eBioscience™ JC-1 线粒体膜电位染料
Invitrogen17万+抗体限时买二赠一,靶点广,灵活用!
Product Image
Invitrogen™

eBioscience™ JC-1 线粒体膜电位染料

JC-1 是一种膜可透过性染料,广泛用于在流式细胞分析和荧光显微镜检测中测定线粒体膜电位。这种染料可选择性进入线粒体,可在线粒体中随着膜电位的升高(电位值超过约 80-100 mV)可逆地改变颜色。这种特性源于膜偏振时可逆性形成的 JC-1 聚集体,当在波长了解更多信息
Have Questions?
货号数量
65-0851-385 mg
货号 65-0851-38
价格(CNY)
2,712.00
飞享价
Ends: 31-Dec-2025
3,604.00
共减 892.00 (25%)
Each
添加至购物车
数量:
5 mg
价格(CNY)
2,712.00
飞享价
Ends: 31-Dec-2025
3,604.00
共减 892.00 (25%)
Each
添加至购物车
JC-1 是一种膜可透过性染料,广泛用于在流式细胞分析和荧光显微镜检测中测定线粒体膜电位。这种染料可选择性进入线粒体,可在线粒体中随着膜电位的升高(电位值超过约 80-100 mV)可逆地改变颜色。这种特性源于膜偏振时可逆性形成的 JC-1 聚集体,当在波长 488 nm 下对其进行激发时,可导致发射光从 530 nm(即 JC-1 单体形式的发射波长)偏移至 590 nm(即 J-聚合体的发射波长)。可使用分别适用于 FITC 和 PE/藻红蛋白/罗丹明的滤光片检测两种颜色。JC-1 可用于定性(可观察到荧光发射从绿色偏移到橙色)和定量(可在两个滤光片组上利用荧光强度进行测定)分析。JC-1 可用于指示细胞凋亡是否开始。

分子量:652.23
激发峰值:515 nm
发射峰值:529 和 590 nm

JC-1 应在高质量新鲜打开的 DMSO (1 mg/mL) 中复溶。复溶后,应储存在 -20°C 下;避免冻融。

已报告应用
流式细胞术分析、显微镜检查
仅供科研使用。不可用于诊断程序。
规格
检测方法荧光
数量5 mg
子细胞定位线粒体
颜色橙色
发射515⁄529&590
适用于(设备)荧光显微镜, 流式细胞分析
产品线eBioscience
产品类型染料
Unit SizeEach

引用和文献 (7)

引用和文献
Abstract
A novel role for nuclear factor-erythroid 2 in erythroid maturation by modulation of mitochondrial autophagy.
Authors:Gothwal M, Wehrle J, Aumann K, Zimmermann V, Gründer A, Pahl HL
Journal:Haematologica
PubMed ID:27479815
'We have recently demonstrated that the transcription factor nuclear factor-erythroid 2, which is critical for erythroid maturation and globin gene expression, plays an important role in the pathophysiology of myeloproliferative neoplasms. Myeloproliferative neoplasm patients display elevated levels of nuclear factor-erythroid 2 and transgenic mice overexpressing the transcription factor develop myeloproliferative ... More
Sirtuin-1 protects hair follicle stem cells from TNFa-mediated inflammatory stress via activating the MAPK-ERK-Mfn2 pathway.
Authors:Liu J, Xu Y, Wu Q, Ding Q, Fan W
Journal:Life Sci
PubMed ID:30292830
Stem cell transplantation is a promising tool to treat burn injuries. However, the inflammatory microenvironment in damaged skin limits the efficiency of stem cell-based therapy via poorly understood mechanisms. The aim of our study is to explore the contribution and mechanism of Sirtuin-1 (Sirt1) in TNFa-mediated inflammatory stress in hair ... More
Tanshinone IIA reduces SW837 colorectal cancer cell viability via the promotion of mitochondrial fission by activating JNK-Mff signaling pathways.
Authors:Jieensinue S, Zhu H, Li G, Dong K, Liang M, Li Y
Journal:BMC Cell Biol
PubMed ID:30253740
Mitochondrial homeostasis has been increasingly viewed as a potential target of cancer therapy, and mitochondrial fission is a novel regulator of mitochondrial function and apoptosis. The aim of our study was to determine the detailed role of mitochondrial fission in SW837 colorectal cancer cell viability, mobility and proliferation. In addition, ... More
Artesunate-induced mitophagy alters cellular redox status.
Authors:Zhang J, Sun X, Wang L, Wong YK, Lee YM, Zhou C, Wu G, Zhao T, Yang L, Lu L, Zhong J, Huang D, Wang J
Journal:Redox Biol
PubMed ID:30196190
Artesunate (ART) is a prominent anti-malarial with significant anti-cancer properties. Our previous studies showed that ART enhances lysosomal function and ferritin degradation, which was necessary for its anti-cancer properties. ART targeting to mitochondria also significantly improved its efficacy, but the effect of ART on mitophagy, an important cellular pathway that ... More
Ziram, a dithiocarbamate fungicide, exhibits pseudo-cytoprotective actions against oxidative stress in rat thymocytes: Possible environmental risks.
Authors:Kanemoto-Kataoka Y, Oyama K, Oyama TM, Ishibashi H, Oyama Y
Journal:Environ Res
PubMed ID:29028487
Ziram, a dithiocarbamate fungicide, protects various vegetables and fruits against infections by fungus. Recently, there have been increasing anxieties about the risks in the use of dithiocarbamate fungicides. Our previous studies showed that Zn ... More