CaptureSelect™ FcXP GoPure™ Column

The pre-packed column contains CaptureSelect FcXP Affinity Matrix, which recognizes the CH3 domain of human IgG antibodies, enabling purification of all subclasses (1, 2, 3, and 4) of human IgG and Fc fusion proteins directly from complex source materials

Features of this affinity matrix include:

• High recovery and purity of the target protein directly in a single step
• Mild elution, to retain biological activity and prevent aggregation of Fc fusion proteins and IgG
• Specific for human antibodies; works in the presence of serum (bovine/horse)
• Non-animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

Matrix: agarose-based, epoxide-activated

Average particle size: 65 ± 10 μm

Ligand: CaptureSelect FcXP affinity ligand

Binding capacity: 40–50 mg IgG/mL, depending on residence time

Elution conditions: 20 mM citric acid or acetic acid, pH 3–4,

or 100 mM Tris, 2.0 M MgCl₂, 40% (v/v) propylene glycol, pH 7

Column dimensions: 7 x 25 mm (1 mL column); 16 x 25 mm (5 mL column)

Max. recommended flow: 5 mL/min. (1 mL column); 10 mL/min. (5 mL column)

Max. operation pressure: 2 bar

Formulation buffer: 20% (v/v) ethanol