答 (FAQ)
What can I do to improve the sensitivity of my qPCR assay?
If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:
- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)
How do I set the baseline for my qPCR experiment?
Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).
How do I set the threshold for my qPCR experiment?
In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).
I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?
There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.
I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?
There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.