Megaplex™ 引物池，Rodent Pools B v3.0

Megaplex™ Primer Pools, Rodent Pool B v3.0 is available under a single part number containing both the Megaplex™ RT Primers了解更多信息

货号	数量
4444752	50 reactions

货号 4444752

价格（CNY)

16,933.00

添加至购物车

50 reactions

价格（CNY)

16,933.00

添加至购物车

Megaplex™ Primer Pools, Rodent Pool B v3.0 is available under a single part number containing both the Megaplex™ RT Primers and Megaplex™ PreAmp Primers, Rodent Pools B v3.0. Megaplex™ RT Primers, Rodent Pool B contains RT primers for 306 and 135 unique microRNAs for mouse and rat respectively plus 4 species specific controls. For use when assay sensitivity is of the utmost importance, Megaplex™ PreAmp Primers significantly enhance the ability to detect low expressed microRNAs enabling the generation of a comprehensive expression profile using far less sample, as low as 1 ng of input total RNA. The microRNA targets represented in this pool tend be less well characterized, more narrowly expressed and⁄or expressed at a lower level compared to Rodent Pool A. Rodent Pool B is intended for use with the TaqMan Rodent MicroRNA B Array v3.0, however, it can also be used to prepare cDNA for real-time analysis using the individual TaqMan® MicroRNA Assays.

仅供科研使用。不可用于诊断程序。

反应次数50 次反应

引物RT 引物、PreAmp 引物

产品线MegaPlex，TaqMan

产品类型引物池

数量50 reactions

种属大鼠、小鼠

足够用于50 次反应

适用于（应用）miRNA 分析

高 GC PCR 扩增效果低

PCR 方法qPCR

Unit SizeEach

内容与储存

储存在 -20°C 下。

常见问题解答 (FAQ)

Can Ct's greater than a cut-off be considered valid results?

Yes they can. However, it is important to recognize the true linearity and detection limits of your assay: Ct values above the cut-off can indicate non-specific amplification, unless your NTC is a true- no-target control, and you have run a statistically significant number of replicates. Any results with Ct above the recommended cut-off need to be validated with individual assays on plates.

What is a good Ct cut-off for the TaqMan MicroRNA Array Cards and TaqMan Advanced miRNA Array Cards? In other words, beyond what Ct should I not trust the data?

The typical Ct cut-off on TaqMan Array Cards is 32, which is equivalent to Ct 35 on a plate (10 µl reaction). Previous studies show that if you use pre-amplification, a Ct cut-off of 29 or 30 can be used to reduce numbers of false positives (see Technical Note Optimized protocols for human or rodent microRNA profiling with precious samples). To ensure that you have selected a correct cut-off, you should run replicates of the same sample and use Ct cut-off before you see an increase in the Standard Deviation.