Ion AmpliSeq™ Cancer Hotspot Panel v2

A DNA fragment library is constructed from whole genomic DNA and is commonly used for whole genome resequencing or de novo sequencing. Briefly, the whole genomic DNA is fragmented or sheared, ligated with Ion-specific adapter sequences, and then size-selected for the library fragments of the desired length.

Amplicon libraries are constructed from PCR-amplified DNA fragments and are used for targeted sequencing (e.g., investigating variants at known genomic locations). There are two types of amplicon libraries, short and long.

A short amplicon library contains DNA fragments (targets) with lengths that are compatible with the Ion template preparation kits without any further shearing or fragmentation during library preparation. Additionally, no size-selection step is required, as the amplicons are already within the desired size range.

A long amplicon library contains DNA fragments (targets) with lengths that are longer than those compatible with the Ion template kits and requires further shearing or fragmentation during library preparation. The library preparation protocol for long amplicons is similar to fragment libraries.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

The Ion Library Equalizer Kit (Cat. No. 4482298) is recommended for use with Ion AmpliSeq libraries and provides an alternative to library quantification methods by using bead-based technology to normalize the final library concentration to ~100 pM.

The Ion Library Equalizer Kit is fast and cost-effective compared to traditional quantification methods; however, it may be not be the right choice for all users. Briefly, the library is amplified with the Ion Equalizer Primers, captured onto Equalizer Beads, and the normalized library is eluted from the beads using a specially formulated Equalizer Elution Buffer. The final library is normalized to ~100 pM, but there is no quality control information (e.g., measured concentration or size distribution) that can be obtained, which is possible if using the recommended library quantification kits: Ion Library Quantitation Kit (qPCR), Qubit dsDNA HS Assay Kit (Qubit 2.0 Fluorometer), or Agilent High Sensitivity DNA Kit (Agilent Bioanalyzer instrument).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Detailed information regarding the variants targeted for each of the panels can be found at www.ampliseq.com. Under the Panels tab, find your panel of interest and press the “Download panel files” buttons. The ZIP archive will contain CSV files that include links to the COSMIC entries for each mutation on the cancer-related panels.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Please visit the Ion AmpliSeq Designer website (www.ampliseq.com) and log in with your Thermofisher username and password. Select the “Help” tab to review training videos and documentation, including pipeline details, troubleshooting guides, and frequently asked questions.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

For template preparation, Ion AmpliSeq libraries are diluted to 100 pM, and the volume required for template preparation will vary depending on the template preparation kit used. Please see the Ion AmpliSeq Library Preparation User Guide for details regarding library dilutions and input into template preparation.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.