胰岛素-转铁蛋白-硒-丙酮酸钠 (ITS-A) (100X)

使用 ITS-A（胰岛素-转铁蛋白--硒-丙酮酸钠）补充剂作为基础培养基补充剂，以减少培养细胞所需的胎牛血清 (FBS) 的量。ITS-A 用于贴壁细胞培养。ITS-A 最初与了解更多信息

货号	数量
51300044	10 mL

货号 51300044

价格（CNY)

1,310.00

添加至购物车

10 mL

价格（CNY)

1,310.00

添加至购物车

使用 ITS-A（胰岛素-转铁蛋白--硒-丙酮酸钠）补充剂作为基础培养基补充剂，以减少培养细胞所需的胎牛血清 (FBS) 的量。ITS-A 用于贴壁细胞培养。ITS-A 最初与 RPMI 1640 和最低必需培养基 (MEM) 配合使用，现在通常与其他基础培养基配合使用，以在存在 2–4% FBS 的情况下支持多种细胞类型。我们还提供 ITS-G 和 ITS-X 补充剂。

使用 ITS-A 补充剂
胰岛素可促进葡萄糖和氨基酸摄取、脂肪形成、细胞内运输以及蛋白和核酸的合成。转铁蛋白是铁载体，也有助于降低氧自由基和过氧化物的毒性水平。亚硒酸盐是谷胱甘肽过氧化物酶和其他蛋白的一种辅因子，在培养基中用作抗氧化剂。丙酮酸钠是氨基酸、脂肪酸和胆固醇的前体；是许多生物合成途径中的重要中间体。提供完整配方。

cGMP 生产和质量体系
ITS-A 补充剂在位于纽约格兰德岛的符合 cGMP 要求的工厂生产。该工厂是在 FDA 登记的医疗器械生产商，且通过 ISO 13485 标准认证。

仅供科研使用。不可用于诊断程序。

细胞类型哺乳动物细胞

最大浓度100 X

培养类型哺乳动物细胞培养

数量10 mL

有效期18 个月

运输条件室温

产品类型转铁蛋白

无菌无菌过滤

加有添加剂丙酮酸钠

不加添加剂无酚红

Unit SizeEach

内容与储存

储存条件：2 至 8°C
运输条件：环境条件
有效期：自生产之日起 18 个月

常见问题解答 (FAQ)

What is the buffer composition of the various Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?

All ITS and ITS Select supplements are formulated in Earle's Balanced Salt Solution (EBSS).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?

ITS Select supplements are animal origin-free (AOF) versions of the ITS supplements offered by Thermo Fisher Scientific.

ITS Select supplements use recombinant versions of both proteins in the formulation: insulin and transferrin.

All other versions of ITS, those without the Select designation, utilize recombinant insulin and a human-derived holo transferrin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the individual concentrations of insulin, transferrin, and selenium in the Insulin-Transferrin-Selenium (ITS) (100X) supplements?

All ITS and ITS Select supplements have the following concentrations of their components:

Insulin: 1.0 g/L
Transferrin: 0.55 g/L
Selenium: 0.00067 g/L

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I store aliquots of Insulin-Transferrin-Selenium (ITS) (100X) supplements at -20 degrees C without compromising the integrity of the product?

We recommend storing all ITS and ITS Select supplements at 2-8 degrees C as there is a chance that the insulin will precipitate upon freezing and thawing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are all Insulin-Transferrin-Selenium (ITS) (100X) supplements offered by Thermo Fisher Scientific animal origin-free?

We offer both animal origin and animal origin-free versions of our ITS supplements. The animal origin-free product versions are referred to as ITS Select supplements.

Insulin-Transferrin-Selenium (ITS-G) (100X) (Cat. No. 41400045), Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) (100X) (Cat. No. 51500056), and Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X) (Cat. No. 51300044) are not animal origin-free.

Animal origin-free product versions are available for the ITS-G and ITS-X formulations: Insulin-Transferrin-Selenium (ITS-G) Select Supplement (100X) (Cat. No. A4000046501 and A4000046502) and Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) Select Supplement (100X) (Cat. No. A4000046401 and Cat. No. A4000046402.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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