There is a specific ERCC_Analysis Plugin for Torrent Suite Software. Please see the ERCC_Analysis Plugin User Bulletin (Publ. No. 4479068) for detailed information.
Where can I find the ERCC concentrations?
Please go to the ERCC RNA Spike-In Mix (Cat. No. 4456740) or ERCC Ex-Fold Spike-In Mix (Cat. No. 4456739) product page on www.thermofisher.com. Downloadable files are under the manuals section heading, click on Manual: ERCC Controls Analysis: ERCC RNA Spike-In Control Mixes.
Is there an ERCC-like product for bacteria or small RNA (or other non-polyA RNA species)?
No.
What is the difference between the ERCC RNA Spike-In Mix (Cat. No. 4456740) and the ERCC Ex-Fold Spike-In Mix (Cat. No. 4456739)?
The ERCC RNA Spike-In Mix (Cat. No. 4456740) includes Spike-In Mix 1 alone; it can be used to assess platform dynamic range and lower limit of detection. The ERCC Ex-Fold Spike-In Mix (Cat. No. 4456739). includes both Spike-In Mix 1 and Spike-In Mix 2. In addition to the performance measurements described above for the ERCC RNA Spike-In Mix, this kit can be used to assess the accuracy of measurements of differential gene expression on your platform. Please see the ERCC RNA Spike-In Control Mixes User Guide (Pub. No. 4455352) for detailed information.
What are the ERCC RNA Spike-In Control mixes (Cat. No. 4456740 & 4456739)?
The ERRC RNA Spike-In Control mixes are a set of external RNA controls developed by the External RNA Controls Consortium (ERCC) and NIST (National Institute of Standards and Technology). The set is comprised of 92 synthetic, unlabeled polyadenylated transcripts of various lengths for determining the limit of detection (LOD) and the dynamic range for mRNA expression studies (in eukaryotes). The spike-in mix should be added to the RNA sample after isolation but before making the library. For more information, please refer to ERCC RNA Spike-In Control Mixes User Guide (Publ. No. 4455352) and ERCC_Analysis Plugin User Bulletin (Publ. No. 4479068).