TaqMan™ MicroRNA Assay

Applied Biosystems TaqMan MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan Assay chemistry. A simple, two-step protocol requires only reverse transcription with an miRNA-specific primer, followed by real-time PCR with TaqMan probes.

TaqMan MicroRNA Assays are:

• Highly specific—quantitate only mature miRNAs, not precursors
• Sensitive—conserve limited samples, requires only 1–10 nanograms of total RNA or equivalent
• Fast, simple and scalable—two-step quantitative RT-PCR assay provides high-quality results in less than three hours

Our TaqMan MicroRNA Assay catalogue is kept up to date with the Sanger database to provide comprehensive coverage with the most current annotation.

Availability and sizes

Made-to-Order assays are available in four different sizes and usually ship within two weeks. Inventoried assays are prepackaged in Small size and are ready to ship. Inventoried assays may be purchased in larger sizes by ordering them as Made to Order.

Species

TaqMan MicroRNA Assays are available for a range of species including human, mouse, rat, Drosophila, C. elegans, and Arabidopsis. We will continue to increase the number of miRNA assays for these species in alignment with the Sanger miRBase Registry.

Assay Components

TaqMan MicroRNA Assays are delivered in two tubes: one containing the RT primer, the other containing the specific pre-formulated TaqMan Assay (TaqMan probe and PCR primer set).

Pre-formulated assay

• Forward primer: 1500 nM
• Reverse primer: 700 nM
• FAM-MGB probe: 200 nM
• RT sequences: 50 nM

TaqMan miRNA Assay selection guide

TaqMan MicroRNA Assays

• Description: TaqMan MicroRNA Assays employ a novel target-specific stem–loop primer during cDNA synthesis to produce a template for real-time PCR
• RT chemistry: miRNA-specific RT
• Throughput: Best for 1–10 targets
• Coverage: 205 species available, coverage for miRBase v.21

TaqMan Advanced miRNA Assays

• Description: TaqMan Advanced miRNA Assays employ a universal RT step for a streamlined workflow, and a universal miR-Amp step to enable highly sensitive detection by real-time PCR
• RT chemistry: Universal RT
• Throughput: Best for >10 targets
• Coverage: All human, mouse, and rat miRNAs; coverage for miRBase v.24