适用于 QuantStudio™ 12K Flex 系统的 HRM 软件模块

该软件模块扩展了 QuantStudio™ 12K Flex 软件的功能，允许在 PCR 扩增子上进行高分辨率熔解 (HRM) 分析。高分辨率熔解了解更多信息

货号	数量
4472054	1 kit

货号 4472054

价格（CNY)

1 kit

该软件模块扩展了 QuantStudio™ 12K Flex 软件的功能，允许在 PCR 扩增子上进行高分辨率熔解 (HRM) 分析。

高分辨率熔解 (HRM) 分析是一种新型 PCR 后分析方法，用于识别核酸序列中的基因变异。该方法简单、快速、基于 PCR 熔解（解离）曲线技术，并且最近提供改进的双链 DNA (dsDNA) 结合染料以及下一代实时荧光定量 PCR 仪器和分析软件。HRM 分析能够根据 DNA 序列的组成、长度、GC 含量或链互补区分 DNA 序列。

仅供科研使用。不可用于诊断程序。

适用于（设备）QuantStudio™ 12 k Flex

许可证10 个席位

操作系统Windows 7

产品线QuantStudio

数量1 kit

Unit SizeEach

常见问题解答 (FAQ)

What is the difference between continuous melt curves and step and hold melt curves on the Applied Biosystems Real-Time PCR Instruments?

Continuous melt curve: increases the temperature by the ramp increment (degrees C/sec) so that the temperature will change at a constant rate set in the protocol.
Step and Hold melt curve: increases the temperature by the ramp increment (degrees C) and then holds at that temperature for the time specified by the user.

The amount of data collected will depend on the filter(s) selected. The more filters you use, the longer the acquisition will take and the fewer data points will be collected.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

What type of Real-Time PCR (Sequence Detection System) information is available from Applied Biosystems?

Tutorials demonstrating introductions to Real-Time PCR, guidelines for designing real-time assays using Primer Express software, and various instrument set-ups can be found under Life Technologies University on our website. User bulletins, manuals and Product inserts, protocols can be found as downloadable pdf files on our Technical Resources page.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.